[PDF] Top 20 Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
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Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... comparison of two different computer programs, geNorm [22] and NormFinder [23], was used to select the best internal controls for normalization of gene expression studies in ... See full document
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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... analyses in Arabidopsis thaliana and soybean that show highly stable expression levels ...These reference genes include SAND family protein (SAND), protein phosphatase 2A (PP2A), ... See full document
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Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... used in the qRT-PCR analyses are presented in Table ...amplicon using Primer-BLAST ...Standard RT-PCR was performed for all the primer pairs and a single amplifica- tion ... See full document
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
... transcription-quantitative PCR (RT-qPCR) is sensitive, specific and easy to operate, and is not only preferred in quantitative gene studies, but is also considered ... See full document
8
Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology
... limited in water compared to ...levels of tol- erance and different ways to overcome flooding, including biochemical as well as morphological ...increase in reactive oxygen spe- cies (ROS) ... See full document
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... availability of the full sequence of the melon genome and multiple transcriptome data has inspired many diverse molecular biology studies performed in melons [2,19,31– ...Because ... See full document
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Importance of suitable reference gene selection for quantitative RT-PCR during ATDC5 cells chondrocyte differentiation.
... influence of reference genes on the expression profiles of target genes, cells cultured in GM, IM and CM were harvested at day 7 followed by qPCR ...data of ... See full document
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Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.
... case of endothelial cells of the blood-brain barrier (BBB) of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases ... See full document
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Gene expression profiling of mitochondrial oxidative phosphorylation (OXPHOS) complex I in Friedreich ataxia (FRDA) patients.
... quantification of the transcript levels of the genes encoding for the nDNA and mtDNA subunits of complex I and NDUFA4, a subunit from complex IV, was investigated using ... See full document
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Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... results of the present study indicate that the use of suitable reference genes for reliable gene expression evaluation by RT-qPCR should consider the type of ACL ... See full document
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Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp.
... recombination and has been identified as a molecular marker for initial stages of meiosis because it was specifically expressed during the early meiotic prophase ...found in several species such as ... See full document
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Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... estimate gene expression stability, the comparison of the different methods could be the best way to identify the best reference genes to be ...NormFinder and BestKeeper) to ... See full document
9
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
... report of a systematic evalua- tion of potential reference genes with regard to their use- fulness as normalizers in malignant glioma expression ...keeping genes, we found ... See full document
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Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... one of several members of a small family of globular proteins, and is the major building block of microtubules in almost all eukaryotic cells, exhibited the most stable ... See full document
9
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... Real time quantitative reverse transcription-PCR (qRT-PCR) is increasingly used in gene expression analysis owing to its simple, reproducible and ... See full document
10
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... the analysis of gene-expression stability and rank; the best reference gene for soybean was selected by the GeNorm application (Vandesompele et ...a gene stability ... See full document
8
Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... Gene expression analysis plays a major role in understanding of the complex regulatory net- works and identification of genes relevant to new biological processes ... See full document
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Quantitative Real Time PCR
... examined in this study. These isolates have been shown to possess high acid and bile tolerance, antioxidant activity, and ability to produce fatty acids (unpublished ...inoculum in sterilized ... See full document
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Genomic signatures predict poor outcome in undifferentiated pleomorphic sarcomas and leiomyosarcomas.
... sequences of candidate regions were obtained from the Ensembl Genome Browser website (GRCh37/hg19 Human Reference Assembly; February ...designed using the Primer-Blast online software tool ... See full document
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Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... ideal reference gene has stable expression, regardless of experimental conditions applied to the ...number of studies have shown that expression of reference ... See full document
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