Cell lysis
A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis.
... Third, the assay is very sensitive and is able to detect molecules with anti-mycobacterial activity at concentrations well below MIC. For example, a number of the molecules identified in our screen of a ChemBridge ...
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Inactivation of the Autolysis-Related Genes lrgB and yycI in Staphylococcus aureus Increases Cell Lysis-Dependent eDNA Release and Enhances Biofilm Development In Vitro and In Vivo.
... Staphylococcus aureus ica-independent biofilms are multifactorial in nature, and various bacterial proteins have been associated with biofilm development, including fibronectin- binding proteins A and B, protein A, ...
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Clinical-grade oncolytic adenovirus purification using polysorbate 20 as an alternative for cell lysis
... host cell proteins and DNA are removed, while maintaining a low viral/infectious particles ratio (bellow ...scalability. Cell lysis for large-scale processes is usually achieved by the addition of ...
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Fractalkine expression induces endothelial progenitor cell lysis by natural killer cells.
... (A) Cytotoxicity assay performed using PBMC effector cells isolated from 7 independent blood donors as described in Methods. Each independent assay was performed in triplicate (n = 7) and analysed using an effector: ...
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Colon stem cell and crypt dynamics exposed by cell lineage reconstruction.
... tubes. Cell lysis, 10 min at 30 uC, was done by adding to each tube ...l cell lysis solution (600 mM KOH, 10 mM EDTA, 100 mM dithiothreitol (DTT)), followed by the addition of ...
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Uric acid and the prediction models of tumor lysis syndrome in AML.
... Tumor lysis syndrome (TLS) is a medical emergency, a consequence of cell lysis and the rapid release of intracellular contents into the blood stream and the potential for subsequent multiple organ ...
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Lysis strategy of Streptococcus pneumoniae bacteriophages : mechanisms and host implications
... for cell lysis following death from energy poisons ...understood. Cell depolarization may induce structural and spatial changes in the membrane [17,50,51] leading to LytA activation, probably by ...
188
Functional analysis of lysB gene from the lysis module of mycobacteriophage Ms6
... in cell lysis for enzyme therapy against pathogenic ...of lysis and allows the phage endolysin to cleave its ...to cell lysis and release of the progeny phage (Young et ...bacterial ...
146
Insights into cell wall synthesis and cell division in Staphylococcus aureus
... of cell wall synthesis by cross-linking the stem peptides of different glycan chains, essential for the overall structure and integrity of the bacterial cell ...the cell wall ...consequently ...
222
Characterization and functional analysis of the Gp1 accessory lysis protein in Mycobacterium smegmatis infection by the Mycobacteriophage Ms6
... family Pham66-1 (Payne et al., 2009; Hatfull et al., 2010), although this group of enzymes is not restricted to phages that infect mycobacteria (Hatfull et al., 2010). The Lysin A (LysA) family of proteins appears to be ...
229
Mycobacteriophage-mediated lysis : the role of LysB proteins
... that lysis of the bacterial host is the last event of dsDNA phage lytic cycle, so that the new synthesized phage particles may be released into the environment and infect new available ...bacterial cell ...
215
Giant cell glioblastoma
... giant cell glio- blastoma is not encountered in everyday practice and it probably comes as an unexpected diagnosis most of the ...giant cell glioblastoma managed at the authors’ institution is reported and ...
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Sickle cell disease
... A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS, OSTEOCYTES and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydoxyapatite ...
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Determinants of cell-to-cell variability in protein kinase signaling.
... the cell may be globally correlated, possibly due to fluctuations in RNA polymerase and/or ribosome copy numbers ...our cell-to-cell variability simulations, we made a conservative assumption and ...
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Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures
... mean cell volume and number of adipocytes yield very close figures, with a small difference in cell size over vacuolar fat ...which cell volume is upwards of 75% of its volume, the 50-fold lower ...
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Monitoring cell-cell contacts in vivo in transgenic animals
... controls cell fate during development through cell-cell interactions (Artavanis-Tsakonas and Muskavitch, ...the cell membrane (Brou et ...of cell fate-related genes (Struhl and Adachi, ...
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Tetherin restricts productive HIV-1 cell-to-cell transmission.
... The cell-to cell viral fusion assay was adapted from a cell-free virion fusion assay [40]. HeLa donor cells were cotransfected with Figure 5. Tetherin promotes transfer of large viral patches and ...
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Cell to cell communication in plants: ions take charge
... As early as 1905, Mereschkowsky stated that chloroplasts might have evolved from free-living cyanobacteria, which were engulfed by a protozoan host cell (Mereschkowsky, 1905). The adaptation of the ancestral ...
66
Mol Cell Probes
... Besides confirming the usefulness of the Bi-PASA in polymorphism analysis, the data presented provide valuable information on TLR polymorphisms in the Portuguese population that can be us[r] ...
5
Langerhans cell histiocytosis
... H and-SchU ller-C hristian, L etterer-Siw e and. eosinophilic granulom a w ere the first clinical descriptions. L ater on, they w ere nam ed H istiocytosis X , w here X stood for etiology unknow n. In 1985 the ...
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