[PDF] Top 20 Senescence of primary amniotic cells via oxidative DNA damage.
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Senescence of primary amniotic cells via oxidative DNA damage.
... Amnion cells were homogenized in RIPA buffer with protease inhibitors using a bullet blender (Next Advance, Averill Park, ...mg of protein were separated by SDS-gel electrophoresis (Bio- Rad, Hercules, CA) ... See full document
9
Toxic effect of silica nanoparticles on endothelial cells through DNA damage response via Chk1-dependent G2/M checkpoint.
... maintenance of ROS homeostasis depended on the respiratory chain and the membrane potential, oxidative damage may occur due to the decreasing of membrane potential ...addition, DNA ... See full document
13
DNA damage induces down-regulation of Prp19 via impairing Prp19 stability in hepatocellular carcinoma cells.
... For western blot, cells were lysed in lysis buffer (20 mM Tris- HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, and 1% Triton X- 100 with protease inhibitor), and their extracts were clarified via centrifugation. The ... See full document
6
Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.
... Cells in 60 mm culture dishes or 35 mm culture dishes were collected and lysed with RIPA solution (Beyotime). Samples were centrifuged at 10,000 g for 15 min at 4°C, and total protein concentrations determined ... See full document
16
Cell cycle control and DNA damage response of conditionally immortalized urothelial cells.
... examination of such tissue-specific effects of the bladder microenvironment on DNA damage recognition is hampered by the lack of truly appropriate urothelial ...Cultured primary ... See full document
11
Metformin (dimethyl-biguanide) induced DNA damage in mammalian cells
... variety of insulin-resistant and prediabetic states, including impaired glu- cose ...in cells of Chinese hamster ovary, CHO-K1 (chro- mosome aberrations; comet assays) and in mice (micronucleus ... See full document
6
Two 4N cell-cycle arrests contribute to cisplatin-resistance.
... loss of S-phase cells, and an accumulation of cells in a 4N ...accumulation of sub-G1 (apoptotic) cells at this 48 hr time ...percentage) of 4N arrested cells and a ... See full document
8
Protective Effect against Hydroxyl-induced DNA Damage and Antioxidant Activity of Citri reticulatae Pericarpium
... completion of radical generation, then diluted with 95% ethanol (about 1:50) so that its absorbance at 734 nm was ...aliquot of diluted ABTS + • reagent was mixed with 0.3 mL of sample ethanolic ... See full document
7
Evaluation of genotoxic and cytotoxic effects of hydroalcoholic extract of Euphorbia tirucalli (Euphorbiaceae) in cell cultures of human leukocytes
... number of leukocytes in a Neubauer chamber (dos Santos Montagner et ...loss of membrane integrity, which was indicated with trypan blue (Burow et ...measuring oxidative DNA damage, it ... See full document
12
A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
... aliquot of peripheral blood lymphocytes was added to 120 m l of ...spleen cells) were treated separately with 100 m M H 2 O 2 for 5 min, in triplicate, to ... See full document
9
CDK2 and PKA mediated-sequential phosphorylation is critical for p19INK4d function in the DNA damage response.
... regulation of protein localization provides cells with a convenient way to modulate their ...spite of its phosphorylation ...modification of T141 would be dispensable in this matter. In view ... See full document
14
J. Pediatr. (Rio J.) vol.84 número2 en v84n2a09
... The oxidative/ antioxidative balance shifted significantly to the oxidative side, because other indicators of oxidative status, TOS and OSI level, were significantly increased in infants ... See full document
6
Pea p68, a DEAD-box helicase, provides salinity stress tolerance in transgenic tobacco by reducing oxidative stress and improving photosynthesis machinery.
... overexpression of pea DNA helicase (PDH45) was shown to confer salt tolerance in tobacco and rice ...and oxidative stresses [16]. OsSUV3, a member of DEAD-box RNA helicase, has been shown to ... See full document
13
DNA damage in the kidney tissue cells of the fish Rhamdia quelen after
... the DNA damage seen in Hoplias malabaricus subjected to in- organic lead contamination by intraperitoneal ...demonstrated DNA damage in this tissue by monitoring the Santa Marta and Camacho ... See full document
8
Actividade moduladora de extracto de Ginkgo biloba no ciclo celular de Saccharomyces cerevisiae e capacidade de reparação de danos de DNA em células sob stresse replicativo
... recognizes DNA damage caused by UV radiation, besides the participation in cell cycle ...cerevisiae cells was tested. For this, yeast cell cultures of BY4741 and the UV-sensitive mutants, rad4 ... See full document
46
Clinical symptoms in fibromyalgia are better associated to lipid peroxidation levels in blood mononuclear cells rather than in plasma.
... register of the Sevillian Fibromyalgia Association (AFIBROSE) and 45 healthy matched controls were enrolled into our ...approval of the local ethical committee were ...diagnosed of FM in the last 2–3 ... See full document
6
Lycopene and ß-carotene protect in vivo iron-induced oxidative stress damage in rat prostate
... mediate oxidative stress (11-14). In fact, a high production of free radicals occurs in animal models of iron overload ...development of hepatocellular carcinoma ...ducer of lipid ... See full document
8
Improved mitochondrial function underlies the protective effect of pirfenidone against tubulointerstitial fibrosis in 5/6 nephrectomized rats.
... effect of pirfenidone on mitochondrial function was determined using the mitochondrial membrane potential (MMP), mitochondrial DNA (mtDNA) copy number and ATP ...HK2 cells reduced MMP, which is an ... See full document
15
Resveratrol Induced Premature Senescence Is Associated with DNA Damage Mediated SIRT1 and SIRT2 Down-Regulation.
... [r] ... See full document
20
Estudos de reparo de DNA por excisão de nucleotídeos em lesões oxidativas em células...
... O que diferencias essas duas sub-vias do NER é o passo do reconhecimento da lesão. No TCR sugere-se que a RNApol II é bloqueada frente a lesão e este é o passo principal para o direcionamento das proteínas de reparo para ... See full document
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