[PDF] Top 20 Scalable passaging of adherent human pluripotent stem cells.

... a passaging solution that would perform better in multi- layer culture, we selected another non-enzymatic chelating agent, sodium citrate, and optimized its formulation to meet the critical attributes originally ... See full document

... absence of animal proteins, the inclusion of human serum albumin (HSA) and human-sourced matrix proteins makes the production process expensive and impractical for ...components of hES ... See full document

... Involving Human Subjects ...part of routine care for prostate ...streptomycin. Cells were further disaggregated by pipetting and serial filtration through cell dissociation sieves (size 40 and 80 ... See full document

... induction of cardiomyocytes from hiPSCs hiPSCs (201B6) were differentiated toward cardiomyocytes using modified-directed differentiation protocol (Method, Figure ...TNNT2-positive cells appeared at day 7–8 ... See full document

... number of passages for ‘‘convergence’’ of the aberrant hyper-methylation seems be dependent on parental cell types and their ...Disappearance of iPSCs in culture within 10 passages is occasionally ... See full document

... Neural stem (NS) cell lines have previously been established from human fetal cortex (embryonic day 50–55) ...NS cells resemble radial glia and, although maintained in adherent culture in ... See full document

... growth of hPSCs [8,9,26,27,38]. In these studies, the pluripotency of hPSCs was confirmed by teratoma and EB formation and analysis of the expression of markers specific to all three germ ... See full document

... percentage of antigen-expressing cells measured by immuno- fluorescence staining exhibited no significant difference between hiPSC-derived OPs and hESC-derived ...few cells. Contamination with other ... See full document

... for human pluripotent stem cells including human embryonic stem cells (hESCs) and human induced pluripotent stem cells ...the passaging ... See full document

... Cardiac cells experience mechanical strains with every heartbeat, ...frequencies of 1 Hz on commercial or custom built devices to engineered cardiac tissues (Gwak et ...protocol, cells were subjected ... See full document

... induced pluripotent stem cell repro- gramming protocol for use during induced pluripotent stem cell-based cell therapies in treating retinal degenerative diseases and to compare the relative ... See full document

... capacity of mammals is limited and can rarely regenerate a specific organ or tissue ...regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem ... See full document

... use of a GD iPSc model for mechanistic studies ...a human neuroglioma cell line (H4) indicating that knockdown of GCase impeded clearance of α-synuclein, these authors used GD iPSc derived ... See full document

... function of the nervous system is maintained via well-coordinated processes both in central and peripheral nervous tissues, which govern the homeostasis of organs/tis- ...Impairments of the nervous ... See full document

... analysis of the biological functions of differentially expressed genes in all 20 iPSC lines ...number of differentially expressed genes between the iPSC lines and the original cell ...lines. ... See full document

... characterization of both control and AS 30y iNs suggested that the infected fibroblasts subjected to neural direct conversion, or at least the great majority of them, did not acquired neuronal ...protocol ... See full document

... tuning of gene ...level of XPC expression is finely regulated and maintained at low background ...degradation of these proteins by the proteasome was observed ...Overexpression of XPC could be ... See full document

... EC cells using an apoptosis detection kit (In Situ Cell Death Detection Kit, Fluorescein; Roche Applied Science, Indianapolis, ...harvesting, cells were fixed in 2% paraformaldehyde in PBS for 30 min at 4 ... See full document

... these cells were subjected to the hematopoietic colony-forming cell assay, various hematopoietic CFU colonies were ...CD34+ cells are truly multipotent, with the ability to form all types of blood ... See full document

... to pluripotent stem cells. Given that expression of the DNMT3B protein isoform is down regulated faster than OCT4 protein upon spontaneous differentiation of pluripotent ... See full document