Gene delivery
Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment.
... In the second engineering step we eliminated use of the natural NiV receptors ephrin-B2 and ephrin-B3 by introducing point mutations into the GcΔ34 EpCAM protein. For identifying the most effective mutations, we relied ...
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FUNCTIONALIZATION OF DENDRIMERS FOR IMPROVED GENE DELIVERY TO MESENCHYMAL STEM CELLS
... as gene delivery ...the gene delivery ...(BMP-2). Gene expression in mesenchymal stem cells (MSCs), a cell type with relevance in the regenerative medicine clinical context, was ...
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Characterization of Ku70(2)-NLS as bipartite nuclear localization sequence for non-viral gene delivery.
... Non-viral gene delivery is limited by the low endosomal escape after cellular uptake and the low translocation of DNA into the nucleus [3,4]. It has been shown that the endosomal escape could be improved by ...
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Evaluation and optimization of chitosan derivatives-based gene delivery system via kidney epithelial cells
... FITC-dextran (FD) is primarily used for studying permeability and transport in cells and tissues. The advantages of FD are that just like nucleic acids. It has negative charge and because of its fluorescence part, it can ...
10
Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA
... of gene therapy is replacing diseased genes with new healthy ...proper gene delivery system is a very important issue when considering gene therapy because naked plasmid DNA (pDNA) is rapidly ...
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Alkyl cross-linked low molecular weight polypropyleneimine dendrimers as efficient gene delivery vectors
... Objective(s): In recent years, polypropyleneimine (PPI) dendrimers have attracted great interest as non- viral gene delivery systems because of their attractive features including highly branched ...
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Gene delivery using dendrimerpDNA complexes immobilized in electrospun fibers using the layer-by-layer technique
... Tissue engineering is an important branch of regenerative medicine that uses cells, materials (scaffolds), and suitable biochemical and physicochemical factors to improve or replace specific biological functions. In ...
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Effective in vitro gene delivery to murine cancerous brain cells using carbon nanotube-polyethylenimine conjugates
... Once carbon nanotube (CNT) was discovered by Iijima [1] numerous range of studies has focused on their unique properties. Aside from their chemical properties, their nanoscale size, extreme flexibility, high tensile ...
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Sonic Hedgehog gene delivery to the rodent heart promotes angiogenesis via iNOS/netrin-1/PKC pathway.
... cells, gene delivery of iNOS to endothelial cells is protective for the cells via NO release without an influence on their proliferation ...1 delivery to the heart (group-4), the number of blood ...
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Adiabatic Differential Scanning Calorimetric Study of Divalent Cation Induced DNA - DPPC Liposome Formulation Compacted for Gene Delivery
... Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper ...
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Combining hyaluronic acid with chitosan enhances gene delivery
... low gene transfer efficiency of chitosan-DNA polyplexes is a consequence of their high stability and consequent slow DNA ...their gene transfection efficiency on both HEK293 and retinal pigment epithelial ...
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NANO-STRUCTURES FOR NON-VIRAL GENE DELIVERY FOR CANCER THERAPY: A REVIEW
... nonviral delivery systems in the extracellular compartments, such as intercellular or intravascular spaces, is related to the chemical stability of the DNA and the physical stability of the delivery ...for ...
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Development of new hybrid gene delivery carriers
... Gene therapy has gained increased attention over the last decades due to the possibility to treat a disease at its routes. Several vehicles intended to carry and deliver a functional copy of the deficient ...
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Sustained gene expression in the retina by improved episomal vectors
... with gene therapy—low levels of transgene expression, vector loss during mitosis, and gene silencing—need to be ...ocular gene therapy is an important tool for the treatment of several diseases, ...
7
Enhancement of non-viral transfection efficiency with nuclear localization signal peptides
... successful gene therapy vectors today are viruses-based gene delivery systems which provide high transduction effectiveness of the gene of interest to the target cells and superior levels of ...
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Affinity purification and delivery of a p53-encoding plasmid DNA for gene mediated cancer therapy
... promote gene transfer, non-viral systems such as nanoparticles have arisen as efficient and safe delivery ...effective gene delivery nanocomplex should provide an appropriate balance between ...
194
Braz. J. Pharm. Sci. vol.53 número3
... Huang, 2002). Each of these vectors demonstrates its own advantages and drawbacks (Cusack, Tanabe, 2002). Many deiciencies in several areas including the induction of host inlammatory and immune response are reported for ...
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Multifunctional nanocarriers for lung drug delivery
... systemic delivery has reached the market, despite those being the drugs focusing most of the attention within the context of systemic delivery mediated by ...subcutaneous delivery (1 IU/kg), but ...
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Chitosan Nanoparticles as Drug Delivery Systems
... (DOX) delivery for cancer ...drug delivery, cancer diagnostics and treatment. The DOX delivery studies indicate that the drug release depends on pH and in the first 10-20 hours the majority of drug ...
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J. bras. pneumol. vol.41 número5
... be paid to the adverse effects of high (> 500 mL) tidal volume in patients with obstructive lung disease, given that it can worsen dynamic hyperinlation or cause barotrauma. According to the authors of an in vitro ...
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