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[PDF] Top 20 DNA qualification workflow for next generation sequencing of histopathological samples.

Has 10000 "DNA qualification workflow for next generation sequencing of histopathological samples." found on our website. Below are the top 20 most common "DNA qualification workflow for next generation sequencing of histopathological samples.".

DNA qualification workflow for next generation sequencing of histopathological samples.

DNA qualification workflow for next generation sequencing of histopathological samples.

... Histopathological samples are a treasure-trove of DNA for clinical ...quality of DNA can vary depending on the source or extraction method ...cost-effective workflow for ... See full document

8

Unveiling distribution patterns of freshwater phytoplankton by a next generation sequencing based approach.

Unveiling distribution patterns of freshwater phytoplankton by a next generation sequencing based approach.

... correspondence of NGS data to microscopic biovolume estimates compared to abundances (Table 3) is likely explained by variations in the number of chloroplasts per cell (and corresponding number of ... See full document

10

Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

... Taq DNA polymerase ...cycles of 94uC for 40 s, 56 uC for 1 min, and 72uC for 1 min with a final extension at 72 uC for 10 ...environmental samples (1, surface soil-1; 2, surface soil-2; 3, Drinking ... See full document

6

Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis.

Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis.

... complicates DNA extraction procedures, often leading to contamination of MTB lysates with residual mycolic acid ...all of our genomic DNA samples showed evidence of some form ... See full document

14

Next-generation phylogeography: a targeted approach for multilocus sequencing of non-model organisms.

Next-generation phylogeography: a targeted approach for multilocus sequencing of non-model organisms.

... heterozygous samples, they were on average a low percentage of reads ...because of heterozygous chimeric sequences ...454 sequencing, and would only effect heterozygous ...percentage of ... See full document

10

Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F.

Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F.

... patients of both sexes and five non-penetrant carriers (93% ...exome sequencing analysis, we selected four affected family members (VII-5, VI-53, V-28, and VI-36) with a manifest LGMD phenotype separated by ... See full document

7

The accuracy, feasibility and challenges of sequencing short tandem repeats using next-generation sequencing platforms.

The accuracy, feasibility and challenges of sequencing short tandem repeats using next-generation sequencing platforms.

... populations of Saccharomyces cerevisiae derived from the haploid DH89 a, ho strain, which in turn derives from the laboratory Y55 wildtype strain ...genomic DNA was extracted from yeast culture regrown in a ... See full document

14

Quantifying Next Generation Sequencing Sample Pre-Processing Bias in HIV-1 Complete Genome Sequencing

Quantifying Next Generation Sequencing Sample Pre-Processing Bias in HIV-1 Complete Genome Sequencing

... and sequencing technologies were quickly adopted by HIV researchers in order to broaden the understanding of the clinical importance of minor drug-resistant ...genome sequencing an important ... See full document

14

Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

... application of NGS to FFPE material. One of the first came from Schweiger et ...analysis of DNA (DNA-Seq) isolated from FFPE samples ...cancer samples from a single ... See full document

16

Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes.

Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes.

... number of whole genome shotgun sequencing libraries processed at different sequencing centers by a standardized protocol and not expected to contain etiologic microbes or be complicated by issues ... See full document

8

DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.

DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.

... quantity of the limited tissue samples ( ,1,5 g; Table 1) was homogenized in about 1500 m L PBS per g of tissue using gentle homogenization in a TissueLyser ...enriched samples were subjected ... See full document

6

Development of a low bias method for characterizing viral populations using next generation sequencing technology.

Development of a low bias method for characterizing viral populations using next generation sequencing technology.

... with DNA barcoding to identify and quantify the frequency of rare HIV drug resistance mutations in patient plasma samples ...utility of 454 deep sequencing technology to identify minor ... See full document

9

An alternative storage method for characterization of the intestinal microbiota through next generation sequencing

An alternative storage method for characterization of the intestinal microbiota through next generation sequencing

... subject of various molecular studies mainly due to its importance and wide-ranging relationships with human ...storage of fecal samples prior to DNA extraction is critical when characterizing ... See full document

11

Systematic comparison of three methods for fragmentation of long-range PCR products for next generation sequencing.

Systematic comparison of three methods for fragmentation of long-range PCR products for next generation sequencing.

... Genomic DNA was isolated from peripheral human blood cells by salt ...amplification of extra-long templates of up to 20 ...test samples, three for each fragmentation method. Each of the ... See full document

6

Molecular diagnosis of Usher syndrome: application of two different next generation sequencing-based procedures.

Molecular diagnosis of Usher syndrome: application of two different next generation sequencing-based procedures.

... patient samples, which previously turned out to be negative for known mutations in Usher genes as assessed by APEX microarray screening ...sites of known Usher ...specific workflow as for the ... See full document

10

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples

... - DNA from BrC, BrC tumour-ad- jacent controls, GC, GC tumour-adjacent controls, and NAG was ...Pools of five patients formed every group of study for a total of five ...μg of ... See full document

9

Development of a new pharmacogenomics system for lung cancer based in next generation sequencing

Development of a new pharmacogenomics system for lung cancer based in next generation sequencing

... role of molecular aberrations as the cause for the occurrence of ...application of precision medicine may allow the prevention of many diseases while also reducing the costs and toxicity ... See full document

14

Authentication of Herbal Supplements Using Next-Generation Sequencing.

Authentication of Herbal Supplements Using Next-Generation Sequencing.

... use of ITS2 as a reference marker for two of the species studied was hampered by two factors: primer specificity (Ginkgo biloba) and intraspecific variability (Valeriana ...design of a new ... See full document

24

Molecular typing of lung adenocarcinoma on cytological samples using a multigene next generation sequencing panel.

Molecular typing of lung adenocarcinoma on cytological samples using a multigene next generation sequencing panel.

... Identification of driver mutations in lung adenocarcinoma has led to development of targeted agents that are already approved for clinical use or are in clinical ...number of biomarkers that will be ... See full document

6

Leukemia gene atlas--a public platform for integrative exploration of genome-wide molecular data.

Leukemia gene atlas--a public platform for integrative exploration of genome-wide molecular data.

... wealth of high-throughput data has been published. But further utilization of these data by researchers is severely hampered by the lack of accessible integrative tools for viewing and ...analysis ... See full document

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