[PDF] Top 20 RNA extraction, RT and Real-Time PCR (qPCR)
Has 10000 "RNA extraction, RT and Real-Time PCR (qPCR)" found on our website. Below are the top 20 most common "RNA extraction, RT and Real-Time PCR (qPCR)".
RNA extraction, RT and Real-Time PCR (qPCR)
... instruments, and optimized reactions allows the determination of the number of DNA molecules of a particular sequence in a sample with unprecedented accuracy and enough sensitivity to detect one single ... See full document
7
Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
... total RNA extractions from 1g of scrapings of mature stems were performed using the adsorption of nucleic acids on silica particles, grinding plant tissues in liquid nitrogen (DUBIELA et ...grapevines and ... See full document
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Circular RNAs Co-Precipitate with Extracellular Vesicles: A Possible Mechanism for circRNA Clearance.
... circRNAs and linear forms of the same mRNAs could be equally excreted by the cell in EVs, in which case we would expect that the ratios of these molecules in EVs should be similar to the ratios within the ... See full document
11
Detection and typing of highly pathogenic porcine reproductive and respiratory syndrome virus by multiplex real-time rt-PCR.
... fever and severe morbidity and mortality in pigs of all ages, emerged in swine farms all over China ...pigs and causes enormous economic losses ...several real-time reverse ... See full document
9
Canine distemper virus detection by different methods of One-Step RT-qPCR
... Step RT-qPCR assays over conventional RT-PCR and two-step qPCR detection methods, particularly for diagnosis, include their large dynamic range, ability to be scaled up for high through put ... See full document
6
Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
... singleplex and multiplex formats, which allows users to run only one test per sample and maximize use of reagent, consumables and ...format and was observed at high CTs ( ...serotypes ... See full document
15
Morphophysiological and molecular responses of sugarcane genotypes to water stress
... transpiration and stomatal conductance were significantly higher in that genotype than in the susceptible ...taller and heavier ...characterized and 8 of them were validated by ... See full document
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Key words: Biofilm formation, biosurfactant, gtfBC, Lactobacillus acidophilus, real-time RT PCR,
... gtfB and gtfC genes expression level. The expression of these genes and the production of insoluble extracellular glucans mediate the attachment of ...caries and high content of insoluble glucans in ... See full document
10
Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-PCR assays.
... vortexed, and a 100- m L volume was used for total nucleic acid extraction using the QIAamp Viral RNA Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s ...One-Step ... See full document
6
Rev. Soc. Bras. Med. Trop. vol.47 número3
... Additionally, real-time PCR is more sensitive than classical PCR, with limits of detection of ...commercial real-time platforms have become available for the detection and ... See full document
8
Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR
... Duplex real-time RT-PCR reactions either from different plants or from an individual, double infected plant, successfully detected the viruses mentioned before (GLRaV-1 not included), ... See full document
8
Rev. Soc. Bras. Med. Trop. vol.49 número3
... rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great ...evaluate and optimize a quantitative real-time reverse transcription polymerase ... See full document
7
Gene expression profiling of mitochondrial oxidative phosphorylation (OXPHOS) complex I in Friedreich ataxia (FRDA) patients.
... nDNA and mtDNA subunits of complex I and NDUFA4, a subunit from complex IV, was investigated using real time-PCR (Table ...patients and 24 controls were subjected to real ... See full document
10
Comparison of real-time PCR and conventional PCR for detection of
... 13A and 13B for kDNA of Leishmania ...66.3% and a kappa coefficient of ...IFAT and 93.7% by cnPCR using primers 13A and 13B ...primers and DNA extraction by similar methods, the ... See full document
6
Aperfeiçoamento do ensaio de quantificação do RNA mensageiro da tiroglobulina por RT-PCR em tempo real no seguimento de pacientes com carcinoma diferenciado da tiróide
... sensitivity and to hinder the interference by TgAb, several authors have developed qualitative RT-PCR assays to amplify Tg messenger RNA (TgmRNA) present in circulating thyroid cells from ... See full document
48
The flavoproteins CryD and VvdA cooperate with the white collar protein WcoA in the control of photocarotenogenesis in Fusarium fujikuroi.
... Generation and analysis of cryD overexpression in a ΔwcoA ...of PCR amplification products obtained from DNA samples of the ΔwcoA mutant SF226 and the SF226-derived cryD overexpressing strain (oe) ... See full document
22
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance
... conventional RT-PCR and real- time RT-PCR techniques has significantly reduced the processing time required to permit the detection of both the virus in the early ... See full document
6
Análise da expressão de genes em macrófagos durante uma cinética de interação com o paracoccidioides brasiliensis
... membranes and lymph ...adhesion and invasion of host cells are essentials events involved in infection and dissemination of ...macrophages and expression of genes that activate the ... See full document
87
The effect of laminin-1-doped nanoroughened implant surfaces: Gene expression and morphological evaluation
... morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit ...µg/mL) and inserted into the rabbit ...removed and subjected to morphological analysis using ... See full document
10
Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
... dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical ...virus and other flaviviruses, and an accurate Zika diagnosis is best ... See full document
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