directed evolution
In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification.
... rected evolution of proteins containing non-canonical amino ...and directed evolution may prove a fruitful manner by which to identify residues amenable to these next generation protein modification ...
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Directed evolution of activation-induced cytidine deaminase (AID) in its natural environment
... a directed evolution experiment of AID to isolate mutants with enhanced ...natural evolution in culture, at the protein level, through the reiteration of two main events: Diversification & ...
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Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity.
... conducted directed evolution for this area using epPCR and T7 phage display technology to obtain polypeptide P1, which could block the LBP site for binding to ...
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Development of novel bacterial enzymes for medical diagnosis through directed evolution
... work, directed evolution (DE) methodologies were applied to improve the first identified bacterial P2Ox, from Pseudoarthrobacter siccitolerans, AsP2Ox, in its specificity and activity for ...
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Improving a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans through directed evolution
... yields. Directed evolution has proven to be a powerful approach to improve enzyme efficiency and robustness required for biotechnological ...of directed evolution was performed, namely ...
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Directed evolution of a model primordial enzyme provides insights into the development of the genetic code.
... While the global structure seems largely unperturbed in all of the simplified CMs, the MD simulations revealed how local interactions that are perturbed in the simplified CMs were restored during directed ...
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Improving kinetic or thermodynamic stability of an azoreductase by directed evolution
... by directed evolution [50] while A281E and V221D mutations inhibited protein aggregation and facilitated refolding of the catalytic active conformation of a Candida antartica lipase ...
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Directed evolution of lichenicidin
... Bliβ, that is produced by several strains of Bacillus licheniformis. These peptides act synergistically in order to inhibit the growth of other Gram positive bacteria. Due to their an[r] ...
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Directed Evolution of a Highly Specific FN3 Monobody to the SH3 Domain of Human Lyn Tyrosine Kinase.
... Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations and quantities of individual proteins. To generate high-quality affinity reagents for human Lyn tyrosine kinase, a ...
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Directed evolution of model-driven spreadsheets
... spreadsheet evolution were already proposed [Cunha et ...this evolution had to be done manually, and a human operator had to apply successive transformations to the spreadsheet model until it reached the ...
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Inferring shape evolution.
... and evolution of a morphing process involve mapping the shape state (a vector in the phase space) considering as state variables its x- and y- coordinates parameterized along time, therefore defining a shape ...
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Science and evolution
... promoting evolution (Dunk et ...of evolution and science fundaments in admission policies for biology pro- fessionals, particularly teachers (Larkin and Perry-Ryder, 2015; see Rutledge and Warden, 2000 for ...
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Tele-Enfermeiro Evolution
... were: nursing focus or diagnosis (phrase on the health status of a patient) and nursing intervention (addressed to the patient). The three action targets (individuals, families and community) were also discussed and it ...
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Web telephony evolution
... The evolution of smartphones, with more processing power and more user-focused features, as brought an ever- increasing interest in mobile applications, with the creation of marketplaces designed to facilitate ...
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Gaze-directed gameplay in first person computer games
... Identifying the lack of studies that try to pinpoint the advantages and disad- vantages of eye tracking integration in games, as well as the discrepant results obtained in different stud[r] ...
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Analytical approach to directed sandpile models on the Apollonian network
... We investigate a set of directed sandpile models on the Apollonian network, which are inspired by the work of Dhar and Ramaswamy 关Phys. Rev. Lett. 63, 1659 共1989兲兴 on Euclidian lattices. They are characterized by ...
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Generating Property-Directed Potential Invariants By Backward Analysis
... the use of formal methods for the verification and validation of safety critical flight control software and allow their use in development processes. Successful examples of industrial scale formal methods ap- plications ...
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Infants distinguish antisocial actions directed towards fair and unfair agents.
... Three experiments provide evidence of an incipient sense of fairness in preverbal infants. Ten-month-old infants were shown cartoon videos with two agents, the ‘donors’, who distributed resources to two identical ...
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New methodologies for directed functional connectivity applied to brain neuroimaging research
... Since a major part of this work focuses on testing and benchmarking connectivity metrics in controlled simulations, an initial study was performed on known generative models, with varying degrees of biophysical fidelity, ...
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Directed mutagenesis affects recombination in Azospirillum brasilense nif genes
... Such plasmids have been used as suicide vehicles to deliver transposons in Azospirillum and the transposon mutagenesis technique has been efficient in generating dif- ferent Nif- mutants[r] ...
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