[PDF] Top 20 A conglutination test for rapid detection of antibodies against Babesia bigemina

... spite of these improvements, the sensitivity obtained was not superior to that of the indirect fluorescent antibody technique ...desirable test characteristics besides specificity and sensitivity ... See full document

... number of copies of the gene cytochrome b (mt-cytB) of both babesias and Anaplasma msp 1b gene in all ...Merozoites of B. bigemina were seen in blood smears of three calves from ... See full document

... preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite ...dilutions of the ... See full document

... preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM ...dilutions of the antigen, using positive and negative reference sera, were determined by ... See full document

... with Babesia bigemina ...results of both tests (P= ...region of the State of Mato Grosso do Sul with 1,365 cattle sera, ...for antibodies against ... See full document

... relatively rapid procedure (Diaz & Varela- Diaz ...rescent-antibody test and the RFFIT (Diaz & Myers 1980, 1981, Diaz ...lots of suckling mouse ra- bies and tissue culture rabies vaccines ... See full document

... excess of antigen was removed by three washes with phosphate bufer solution (PBS) plus ...L of PBST 20 containing 5% skimmed milk was added for 90 minutes at 37 °C and washed of three times with PBST ... See full document

... sensitivity of the probe pairs, we isolated total RNA directly from ...limit of detection (LOD) of the biosensor assay for ...dilutions of the purified RNA were prepared and ... See full document

... aim of this study was to detect antibodies against Paracoccidioides brasiliensis in dogs seropositive and seronegative for ...immunodiffusion test using gp43 and exoantigen, respectively. The ... See full document

... chlamydial antibodies in sheep flocks in Costa ...Levels of seroprevalence determined in the different regions ...levels of seroprevalence (Jiménez- Estrada et ...mobilization of animals from ... See full document

... production of animal food for human consumption in most parts of the ...development of viable animal industries wherever they ...disease of sheep and goats is widespread in the inter-tropical ... See full document

... sample of milk, a loopful of MAP positive control was inoculated in 50 mL of milk powder Molico® (Nestlé, São Paulo, Brazil) reconstituted with distilled water and autoclaved, according to the ... See full document

... dos. Foram avaliadas as alterações clínicas e hematológicas por meio da determinação da parasitemia, do hemograma, do fibrinogênio plasmático, da contagem de reticulócitos, da análise descritiva da medula óssea e da ... See full document

... fabrication of GCE modified with AuNPs was adapted from previously published reports [31] with some ...surface of electrode was polished with 8 nm aluminum oxide powder on 1,000 mesh ...Modification ... See full document

... B. bigemina não diferiram e que ambas eram também semelhantes em bezerros e ...da Babesia bigemina, provocando a ocorrência de instabilidade enzoótica em um sistema estável e com isso aumentar o ... See full document

... infectividade da amostra de A. centrale foi comprovada pela detecção de parasitemia patente e presença de anticorpos específicos em 100% dos animais. O período pré-patente médio de 35,3 dias é comparável ao observado por ... See full document

... signs of TRT but titre of antibodies against TRT/SHS according to ELISA results were clearly positive in ...ELISA test to examine 16 flocks in which 14 had birds with respiratory signs ... See full document

... use of chicken IBV-specific g-globu- lin as capture antibody has several advan- tages over the use of rabbit polyclonal anti- ...anti-IBV antibodies in broiler chickens as frequently as the ... See full document

... role of Helicobacter pylori in chronic rhinosinusitis: A preliminary ...H. Detection of Helicobacter pylori in nasal and maxillary sinus specimens from patients with chronic ... See full document

... panel of 13 MAbs. For the indirect ELISA, 0.5 mL of each selective enrichment broth used in the conventional detection methodology were inoculated in a tube containing 10 mL of BHI broth and a ... See full document