[PDF] Top 20 A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)
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A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)
... myxoma virus and classical ...in the domestic rabbit industry, is not practiced in this ...Vaccination of captive wild rabbits against RHDV2 may provide temporary protection against infection ... See full document
8
Detection and typing of highly pathogenic porcine reproductive and respiratory syndrome virus by multiplex real-time rt-PCR.
... diverse virus strains and diagnostic samples including serum and lung samples, the new multiplex RT-qPCR showed a high diagnostic ...However, the number of used strains is limited ... See full document
9
Different methods of real-time PCR for detection of pseudorabies virus
... Aujesky Disease. In: _____. Manual of diagnostic tests and vaccines for terrestrial ...investigation of the prevalence of Aujeszky’s disease in feral swine (Sus scrofa) in ... See full document
7
Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.
... Real-time PCR has been implemented in clinical laboratories for the detection of bacterial meningitis ...burdens of disease, laboratories are required to test ... See full document
7
Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
... by the positive-strand RNA dengue virus ...cause disease in tropical and subtropical ...therefore, RT-PCR has been increasingly used in dengue diagnosis. The CDC DENV-1–4 ... See full document
15
Accurate detection and quantification of the fish viral hemorrhagic Septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay.
... issues, the present research describes and evaluates a new, accurate, fast, and highly reliable assay to diagnose and quantify ...StaRT- PCR, which is a form of competitive template ... See full document
12
Viral Genome-Linked Protein (VPg) Is Essential for Translation Initiation of Rabbit Hemorrhagic Disease Virus (RHDV).
... replicates. The amplification was con- ducted using a Mastercycler ep realplex real-time PCR system (Eppendorf, Germany) with the following program: 95°C for 10 min, followed by 40 ... See full document
13
Desenvolvimento de testes para triagem de amostras de sêmen bovino para Bovine viral diarrhea virus e Bluetongue virus utilizando RT-PCR convencional e em tempo real (qRT-PCR)
... has the largest commercial cattle herd in the world, and due to the importance of the semen and its trade, it has become necessary to develop sensitive and specific molecular tests for ... See full document
125
Detection of infectious laryngotracheitis virus by real-time PCR in naturally and experimentally infected chickens.
... infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens ... See full document
10
Development and optimisation of a group-specific real-time RT-PCR assay for the broad detection of the Simbu serogroup orthobunyaviruses
... 2017). The majority of the assays target the S segment, which has the least amount of variation (Figure 1) (Saeed et ...targeting the L segment has also been described ... See full document
112
Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR
... aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that ... See full document
8
Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses.
... series of one-step real-time qRT-PCR assays for multiplex detection of 28 viruses, which covered nearly all the important viral pathogens that cause VHFs, including ... See full document
11
Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
... leafroll disease (GLRD) is the most economically damaging and widespread viral disease of grapevine throughout the ...leafroll-associated virus (GLRaV) belonging to the ... See full document
5
TaqMan MGB probe fluorescence real-time quantitative PCR for rapid detection of Chinese Sacbrood virus.
... with the MGB groups form extremely stable duplexes with single-stranded DNA targets, and therefore allow shorter probes to be used for hybridization-based ...in the MGB region of the duplex. ... See full document
7
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples
... Argentina, 2 Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Chaco, Argentina, 3 Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon, Cayenne, French Guiana, France, 4 ... See full document
18
Development of Real Time PCR Meat detection kits and technology market transfer
... in the minor groove of the dsDNA, at the end of each PCR cycle (Navarro et ...2003). The binding of these dyes increases its fluorescence, allowing the ... See full document
132
Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR
... dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was ...reaction of raw semen in at least two sampling dates, and most of them had detectable ... See full document
3
REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... hampered the transition from research to routine use in food microbiology laboratories (Liu et ...Application of real-time PCR surmounts these shortcomings by removing the ... See full document
9
Infectious Bronchitis Virus: Detection and Vaccine Strain Differentiation by Semi-nested RT-PCR
... for the detection of IBV, followed by the discrimination of H120 vaccine strain from other IBV strains, including the M41 strain, which is genetic and antigenically closely ... See full document
8
Desenvolvimento PCR em Tempo-Real em sistema TaqMan® para detecção de rotavírus...
... de PCR em Tempo Real para diagnóstico para o gene codificador NSP5 de rotavírus bovino, um gene que apresenta uma relativamente alta conservação dentre as diferentes amostras ... See full document
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