[PDF] Top 20 MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells.
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MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells.
... D/D induced stem cell-like cells proliferated slower than control ES and ...these cells failed to differentiate into endoderm with Activin and other growth factors in ... See full document
8
The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
... donor fibroblasts; the selection criteria were again p < ...with fibroblasts (Table ...used mouse neural stem cells (NSCs), which have a high endogenous expression of Sox2, as the ... See full document
8
Expressão de marcadores de células germinativas e de oócitos em fibroblastos bovinos tratados com 5azacitidina e em célulastronco adultas cultivadas in vitro na presença de BMP2, BMP4 ou fluido folicular
... contain cells which can be isolated and propagated, and have the characteristics of oogonial stem ...reintroduction into the ovary, or in reaggregation models, these primitive cells can form ... See full document
210
Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
... stromal cells have been reported [8, 16, 20, ...HSF6 cells differentiated into neural rosette-like cells, which are primitive neuroe- pithelial cells (Fig 1B and 1C, ... See full document
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Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation.
... progenitor cells (NPCs) are ...integrate into the mature neural ...differentiation induced by all-trans retinoic acid (RA) in pluripotent stem cells is accompanied by increased ... See full document
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Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells.
... Human induced pluripotent stem cells (hiPSCs) generated by de-differentiation of adult somatic cells offer potential solutions for the ethical issues surrounding human embryonic ... See full document
10
Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells
... endothelial-like cells at the time heman- gioblasts were ...and mouse embryo begins in the yolk sac, within blood islands that consist of emerging primi- tive erythroblasts surrounded by endothelial ... See full document
14
Comprehensive Identification of Kruppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming.
... in mouse embryonic stem (ES) cells and is induced from somatic cells by the activation of appropriate transcriptional regulatory ...of mouse ES cells as well as in ... See full document
17
Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
... Lineage Cell Depletion Kit (#130-092- 211) ...The cell permeable DNA dye Hoechst 33342 (HO) was included at 10 m g/ mL during the antibody staining to allow the discrimination of nucleated cells (HO ... See full document
11
Mouse-induced pluripotent stem cells differentiate into odontoblast-like cells with induction of altered adhesive and migratory phenotype of integrin.
... iPS cells into odontoblast-like ...ES cells (B), we cultured these cells on various ECM proteins for 14 days, examining cell proliferation at various time points during this ... See full document
14
High glucose-mediated oxidative stress impairs cell migration.
... in cells treated for 3 days with LG or HG medium, both in the presence or absence of N-acetyl-cysteine (NAC, 10 mM, 1 h) [18], an ...antioxidant. Cells in HG medium exhibited a twofold increase in ROS ... See full document
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INDUCED PLEURIPOTENT STEM CELLS: A PIONEER IN THERAPY
... pluripotent stem cells can potentially be used to counter a wide range of diseases, from diabetes to spinal cord injury, to childhood leukemia, to heart ...iPS cells are similar to human ... See full document
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Transplantation and survival of mouse inner ear progenitorstem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results
... receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing ...postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after ... See full document
10
MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor.
... a cell-specific population of epiblast-derived stem cells (EpiSC) which resemble ESc in many characteristics including pluripotency markers and colo- ny morphology ...programmable into iPS ... See full document
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Induction of pluripotent stem cells by reprogramming human ocular fibroblasts under xeno-free conditions
... added into the culture medium for 1 h at ...and cell clones exhibiting fluorescent staining were marked at the bottom of the culture dish using a marker ...live cells in the culture dish/the number ... See full document
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MicroRNA-200a regulates Grb2 and suppresses differentiation of mouse embryonic stem cells into endoderm and mesoderm.
... differentiating embryonic carcinoma cell cultures [43], and Afp expression is dependent on FGFs that are produced by the cardiac mesoderm in embryonic endoderm cells ...specifically ... See full document
12
Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells.
... bright cells were selected based on increased signal intensity above the main ...SSEA1+ cells (Figure ...bright cells relative to cKit mid and cKit dim ...bright cells at day 8 of ... See full document
11
Stem cells – Current concepts and Future applications
... human embryonic stem cells was achieved just over a decade ...using stem cells in a variety of clinical indications but most of these have been Phase I or Phase II ... See full document
2
Prostate cancer cell lines under hypoxia exhibit greater stem-like properties.
... 6 cells) were harvested and suspended in prewarmed RPMI 1640 medium containing 2% fetal bovine serum and 2 mM HEPES ...the cells were washed with ice-cold HBSS with 2% FBS, centrifuged at 4uC, and ... See full document
13
Functional properties of a Brazilian derived mouse embryonic stem cell line
... Pluripotent mouse embryonic stem cells (mESC) are cell lines derived from the inner cell mass of blastocyst-stage early mammalian ...in mouse and human ESC it is ... See full document
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