[PDF] Top 20 Cloning, Expression, Purification and Assay of Sorbitol dehydrogenase from “Feicheng” Peach Fruit (Prunus persica)
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Cloning, Expression, Purification and Assay of Sorbitol dehydrogenase from “Feicheng” Peach Fruit (Prunus persica)
... cv. Feicheng) were obtained from Feicheng, Shandong province in China at a pre-climacteric but physiological mature stage, with a mean firmness of 85 N cm −2 ...uniformity of size ... See full document
10
Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase
... plasmids, and culture media A novel strain of ...lab from poul- try slaughterhouse effluent, possessing well-established lipase activity, and identified by 16S rRNA gene sequenc- ing (GenBank ... See full document
9
Cloning and expression of NS3 helicase fragment of hepatitis C virus and the study of its immunoreactivity in HCV infected patients
... cause of liver failure ...effective and produce side effects in most ...(NS3) of HCV is one of the key enzymes in viral replication and ...drugs and therapies against HCV ... See full document
5
Cloning and Expression of Helicobacter pylori HpaA Gene
... prokaryotic expression systems has been construct- ed in some previous ...methods and vectors for cloning and expression of this ...Xu and coworkers constructed a live ... See full document
4
Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose
... samples from 6-month-old saplings and from 3- and 22-year-old plants were used for total RNA isolation, as described by Salzman et ...purified from 75 mg of total RNA, using the ... See full document
13
Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
... genes of Danio rerio and Ciona intestinalis in which no duplicated regions have been ...function of this region can be realized by the spatial interaction of differ- ent conserved motifs (C ... See full document
7
Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China
... – Expression and purification of the recombinant protein Der f ...Confirmation of pET28a (+)-Der f 2 plasmid by restriction enzyme ...subcloning and blue/white screening, the ... See full document
11
Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp ( Penaeus monodon)
... study of molecular regulatory mechanisms re- lated to promotion of reproductive development and matu- ration have begun to receive more attention,, especially in shrimp ...series of complex ... See full document
11
Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation
... Isolation and identification of the MLC cDNA clones MLC cDNAs were isolated after screening a cDNA library prepared from sea bream larvae 1–10 days after ...frame of 513 nucleotides, starting ... See full document
10
Cloning, expression and purification of a sea urchin adhesive protein towards the development of a new biomedical adhesive
... Nectin and Toposome that had never been reported in the adult adhesive ...proteome and the phospho- and ...cluster of seven spots in the central upper part of the gels, which is in ... See full document
52
KEY WORDS: Spider, Lasiodora sp, cloning, purification, expression, antimicrobial,
... Pain and itch sensations are induced by depolarization of C-fibre nerves and possibly other types of ...evidence from several species, including mice, that skin plasma extravasation ... See full document
50
Clonagem e expressão heteróloga do gene de uma xilanase de Thermoascus aurantiacus em Pichia pastoris
... Cloning, expression, characterization, and high cell-density production of recombinant endo-1,4- β -xylanase from Aspergillus niger in Pichia pastoris. Biotechnology o[r] ... See full document
75
Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli
... sequenced and transferred to the bacterial expression vector pET21a (data not ...kinetics of urate oxidase expression, a colony of ...mid-log and induced with IPTG for 0, 15, 30, ... See full document
6
Wei Lei, Shao-Hu Tang, Ke-Ming Luo and Min Sun
... cloned and characterized an svchs gene from the deciduous medicinal herb ...proteins from other plants, especially ...malonyl-CoA and an N-myristoylation ...level, and this could ... See full document
8
Clonagem, superexpressão, purificação e caracterização da proteína recombinante humana fator estimulador de colônias de granulócitos
... Synthesis of G/CSF coding DNA sequence was carried out by a method developed in our laboratory, to which a patent has been filed ...sequence of hG/CSF, without making any modification such as replacement ... See full document
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Innate Host Habitat Preference in the Parasitoid Diachasmimorpha longicaudata: Functional Significance and Modifications through Learning.
... endoparasitoid of Tephritidae lar- vae. In its habitat of origin, ...larvae of several species of the genus Bac- trocera [50,51], while they are feeding inside the ...Most of its hosts ... See full document
18
Increased migration of monocytes in essential hypertension is associated with increased transient receptor potential channel canonical type 3 channels.
... immunoblotting of TRPC3 in monocytes lysates to test the specificity of the antibody for TRPC3 by antigen competition experiments is shown in Figure ...protein expression in monocytes from ... See full document
13
Overexpressing 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) in the lactococcal mevalonate pathway for heterologous plant sesquiterpene production.
... Production of Recombinant PMSTS and HMGR Enzymes The recombinant PMSTS from the pNZ:PMSTS plasmid and PMSTS together with HMGR from the pNZ:PMSTS:mvaA plasmid were analysed by SDS-PAGE ... See full document
10
Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli
... tubes and IPTG was added to each subculture with different final concentrations (0, ...optimization of IPTG concentration. For the optimization of expression temperature, the tubes were ... See full document
6
Relationship between fruit traits and contents of ascorbic acid and carotenoids in peach
... ED and PD (above ...between fruit mass, equatorial diameter and polar diameter was strong and positive (above ...between peach traits, and found strong positive correlations ... See full document
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