Substrate binding
Probing the acceptor substrate binding site of Trypanosoma cruzi trans-sialidase with systematically modified substrates and glycoside libraries
... acceptor substrate binding ...TcTS binding and turnover, which highlights the potential of ‘internal’ 6-substituted galactose residues to serve as TcTS acceptor ...
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Computational of the substrate binding mode in III oxidase
... protein, binding in this conformation is almost completely due to interactions with only one of the substrate carboxylate ...The substrate partially exits the binding region, and the only ...
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Catalytic and non-catalytic roles for the mono-ADP-ribosyltransferase Arr in the mycobacterial DNA damage response.
... These microarray experiments compared three strains: M. smegmatis site(+), Darr site(+), and Darr+Flag-Arr site(+). We found numerous transcripts that were differentially expressed between Darr site(+) and wild type ...
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Homology modeling of human γ-butyric acid transporters and the binding of pro-drugs 5-aminolevulinic acid and methyl aminolevulinic acid used in photodynamic therapy.
... central substrate binding pocket in the outward-open homology models varied considerably (Figure ...putative substrate binding site was highly positive in nature, the corresponding areas in ...
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Crystal structure of cathepsin X: a flip-flop of the ring of His23 allows carboxy-monopeptidase and carboxy-dipeptidase activity of the protease
... S1′ substrate binding sites ...S2′ binding site, as has already been suggested ...‘primed’ substrate binding sites S1′ and S2′ as well as reach into the S2 binding pocket, in ...
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Structural Insight of a Trimodular Halophilic Cellulase with a Family 46 Carbohydrate-Binding Module.
... the binding of polysaccharide substrate, the CelB mutants (H103Q, W156A, W229A, W476A) were used to perform the affinity gel electrophoresis ...the binding of the ...the substrate CMC makes it ...
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The interactions in the carboxyl terminus of human 4-hydroxyphenylpyruvate dioxygenase are critical to mediate the conformation of the final helix and the tail to shield the active site for catalysis.
... putative substrate binding site, suggesting the interactions provided by Q375 to hold the terminal helix and the tail in proper position are critical for isolating the active site from solvent during ...
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The role of the methyltransferase domain of bifunctional restriction enzyme RM.BpuSI in cleavage activity.
... that substrate binding and catalysis are considered simultaneously, assuming the rate of product release is the same for all ...cleavage substrate and the unmodified cleavage product (Figure ...
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Investigation of the interaction of naringin palmitate with bovine serum albumin: spectroscopic analysis and molecular docking.
... likely substrate binding modes were obtained by extracting the lowest energy conformation from the largest cluster ...of binding sites for naringin ...docking binding sites of naringin, there ...
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Insights into the biochemical strategies of adaptation to heat stress in the hyperthermophilic archaeon Pyrococcus furiosus
... activity (Fig. 4.11). These aspartate residues are part of the hydrophilic active site cavity and are close to the magnesium ion. Moreover, mutations of consensus residues Ala364 and Arg365 to methionine caused a ...
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Modeling and dynamics of the inward-facing state of a Na+/Cl- dependent neurotransmitter transporter homologue.
... the substrate was pulled along the EC and IC directions to investigate the substrate transport pathways ...second substrate binding site (S2) which was supported by multiple experimental ...
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Caracterização biofísico-química da enzima Orotato Fosforibosiltransferase (OPRT, EC 2.4.2.10) de Mycobacterium tuberculosis H37Rv : modelo para o desenvolvimento de novas drogas anti-tuberculose
... ADSL crystallographic data could not provide unequivocal evi- dence of amino acid residues involved in substrate binding specific- ity, especially for being a single substrate enz[r] ...
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Refinement of the Central Steps of Substrate Transport by the Aspartate Transporter GltPh: Elucidating the Role of the Na2 Sodium Binding Site.
... During substrate translocation, accessibility of the substrate binding site changed from the extracellular to the intracellular ...nm. Binding of substrate and sodium has been shown to ...
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In silico analysis of binding interaction of conantokins with NMDA receptors for potential therapeutic use in Alzheimer ’s disease
... Binding residue information for conantokin G only suggests Glu2, Gln6, Asn8, Arg13, Asn17 and Lys15 as predominant residues that are involved in interac- tions with the NMDAR subtype selectivity for NR2B subunit. ...
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MATERIALS AND METHODS Substrate and containers
... a substrate with sand and vermiculite (1:1 ...The substrate received the following nutrient solutions: Hoagland with 3 µM P (S1); Long Ashton II with ...v/v) substrate was irrigated with deionized ...
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J. Braz. Chem. Soc. vol.28 número3
... the binding mode of tiliroside to ...new binding site may contribute to the opening of new paths in the search for natural product inhibitors of this ...
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Dystrophin is a microtubule-associated protein
... An indirect link between dystrophin and MTs mediated by ankyrinB was recently shown to be important for proper trafficking of dystrophin and dystroglycan to the sarcolemma (Ayalon et al., 2008). However, costameric ...
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The zinc transporter, Slc39a7 (Zip7) is implicated in glycaemic control in skeletal muscle cells.
... Total soluble protein (100 m g) from the scramble control and siRNA-Zip7 transfected C2C12 cell lines was resolved on a 4–15% SDS-PAGE gradient gel (BIORAD) and transferred to a nitrocellulose membrane. The membranes ...
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Conservation of carbohydrate binding interfaces: evidence of human HBGA selection in norovirus evolution.
... strains binding to either A/B/H antigens (A/B binding groups, yellow) or Lewis antigens (Lewis binding group, ...conserved binding sites with other GII genotypes and thus may represent a ...
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MATERIAL AND METHODS Inoculum source and enrichment phase
... efficient cellulose conversion into sugars that can be easily fermented. The soluble carbohydrates consumed in C1, C2, and C3 were 58, 66, and 74%, respectively. There was higher consumption of soluble sugars in the ...
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