Reference genes
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... determine reference genes have been conducted in plants grown in pots (SILVA et ...used reference genes are: 25S ribosomal ribonucleic acid (rRNA), glyceraldehyde 3-phosphate dehydrogenase ...
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Identification of reference genes for relative quantification of circulating microRNAs in bovine serum.
... candidate genes with M ...of reference genes required for accurate ...n reference genes are sufficient as internal ...two reference miRNAs (miR-127 and miR-93) would be ade- ...
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
... reference genes. Some studies suggest that GeNorm might select ref- erence genes of similar expression by a pairwise approach but the selected genes might not be the most stable genes, ...
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Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut
... stable genes by GeNorm, NormFinder, and BestKeeper, respec- tively (Table ...ranked genes, as they were also classified as good reference genes by GeNorm (first and second posi- tions); ...
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Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp.
... candidate genes to examine their suitability as an internal reference ...as reference genes, the Dmc1 exhibited similar profile as previously reported with the highest expression levels found ...
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Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... best reference gene for soybean was selected by the GeNorm application (Vandesompele et ...stable genes, by calculating the mean pairwise variation between a particular gene and all others used in an ...
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An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR
... the reference genes available for common beans, this work does not intend to confirm information already described in the literature, but rather intends to provide additional guidance on working with common ...
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Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).
... with reference genes that are uniformly expressed under experimental ...of reference genes for Lupinus angustifolius (narrow-leafed lupin, a significant grain legume crop) using a selection of ...
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Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... of reference genes is required for transcript level normalization under different conditions in ...used reference genes, ACT, EF1α, α-TUB, β-TUB, GAPDH, CYP2, UBQ10, and 18S ...superior ...
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Identification of Suitable Reference Genes for Gene Expression Studies in Tendons from Patients with Rotator Cuff Tear
... suitable reference genes for data ...of reference genes for the comparison between CC and PC samples was HPRT1 + TBP + ...four reference genes (HPRT1 + TBP + ACTB + B2M), showed ...
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Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues.
... suitable reference genes for gene expression studies in EC tissue samples, we carried out a Medline search using the MeSH terms ‘‘endometrial cancer’’ and ‘‘real-time ...following reference ...
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Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean.
... these genes were different with the use of different reference genes (data not ...(UKN2) reference genes under viral infection and stresses of darking, salinity and drought, with the ...
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Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
... with reference genes is necessary, in order to obtain reliable relative expression data in re- sponse to gastrointestinal nematode ...three reference genes, ...ideal reference gene, ...
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Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
... optimal reference control ...endogenous reference genes for use in studies testing strawberry- pathogen ...strawberry reference genes were tested against different tissues, strawberry ...
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Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas)
... housekeeping genes (HKGs) as reference genes to obtain correct ...appropriate reference genes in relative quantification in gene expression ...of reference gene selection, which ...
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Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.
... with reference genes of honey bee ...the genes analyzed in ...best reference genes in ...of genes act and GAPDH were too unstable to be used for normalization in ...a ...
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Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.
... candidate reference genes in RT-qPCR, the relative expression level of one papaya fruit gene, CpaEXY1, was investigated in two papaya cultivar fruit during postharvest ripening, using one or two of most ...
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Selection and evaluation of reference genes for analysis of mouse (Mus musculus) sex-dimorphic brain development
... three genes of interest, Sdha and Pgk1 oligonucleotide primers were used at ...the reference genes Actb and RpL37 were ...stage-specific reference genes ...ranked genes were ...
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Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... target genes across different ...several reference genes should serve as the internal control to normalize and monitor the expression variation between samples and reactions ...these reference ...
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Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... the reference genes a-Tubulin, TBP and AK in comparison to ...stable reference genes showed a similar trend in resistant or susceptible strain, but when HSP22 was used as the reference ...
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