[PDF] Top 20 MATERIALS AND METHODS Cultivation of the microorganism
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MATERIALS AND METHODS Cultivation of the microorganism
... organic and inorganic nitrogen in the medium were measured during ...under the same conditions, a mean cell nitrogen percentage of ...to the dry biomass was found. The inorganic ... See full document
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MATERIALS AND METHODS Cultivation of the microorganism
... concentrations of substrates and low pH (13). The cells developed under these conditions exhibited an extended “lag” phase, and after adaptation, their growth was relatively fast (about 2 to 3 ... See full document
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MATERIALS AND METHODS Microorganism and growth properties
... (2) and proteases activity was expressed as Anson ...is the amount of enzyme which, under the analytical specified conditions (2% casein as substrate, pH ...hydrolyzed the casein to ... See full document
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MATERIALS AND METHODS Microorganism and culture conditions
... to the genus Bacillus for industrial applications such as ...subtilis and Bacillus licheniformis (22), the latter now being of greater industrial importance ...(7). The properties ... See full document
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MATERIALS AND METHODS Microorganism and culture conditions
... producer of cellulases many of which have been characterized (18). The effect of H + concentration in reaction mixtures on enzymatic activities work in conjunction with other factors within ... See full document
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MATERIALS AND METHODS Microorganism and culture maintenance
... at the 0.05 level. This also corresponded to a 95% of confidence level for test of the hypothesis that the effects (or coefficients) in this situation were equal to ...that the ... See full document
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MATERIALS AND METHODS Microorganism and Culture Conditions
... either the control or other oil- supplemented ...all the oil-grown cultures, which was reflected in their final cell concentrations, GLA production (on volume basis) increased in the culture ... See full document
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MATERIALS AND METHODS Strain Cultivation
... groups of microorganisms, including moulds, bacteria, and ...cellulolytic and xylanolytic enzymes (1, 4). A characteristic feature of the mould strains is their ability to produce ... See full document
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MATERIALS AND METHODS Microorganism and maintenance
... mushrooms of the Pleurotus genus are more delicate and sensitive than the Agaricus genus and they start deteriorating immediately within one day after the ...life of these ... See full document
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MATERIALS AND METHODS Microorganism and culture
... 5.5 and two forms (I e II) were separated. The form I did not interact with the resin, and the fractions with amylolytic activity were pooled and applied to a CM-Celullose ... See full document
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MATERIALS AND METHODS Microorganism and media
... spite of several decades of efforts, the production and productivity of secondary metabolites by cell culture technology is still facing many biological and engineering ...One ... See full document
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MATERIALS AND METHODS Microorganism and culture conditions
... (2). The solvents were added to the dried cells in a sequence starting from chloroform, methanol and distilled water as described by ...mixture of dried cells and solvents was sonicated ... See full document
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MATERIALS AND METHODS Microorganism and enzyme production
... nature and the alkalophilic Bacillus strains are often good sources of alkaline extracellular enzymes (11, ...actinomycetes and bacteria (13, 14). Lipases are of significant importance ... See full document
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MATERIALS AND METHODS Microorganism, culture medium and growth conditions
... at the two highest cell densities, ISM- images exhibited less ...cells and, consequently, to a saturation of the ISM counting rate, which is used to measure cell density (Figure ...brightness ... See full document
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MATERIALS AND METHODS Microorganism maintenance and culture conditions
... . The reaction mixture contained 100 Ql substrate, 800 Ql of buffer 50 mM sodium acetate (pH ...) and 100 L of the culture supernatant (diluted when necessary) for 5 ...min. The ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... NaCl, and 0.02 mM phenyl-methyl-sulfonyl-fluoride (PMSF). The cell suspension was incubated for 30 min in the presence of lysozyme ...pulses of 30 sec each) at 4ºC, and ... See full document
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MATERIALS AND METHODS Microorganism and culture conditions
... inhibit the activity of proteases (e.g., by binding on the active ...However, the protease will recover its full activity when the detergent is diluted in the washing process ... See full document
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The comparison of the structure and microhardness of the tool steel C90 and HS 6-5-2 remelted with the electric arc
... group of materials used for the production, not only tools, but also machine ele- ments, that need to have the increased strength, for example the high-speed steels are used on ... See full document
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MATERIAL AND METHODS Microorganism
... in the breakdown of proteins (25). The microbiology of Brazilian soil, which has special environmental characteristics and is rich in actinomycete populations, has not been very well ... See full document
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MATERIALS AND METHODS Microorganism and growth conditions
... is the petroleum industry, where they have been incorporated into oil formulations and utilized in petroleum production and ...bioremediation. The advantages ascribed to biosurfactants ... See full document
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