High-Resolution Melting (HRM)
Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis
... Monofloral honeys (such as, lavender honey) are highly appreciated by the consumers due to their flavour, taste and properties. However, since they are considered prime products, they are often targets of adulteration. ...
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High resolution melting : improvements in the genetic diagnosis of hypertrophic cardiomyopathy in a Portuguese cohort
... [18,24]. High Resolution Melting (HRM) is a high throughput gene variation scanning technique that relies on the differential melting properties of sequences that vary in at least one ...
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Unraveling Host-Vector-Arbovirus Interactions by Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis in a Kenyan Wildlife-Livestock Interface.
... on high resolution melting (HRM) analyses of cyt b PCR products is more ...This resolution of vertebrate host species was only possible by comparing profiles of both cyt b and 16S markers, as ...
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Reliable discrimination of 10 ungulate species using high resolution melting analysis of faecal DNA.
... A high resolution melting (HRM) assay, targeting a fragment of the 12S rRNA gene, was ...different melting behaviour for each ...enables high-throughput and cost-effective species ...
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Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.
... ‘high resolution melting analysis or high-resolution fluorescent melting curve analyses or High-resolution amplicon melting analysis’, ‘HRM or HRMA or ...
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Combined mutation and rearrangement screening by quantitative PCR high-resolution melting: is it relevant for hereditary recurrent Fever genes?
... denaturing high performance chromatography, HRM, High Resolution Melting analysis) that only allow for the identification of micro- ...
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Genetic diagnosis of hypertrophic cardiomyopathy using mass spectrometry DNA arrays and high resolution melting
... two high-throughput techniques – mass spectrometry genotyping (MSG) and high resolution melting (HRM) – is an encouraging new strategy for HCM diagno- ...
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High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.
... the High Resolution Melting (HRM) dissociation profiles of two amplicons using real time polymerase chain reaction (real-time PCR) targeting heat-shock protein 70 coding gene (hsp70) revealed ...
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Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting
... High-resolution melting (HRM) analysis can be used to distinguish DNA sequences with slight differences in the melting curve ...with high accuracy (Ghorashi et ...with high ...
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Rapid detection of the H275Y oseltamivir resistance mutation in influenza A/H1N1 2009 by single base pair RT-PCR and high-resolution melting.
... We designed primers specific for influenza A/H1N1 2009 based on sequences of the NA gene downloaded from the National Center of Biotechnology Information from September 2009. A novel strategy of only amplifying a single ...
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Real-Time PCR, High Resolution Melting - aplicações forenses
... Também em 2011 Fregel et al, realizaram dois ensaios TaqMan multiplex PCR em tempo real para a quantificação de nDNA e mtDNA em amostras degradadas ou limitadas, tendo intro[r] ...
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Deteção de mutações nos genes JAK2 e MPL por high resolution melting
... Atualmente, estão descritas uma variedade de técnicas moleculares altamente sensíveis para a deteção destas mutações, em particular para a mutação V617F. Estas técnicas incluem PCR alelo específico (AS-PCR) ou ARMS ...
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Detection of somatic mutations by high-resolution DNA melting (HRM) analysis in multiple cancers.
... progression. High resolution melting (HRM) curve analysis represents a fast, post-PCR high-throughput method for scanning somatic sequence alterations in target ...
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Detecting the atmosphere of exoplanets using high resolution spectroscopy
... of equation ( 1.33 ), α increases with the distance to the star and it is larger to higher planet- star mass ratios and shorter distances. This technique is more sensitive to high mass planets with long orbital ...
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GLASS MELTING PHENOMENA, THEIR ORDERING AND MELTING SPACE UTILISATION
... consequently high and only a small part of the volume is usable for accomplishing the melting ...the melting processes increases and this leads to an increase of the space utilization and ...
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Estimation of cell viability with high resolution growth curves
... The plating method was only used in the present study as a control. Although it provided good results, estimating cell viability through this method is time-consuming, labour intensive and it presents high ...
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Environmental inversion using high-resolution matched-field processing
... specifically, high-resolution processors, are proposed for their increased ability in attenuating ...the high- resolution processors were unable to outperform the Bartlett ...the high- ...
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High resolution GPR applied to the “Capela Pombo”
... much high density, also is important to remember, this interpre- tation base in 1 field test, to have a better interpretation is necessary combine 2 or more geophysics ...
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Meshless Difference Methods with Adaptation and High Resolution
... There is a clear advantage in using gridless methods for these problems in that a suitably chosen set of nodes can deform and move as the boundaries and interfaces change. This also means that more nodes can be added at ...
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Surface high-resolution temperature forecast in southern Italy
... Abstract. Since June 2008, 1-h temperature forecasts for the Calabria region (Southern Italy) are issued at 2.5 km horizontal resolution at CRATI/ISAC-CNR. Forecasts are available online at http://meteo.crati.it/ ...
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