[PDF] Top 20 Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
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Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
... osa and Andana were used. Colletotrichum acutatum, a major strawberry pathogen was used for natural infection and controlled ...culture and growth conditions, C. acutatum experimental ... See full document
18
Identification of reference genes for relative quantification of circulating microRNAs in bovine serum.
... microRNAs in body fluids have been implicated as promising biomarkers for physiopathology ...levels of circulating microRNAs are estimated by reverse transcription quantitative real-time polymerase chain ... See full document
12
Identification of valid reference genes for the normalization of RT-qPCR expression studies in human breast cancer cell lines treated with and without transient transfection.
... measuring transcript abundance due to its sensitivity, reproducibility, simplicity and high-throughput ...is normalization of gene expression using an endogenous reference ...Ideal ... See full document
15
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... higher in resistant than in susceptible strain had been previously found by Lou et al [21] using Western blotting and Vanadate-sensitive ATPase ...the reference genes a-Tubulin, TBP ... See full document
9
Selection and Validation of Reference Genes for Accurate RT-qPCR Data Normalization in Coffea spp. under a Climate Changes Context of Interacting Elevated [CO2 ] and Temperature
... specificity of the primer pairs used in this study, melting curve analysis was performed after 40 cycles of ...specificity of the amplicon. No signal was detected in the negative ... See full document
11
Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... used in this study, one might wonder which one could lead to the most reliable ...ranking of reference genes [44,47]. This package uses the matrix of rank-ordered genes as an ... See full document
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Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without Kranz anatomy
... networks and identify stress-responded genes, high throughput gene expression analysis should be considered (Delporte et ...one of the most widely used technologies to validate small sets of ... See full document
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... The validation of reference genes has been carried out in several cucurbitaceous crops, including melons, cucumbers, and zucchi- ...nis. In cucumbers, EF1a, Fbox, CAC ... See full document
11
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.
... rapid and accurate quantification of gene expression ...application of RT-qPCR requires accurate normalization though the use of reference ...single reference gene is ... See full document
14
Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp.
... GAPDH and 18S rRNA, the expression levels of EF-1a and b-actin were found to be more stable with lower variation in threshold cycles (Ct) in this ...for normalization than ... See full document
10
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... several reference gene(s) acting as normalization factor(s). In order to facilitate gene expression studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome ... See full document
10
Validation of commonly used reference genes for sleep-related gene expression studies
... (PSD96) and animals subjected to PSD96 and then a sleep recovery period of 24 hours ...(SR24). In the second experiment, a group of animals subjected to total sleep deprivation for 6 ... See full document
8
RFECS: a random-forest based algorithm for enhancer identification from chromatin state.
... accuracy of the enhancer prediction by RFECS, we applied this algorithm to chromatin profiles of 24 marks obtained in H1 and ...the validation rate as the percentage of predicted ... See full document
14
Identification of Suitable Reference Genes for Gene Expression Studies of Shoulder Instability
... combination of reference genes, we also quantified the mRNA expression of a target gene, COL1A1 using the candidate reference genes for ...chain of human procollagen type ... See full document
9
ISSN 0100-879X BIOMEDICAL SCIENCES AND CLINICAL INVESTIGATION
... involvement of conserved molecular components and the innate immunity response in stomatal defense against invading bacteria suggest that this form of defense may be widespread ... See full document
8
Eucalyptus ESTs involved in mechanisms against plant pathogens and environmental stresses
... Proteins of the PR-10 family seem to be ubiquitous in plants, occurring in several plant ...apple, and celery share sequence homologies with PR-10 family and are also included in ... See full document
9
Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
... changes in gene expression induced by abiotic stress ...membrane and hybridized with a detectable complementary probe ...consuming and labor intensive. And although the sensitivity threshold ... See full document
10
Distinct roles of jasmonates and aldehydes in plant-defense responses.
... levels of these metabolites remained unchanged as compared to that of the wild ...lesions in these two independent Figure 6. Attraction of parasitoid wasp, Aphidius colemani, to the in ... See full document
10
Novel galanin receptors in teleost fish: identification, expression and regulation by sex steroids
... Transcript levels of the four GALR genes in immature male sea bass brain and testis in response to.. 19.[r] ... See full document
28
Criterion for selection the optimal physical and chemical properties of cobalt aluminate powder used in investment casting process
... quality of ceramic moulds and in consequence the quality of the castings depends on the thickness of the ceramic layer applied on the wax ...pattern. In turn, the thickness ... See full document
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