Stable expression
Stable expression of Mycobacterium bovis antigen 85B in auxotrophic
... protein expression was in- duced with 1 mM isopropyl β-D-1-thiogalactopyranoside ...and expression in the cell fractions was analysed by 15% sodium dode- cyl sulphate-polyacrylamide gel electrophoresis ...
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pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.
... We have constructed a small and highly efficient binary Ti vector pSiM24 for plant transformation with maximum efficacy. In the pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank ...
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STABLE EXPRESSION AND POTENTIAL USE OF WEST NILE VIRUS ENVELOPE GLYCOPROTEINS preME AS ANTIGEN IN DIAGNOSTIC TESTS Juliana Felipetto Cargnelutti
... cytomegalovirus (CMV) promoter (9). In addition to the expression cassette, the plasmid contains a selection marker for amplification in E.coli (ampicillin resistance) and a resistance marker for neomycin ...
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Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).
... the expression pattern of functional genes in ...transcript expression levels of target genes, but optimal internal standards are key factors for its accuracy and ...most stable expression ...
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Targeting MAGE-C1/CT7 Expression Increases Cell Sensitivity to the Proteasome Inhibitor Bortezomib in Multiple Myeloma Cell Lines
... MAGEC1/CT7 expression is restricted to malignant plasma cells and it has been suggested that MAGE-C1/CT7 might play a pathogenic role in MM; however, the exact function this protein in the pathophysiology of MM is ...
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Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).
... for stable expression across six organ types at up to three stages of maturity in vernalised ...for stable gene expression with a greater number of factors, including: firstly, testing for ...
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Investigating Immunohistochemical Expression of cyclooxygenase-2 in oral lichen planus
... the expression of cox-2 in lichen planus and control group was not significant ...increased expression cox2 significantly in normal epithelium lichen planus compared with oral mucosa ...increased ...
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Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... the expression values of the set of control genes ...average expression stability (M) and pairwise variation ...raw expression Ct values, generated with the miner algorithm, for each tested gene, in ...
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Reference gene selection for gene expression analysis of oocytes collected from dairy cattle and buffaloes during winter and summer.
... gene expression is a powerful technique for understanding the factors affecting developmental hindrance in oocytes, analysis by real-time reverse transcription PCR (RT-PCR) relies on the correct normalization by ...
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Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.
... gene expression levels, it is essential to normalize real-time quantitative PCR (RT-qPCR) data with suitable internal reference ...transcript expression levels of the target genes have not been previously ...
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The regulation of miRNA-211 expression and its role in melanoma cell invasiveness.
... for stable expression of miR-211 and confirmation of expression by qRT-PCR analysis (see ...artificial expression of miR-211 in both melanoma cell lines (Figure ...the expression of ...
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Promoting stable post-conflict education
... When the NGO won a $4.5m USAID grant in 1994 the pre-school design became more elaborate. Emphasis on providing quality education in stable, safe spaces increased and training continued for some pre-school teach- ...
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Heterokaryon-based reprogramming of human B lymphocytes for pluripotency requires Oct4 but not Sox2.
... Gene expression in reprogrammed lymphocytes resembles human rather than mouse ES cell ...gene expression in several human (NCL1, H1, H7 and H9), mouse (OS25, CCE, E14 and ZHBTc4) ES cell lines and in ...
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Clonagem e expressão do fator 1 humano induzível por hipóxia (HIF-1) na levedura Saccharomyces cerevisiae
... levels, stable HIF-1 α and ARNT translocate into the nucleus, form the heterodimer and bind to the DNA recognition site leading to the expression of several target genes, of which over one hundred have been ...
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Distant stable direct orbits around the Moon
... Due to the perturbation of the third body (Earth), the two- body energy (Moon-particle) is not constant. By monitoring E, it is possible to have a clue of which primary has the dom- inant gravitational influence on the ...
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Proteomic screening of human targets of viral microRNAs reveals functions associated with immune evasion and angiogenesis.
... NanoRPLC–MS/MS analysis was performed using an Agilent 1100 nanoflow LC system coupled with hybrid linear ion trap- fourier transform ion cyclotron resonance (LIT-FTICR) mass spectrometer (LTQ FT Ultra) (ThermoElectron, ...
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Alterations in the expression of the NF-κB family member RelB as a novel marker of cardiovascular outcomes during acute exacerbations of chronic obstructive pulmonary disease.
... IL-8 expression via the NF-kB pathway by suppressing gene transcription ...relative expression levels we report may be an ...protein expression remains to be determined. However, RelB protein ...
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HOXA5 inhibits metastasis via regulating cytoskeletal remodelling and associates with prolonged survival in non-small-cell lung carcinoma.
... The expression of the Arp2/3 complex has been found to be associated with malignant phenotypes in certain types of cancer cells ...Arp2/3 expression might contribute to migration and invasion in malignant ...
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Meta-stable Solidification Created by the Detonation Method of Coating Formation
... A hypothesis dealing with the inter-metallic phases formation in such a single particle of the nominal composition N 0 0.63 is presented. At first, the solid / liquid system is treated as an interconnection: substrate ...
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The supervision of the credit institutions in the context of Basel III Implementation
... the stable net resources is that indicator that additionally introduces a liquidity control on medium and long ...the stable liquidity appeared as a result of the manifestation of the financial crisis, and ...
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