RNA interference
The RNA interference revolution
... double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell ...of RNA ...
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Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
... The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp (CCA) is used by both nuclear and ...
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Strand Analysis, a free online program for the computational identification of the best RNA interference (RNAi) targets based on Gibbs free energy
... The RNA interference (RNAi) technique is a recent technology that uses double-stranded RNA molecules to promote potent and specific gene ...on RNA-induced silencing complex (RISC) activity and ...
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Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference.
... Mitosis is the evolutionarily conserved process that enables a dividing cell to equally partition its genetic material between the two daughter cells. The fidelity of mitotic division is crucial for normal development of ...
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Knockdown of E2f1 by RNA interference impairs proliferation of rat cells in vitro
... Based on these results, two clones, C6shE2f1-B7 and -B11, were chosen to test E2f1 expression by real time PCR. A comparative analysis of E2f1 expression between two C6shE2f1-B clones (B7 and B11) and control clone ...
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Inhibitors of MyD88-dependent proinflammatory cytokine production identified utilizing a novel RNA interference screening approach.
... One of the hurdles in large-scale gene knockdown experiments is the development of a robust screening system to identify shRNAs with the intended effect. The need for a robust screening system becomes increasingly ...
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Reversible suppression of cyclooxygenase 2 (COX-2) expression in vivo by inducible RNA interference.
... developed RNA interference system in ...inducible RNA interference to target COX-2 expression, we demonstrate potent, reversible Cox2 gene silencing in ...
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Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
... RNA interference (RNAi) is an endogenous protein suppression mechanism by which short double-stranded RNA (dsRNA) mediates sequence-specific degradation of mRNA, thereby preventing translation of the ...
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Modulating drug resistance by targeting BCRP/ABCG2 using retrovirus-mediated RNA interference.
... Some researchers have applied the in vitro chemical synthesis siRNA method to explore the reversal of P-gp-mediated multidrug resistance, which has provided a basis for the operability of this technique [20]. The ...
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Bivalent RNA Interference to Increase Isoflavone Biosynthesis in Soybean (Glycine max)
... bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine ...
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Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma
... activates transcription of the target genes of proliferation, anti-apoptosis, or cell cycle progression by binding directly to high-affinity DNA binding sites or by associating with other transcription factors. In ...
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RNA interference of gonadotropin-inhibitory hormone gene induces arousal in songbirds.
... of RNA interference (RNAi) of the GnIH gene on the behavior of white-crowned sparrows, a highly social songbird ...interfering RNA against GnIH precursor mRNA into the third ventricle of male and ...
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HIV-1 RNAs are Not Part of the Argonaute 2 Associated RNA Interference Pathway in Macrophages.
... of RNA degradation generate 3’ends which are not suitable for our C-tailing procedures applied for the quantification of the HIV-1 sncRNAs ...cellular RNA degradation products in our sequence reads does ...
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RNA interference of myocyte enhancer factor 2A accelerates atherosclerosis in apolipoprotein E-deficient mice.
... Eighty apolipoprotein E-deficient mice (APOE KO) were randomly allocated to control, scramble and MEF 2A RNA interference (RNAi) groups, and constrictive collars were used to induce plaque formation. Six ...
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Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway.
... response, RNA interference (RNAi), is an important modulator of mosquito ...double-stranded RNA (dsRNA), which occurs in the cytoplasm as a result of positive-sense RNA virus infection, ...
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RNA interference and Register Machines (extended abstract)
... RNA interference (RNAi) is a mechanism whereby small RNAs (siRNAs) directly control gene ex- pression without assistance from ...stranded RNA (dsRNA) to be cleaved into ...
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Comparison of dengue virus type 2-specific small RNAs from RNA interference-competent and -incompetent mosquito cells.
... exogenous RNA interference (RNAi) pathway is an important antiviral defense against arboviruses in mosquitoes, and virus-specific small interfering (si)RNAs are key components of this ...
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Functional characterization of a juvenile hormone esterase related gene in the moth Sesamia nonagrioides through RNA interference.
... Juvenile hormone esterase (JHE) is a carboxylesterase that has attracted great interest because of its critical role in regulating larval to adult transition in insects and other arthropods. Previously, we characterized ...
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Cooler temperatures destabilize RNA interference and increase susceptibility of disease vector mosquitoes to viral infection.
... in RNA silencing at this time would be expected to reduce the ability of initially infected cells to mount a response early in the infectious process; the end result being an increase in the number of productive ...
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α-Fetoprotein promoter-driven Cre/LoxP-switched RNA interference for hepatocellular carcinoma tissue-specific target therapy.
... interfering RNA (siRNA) and vector-based short hairpin RNA (shRNA) driven by Pol III promoters (U6 or ...interfering RNA can be delivered through lentiviral vector in a form of shRNA driven by a Pol ...
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