[PDF] Top 20 Protease Gene Shuffling and Expression in Pichia pastoris
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Protease Gene Shuffling and Expression in Pichia pastoris
... market in the world with an estimated market of about three billion US dollars annually (Leary et ...roles in improving nutrient values, retarding deterioration, preventing undesired interactions, ... See full document
6
Applications of recombinant Pichia pastoris in the healthcare industry
... production and may disagree with the rules imposed by regulatory agencies (Sreekrishna et ...L1 gene and a manner to do this is to use integrative vectors. In addition, the use of an ... See full document
6
Pichia pastoris Like an Expression System
... gelatin in pPIC9 and a multicopy pPIC9K expression vectors of Pichia pastoris was ...gelatin and a ...(Col1a1-1) and a ...medium and the problems of proteolytic ... See full document
6
N-terminal domain of turkey pancreatic lipase is active on long chain triacylglycerols and stabilized by colipase.
... The gene encoding the TPL N-terminal domain (N-TPL), fused with a His6-tag, was cloned and expressed in Pichia pastoris, under the control of the glyceraldehyde-3-phosphate ... See full document
9
Avaliação da atividade antimicrobiana das defensinas recombinantes de ervilha (drr230a) e café (cd1) produzidas em Pichia pastoris
... fungi in agriculture and the frequent incidence of human diseases caused by bacteria which are resistant to antibiotics lead to the search for alternative antimicrobial ...compounds. In this context, ... See full document
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Clonagem e expressão do gene da glicoproteína S1 do Vírus da Bronquite Infecciosa em Pichia pastoris
... variable and responsible for attachment to the receptor in host cellular membrane, induction of neutralizing and haemagglutination-inhibiting antibodies in ...immuno-diagnosis and ... See full document
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Transcriptional profiling of protein expression related genes of Pichia pastoris under simulated microgravity.
... source and ultimately more final cell counts were ...oxidase gene (PAS_chr4_0821) that was the major source of methanol-oxidizing activity in methanol-grown ...P. pastoris had been ... See full document
9
Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
... extract and Bm86-CG recombinant protein (rBm86-CG) were produced using BALB/c ...transformation in P. pastoris; 5´AOX1 primer: annealing site of primer 5´AOX1; Alpha- factor: sequence corresponding ... See full document
8
Expressão heteróloga de defensina de ervilha (pisum sativum) em pichia pastoris e avaliação da atividade biológica contra fungos de impotância agronômica
... crops in Brazil and worldwide has been severely limited in recent years by the occurrence of diseases caused by ...glycines and Macrophomina phaseolina that can generate significant losses ... See full document
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HATL5: a cell surface serine protease differentially expressed in epithelial cancers.
... serine protease domain was produced in yeast using a Pichia Expression Kit (Invitrogen, Life Technologies, Grand Island, ...serine protease sequence from the pcDNA ...tatcgcaattgg-39 ... See full document
11
Expressão e caracterização de uma protease de interesse biotecnológico clonada da...
... system and are promising for the treatment of a variety of hemostatic ...disorders. In the present study, we describe the expression of a serine protease from Crotalus durissus collilineatus ... See full document
54
Elucidation of the molecular mechanisms underlying the cytotoxic effect of recombinant frutalin in human tumor cells
... grown in minimal selective medium with 2% (w/w) glucose (Sigma), ...(Difco), and all the amino acids required for yeast growth (50 µg/mL; Sigma) except tryptophan (for wt p53, TAp63α, ΔNp63α and ... See full document
82
Expressão heteróloga de celulases de Myceliophthora heterothallica F.2.1.4 em Pichia pastoris e Escherichia coli com a caracterização e purificação das enzimas produzidas
... JAVIDPOUR, P.; PEREIRA, J.H.; GOH, E.B.; MCANDREW, R.P.; MA, S.M.; FRIEDLAND, G.D.; KEASLING, J.D.; CHHABRA, S.R.; ADAMS, P.D.; BELLER, H.R. Biochemical and structural studies of NADH-Dependent FabG used to ... See full document
35
ExpressãoCaracterizaçãoCitotóxica
... em Pichia pastoris, a purificação em cromatografia de afinidade em resina de Ni-NTA Superflow e, posteriormente, a caracterização bioquímica e funcional, pelas atividades coagulante, fibrinogenolítica e ... See full document
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Eukaryotic expression system Pichia pastoris affects the lipase catalytic properties: a monolayer study.
... back and forth over the reservoir to keep the surface pressure (p) constant, thus compensating for the substrate molecules removed from the film by enzyme ...measured in the reservoir compartment with a ... See full document
8
Produção de fragmento recombinante de anticorpo em Pichia pastoris
... Foram estudados a composição e o pH do meio de cultivo para a produção do fragmento de anticorpo (scFv) anti-LDL(-), expresso em Pichia pastoris recombinante. Os experimentos que definiram a composição e pH ... See full document
84
Nonclassical inclusion bodies in <it>Escherichia coli</it>
... context, and particularly in emerging fields such as proteomics, functional genomics and structural biology, recombinant protein production is being considered as a pivotal set of technologies that ... See full document
19
Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+
... zone, and a transition zone, as observed in Figure 1 ...ducts and comprises approximately 25% of the prostate and surrounds the ejaculatory ducts ...cells and surrounded by smooth ... See full document
68
Expressão de uma quitinase de Violaceum em Pichia Pastoris: purificação e caracterização parcial da proteína recombinante.
... 280 and 290 nm, producing emission spectra in which the wavelengths of maximum emission were 339 and 342 nm, ...residues in limited contact with ...substrates and the pH dependency of ... See full document
110
Avaliação de fitases microbianas em dietas de frangos de corte
... para ganho de peso e consumo de ração em concentração de 500 FTU/kg, em relação a fitase E. coli, com 1000 FTU/kg. Ambas as fitases reduziram teores de fósforo total e fósforo solúvel na cama. A biodisponibilidade ... See full document
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