Functional Studies
Structural-functional studies of carboxylic acid transporters : novel tools in production optimization using industrial microbes
... no functional study was performed in those organisms (Soares-Silva et ...these studies LacY crystallographic structure was used to obtain a hypothetical 3D structure of Jen1p and posteriorly a refined model ...
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Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni
... Heterologous expression experiments have been performed an alternative approach to characterize schistosome genes. In the present paper, we described for the first time the use of C. elegans as an alternative ...
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In silico analysis of missense mutations as a first step in functional studies: examples from two sphingolipidoses
... to functional studies, we have selected 23 missense mutations distributed in different domains of two lysosomal enzymes, to be studied by in silico ...of functional studies, regarding the ...
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Molecular evolution of the infrared sensory gene TRPA1 in snakes and implications for functional studies.
... TRPA1 is a calcium ion channel protein recently identified as the infrared receptor in pit organ-containing snakes. Therefore, understanding the molecular evolution of TRPA1 may help to illuminate the origin of ‘‘heat ...
11
Functional Studies on Magnetic Resonance
... Some studies have demonstrated that ASL holds several advantages when compared with BOLD, namely the fact of being more reproducible and allowing perfusion quantification ...
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Mutagenesis and functional studies with succinate dehydrogenase inhibitors in the wheat pathogen Mycosphaerella graminicola.
... The primary aim of this study was to understand possible target site resistance mechanisms to a range of newly introduced subclasses of carboxamides in M. graminicola by exploring the impact of target mutations on ...
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New insights into HIV-1 restriction - functional studies on 90K orthologues and endogenous SERINC5
... reasonable to speculate that IFN-α might regulate partially the quality of SERINC5 mRNA transcripts. Post-transcriptional regulation of mRNAs is driven by RNA-binding proteins (RBPs), which dictate almost all the steps ...
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Structural and Functional Studies of a Bothropic Myotoxin Complexed to Rosmarinic Acid: New Insights into Lys49-PLA(2) Inhibition
... dichroism studies exclude the proteolytic degradation of the toxin as a potential mechanism involved in the inhibition of myotoxic and inflamma- tory activities of Lys49-PLA 2 s from Bothrops jararacussu venom ...
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Structural and functional studies of PpcA: a key protein in the electron transfer pathways of Geobacter sulfurreducens
... thermodynamic studies because these spectra allow to follow the behavior of representative heme methyl signals through the oxidation of the proteins at different pH ...and functional properties of each ...
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The Leishmania TDR1 family-related gene: functional studies and evaluation of TDR1 recombinant protein as a vaccine agaist leishmaniasis
... Leishmania have efficiently developed measures to survive in the sand fly and also inside the mammalian host, where the conditions are hostile. Among the different mechanisms is the secretion/excretion or release of ...
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Evaluation of silicon nitride as a substrate for culture of PC12 cells: an interfacial model for functional studies in neurons.
... Generation of the stably transfected reporter gene cell line. PC12 cells were used as host cells in transfection experiments. Transfection was carried out using an expression vector encoding the DsRed2 fluorescent ...
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Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase.
... This work reports the biochemical and functional analysis of the Burkholderia cenocepacia J2315 bceN gene, encoding a protein with GDP-D-mannose 4,6-dehydratase enzyme activity (E.C.4.2.1.47). Data presented ...
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Structural and Functional Studies of H. seropedicae RecA Protein - Insights into the Polymerization of RecA Protein as Nucleoprotein Filament.
... the functional and structural characterization of the Herbaspirillum seropedicae RecA protein ...Our functional and structural analyses pro- vide insight into the molecular mechanisms of polymerization of ...
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Conformational and functional studies of gomesin analogues by CD, EPR and fluorescence spectroscopies
... structural studies of peptides, TOAC has also been used advantageously to monitor peptide chain assembly in polymer backbones ...and functional properties of several gomesin ...
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Structural and functional studies of FKHR-PAX3, a reciprocal fusion gene of the t(2;13) chromosomal translocation in alveolar rhabdomyosarcoma.
... Because high levels of PAX3-FKHR transcript and protein are detected in all t(2;13)-positive ARMS tumors and tumor- derived cell lines, and continuous expression of PAX3-FKHR is critical for maintaining ARMS phenotypes ...
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Application of proteomics and cytomics in human neutrophils functional studies
... the functional proteomics of neutrophils; that is, the mobilization of these proteins from one compartment to another will determine the phenotype of the ...
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MsmX as model for functional studies of Multitask ATPases from pathogenic bacteria
... Recent studies, performed in our laboratory demonstrated that MsmX ATPase from Bacillus subtilis interacts with several distinct ABC sugar importers thus, unlike other NBDs MsmX was shown to be multitask serving ...
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Functional studies of genetic variants of unknown significance
... There are several functional assays, but in this study we only four of them: TUNEL assay, Caspases activity assay, H2Ax assay and Comet assay. All of them showed some differences in all samples that may indicate ...
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Functional studies on BolA and related genes:
... structurally more closely related OsmC) (Huynen et al., 2005). In addition, strong phylogenetic connection is observed for genes encoding BolA and monothiol glutathioneredoxins as they are genome co-occurrent: either ...
246
Structural and functional studies on human enzymes involved in hydrogen sulfide breakdown
... Although the crystallographic structure of human Rhodanese has not yet been reported, Rhod is an enzyme very well functionally studied. In early days, the only known role for this enzyme was the detoxification of ...
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