[PDF] Top 20 Amplification, cloning and DNA sequencing of the
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Amplification, cloning and DNA sequencing of the
... is the causative agent of East Coast Fever (ECF), a tick borne disease, which results in major economic losses in ...are the high cost of drugs, development of resistance, and ... See full document
6
DNA extraction and amplification conditions
... The development of new grapevine cultivars adapted to Brazilian tropical and subtropical conditions has been one of the main objectives of genetic breeding programs in ... See full document
10
DNA extraction, PCR, and DNA sequencing
... Moreover, the separation of the ...members of this complex, indicates that the collection sites in northeast and southeast Brazil exhibit individuals of a single ...using ... See full document
9
OPTIMIZATION OF RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUES FOR USE IN GENETIC STUDIES OF CUBAN TRIATOMINAE
... concentrations of MgCl 2 (1.5 to 6.0 mM) in the PCR mixture resulted in both quantitative differences in RAPD derived DNA and qualitative changes in the DNA ...patterns. ... See full document
6
Hb Köln [a2b298(FG5) val-met] identified by DNA analysis in a Brazilian family
... by DNA analysis in a Brazilian ...2 and S, in hemoglobin electrophoresis on a cellulose acetate membrane, and a band with electrophoretic migration similar to Hb C on agar ...instability and ... See full document
5
Digital Droplet Multiple Displacement Amplification (ddMDA) for Whole Genome Sequencing of Limited DNA Samples.
... improved the quality of whole genome amplification compared to a conventional MDA reaction, which was attributed to discretization of template DNA by parti- tioning into numerous small ... See full document
12
Análise microbiológica de infecções endodônticas utilizando a associação das técnicas do Multiple Displacement Amplification (MDA) e da Hibridização DNA-DNA (Checkerboard)
... vacuum and fixed onto the membrane by crosslinking using ultraviolet light (Stratalinker 1800, La Jolla, CA) followed by baking at 120˚C for 20 ...min. The Minislot device permitted the ... See full document
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Comparison of protocols for the extraction of genomic DNA from Sharpshooters (Hemiptera: Cicadellidae) for the detection of Xylella fastidiosa
... on the use of primers that allow the detection of as few as 10 to 100 bac‑ teria per amplification reaction (POOLER; HARTUNG, ...1995). The use of this technique to detect ... See full document
5
Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep
... digestion of the purified plasmids was performed using BamHI (10 U/µL, Promega, Madison, WI, USA) and HindIII (10 U/µL, MBI Enzymatic Fermenta, Hanover, MD, USA) at 37 °C for 1 ...determine the ... See full document
5
cDNA cloning and 1.75 Å crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds
... sequences of plant chitinases YWGQNGG and WVQFY NNP (sense primer 5¢-TAY TGG GAR AAY GGN GG-3¢, and antisense primer 5¢-GG RTT RTT RTA RAA YTG NAC CCA-3¢; the nomenclature follows the ... See full document
13
Variability in Ribosomal DNA Genic and Spacer Regions in Verticillium dahliae Isolates from Different Hosts
... with the primer pair ITS1/ITS4 as described by White et ...screening of candidate restriction enzymes, digestion of amplification products was performed using the restriction enzymes ... See full document
6
Improved resolution of reef-coral endosymbiont (Symbiodinium) species diversity, ecology, and evolution through psbA non-coding region genotyping.
... amplifying and directly sequencing the non-coding region of the plastid psbA minicircle using the primers ...alternative, the universal primers psbAFor_1 (5 9- GCA GCT CAT ... See full document
11
PCR AMPLIFICATION AND SEQUENCING
... Within the entomofauna that participates in the processes of cadaverous putrefaction, the family Calli- phoridae is of particular interest because their carrion habits makes it one ... See full document
7
An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates
... using the multilocus se- quence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), ... See full document
9
An empirical approach for quantifying loop-mediated isothermal amplification (LAMP) using Escherichia coli as a model system.
... as the maximum rate of amplification of the ...as the change in concentration over unit time, which graphically is the slope of the Richard’s curve at its ... See full document
10
DNA extraction, amplification and sequencing
... from the type locality in Guyana. While the Brazilian specimen agrees well both macro- and micromorphologically with the Guyana type specimen, minor differences include the smaller ... See full document
10
Specific RNA Interference in Caenorhabditis elegans by Ingested dsRNA Expressed in Bacillus subtilis.
... variety of different microbes, such as Bacilli (B. subtilis and related bacteria), Staph- ylococci, Streptomyces, Micrococcus luteus, Comamonas and many Pseudomonas species [11– ...Ausubel and ... See full document
15
An alternative method for Staphylococcus aureus DNA isolation
... by the production of a wide variety of enterotoxins ...in the production of skin lesions in neonates (Jaffe et ...52% of the strains isolated from bovine mastitis produced ... See full document
8
Arq. NeuroPsiquiatr. vol.52 número3
... DNA amplification by the polymerase chain reaction for the rapid diagnosis of tuberculous meningitis: comparison of protocols involving three mycobacterial DNA sequences, IS6110, 65 KD[r] ... See full document
2
Diversity of Phytophthora species in natural ecosystems of Taiwan and association with disease symptoms
... parvispora and P. tropicalis, and 14 previously unknown Phytophthora taxa in Clades 2, 6, 7 and 9 recovered from 19 of the 25 rivers and streams tested riparian Phytophthora ... See full document
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