Reverse transcription- polymerase chain reaction
Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut
... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is currently the most sensitive techni- que for quantification of low abundance transcripts, and at the same time ...
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Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples
... as reverse transcription-polymerase chain reaction (RT-PCR) have been shown to be useful in detecting res- piratory viruses in a clinical setting (Dayan et ...
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Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
... improved reverse transcription-polymerase chain reaction (RT-PCR) protocol for universal detection of flaviviruses by using semi-nested primers that discriminate between DENV serotypes ...
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Detection of Brazilian Hantavirus by Reverse Transcription Polymerase Chain Reaction Amplification of N Gene in Patients with Hantavirus Cardiopulmonary Syndrome
... nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using prim- ers selected to match high homology regions of hantavirus genomes detected from the ...
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Dengue Virus Detection by Using Reverse Transcription- Polymerase Chain Reaction in Saliva and Progeny of Experimentally Infected Aedes albopictus from Brazil
... and reverse transcriptase-PCR am- plification (RT-PCR) - Viral RNA was extracted from the supernatant of infected cells by using the QIAmp Viral Mini Kit (Qiagen, ...
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Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
... of reverse transcription-polymerase chain reaction (RT-PCR) and sequence analyses, RVA strains are clas- sified into the G and P genotypes, which represent the two type-specific outer ...
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Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil
... a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA ...
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Arq. NeuroPsiquiatr. vol.74 número10
... for polymerase chain reaction ...to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses ...
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Abnormal apoptosis of trophoblastic cells is related to the up-regulation of CYP11A gene in placenta of preeclampsia patients.
... placenta; reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine CYP11A expression at mRNA and protein levels in patients with severe ...
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Bautista-Gómezb, Raúl Fajardo Mu ˜noza, Israel Quijano Hernándezc
... used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were ...
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Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
... ND is a serious threat to the poultry industry [16]. The rate of mortality and morbidity of poultry in unvaccinated flock [2] varies from 90-100%, depending upon the strain of ND virus [10]. The outbreaks of ND are ...
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Rev. Soc. Bras. Med. Trop. vol.46 número2
... by reverse transcription-polymerase chain reaction (RT-PCR), the amplicon sequence of DENV-4 was analyzed, and these indings were corroborated with the detection of DENV-4 in clinical ...
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Rev. Soc. Bras. Med. Trop. vol.47 número3
... on polymerase chain reaction (PCR) targeting the HCV 5’ untranslated region ...nested reverse transcription-polymerase chain reaction (RT-PCR) and restriction ...
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Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
... trauma induces keratinocytes to produce basic fibroblastic growth factor, which activates melanocyte membrane receptors for tyrosinase KIT-Kinase and, thereafter, mRNA nuclear transcription in dormant melanocyte. ...
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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
... Odanacatib (ODN) is a selective inhibitor of cathepsin K. The cysteine protease cathepsin K has been implicated in cardiac hypertrophy. Resistine is an adipokine which is identified to promote cardiac hypertrophy. Here, ...
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... vitro transcription - A PCR was performed to am- plify the 222 nucleotides of VSV inserted into the plas- mid using Taq DNA polymerase (Invitrogen, Waltham, MA, USA), 5′-end primer containing T7 promoter ...
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J. Appl. Oral Sci. vol.12 número1
... “Reverse transcription and polymerase chain reaction: principles and applications in dentistry”, which offers an interesting and exciting analysis of the potential that molecular ...
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Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, Southern Brazil
... study, reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect the presence of HEV in swine feces and in effluents from slurry lagoons in farms located in the ...
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Pancytopenia during tyrosine kinase inhibitor treatment–coexistence of chronic myeloid leukemia and visceral leishmaniasis : a case report
... He was referred to our Department of Hematology, at our university hospital, where a bone marrow aspiration and biopsy were performed. Microscopic examination showed a left-shifted granulopoiesis, and the Ph + was found ...
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