[PDF] Top 20 Biochemical studies of a membrane protein: The KtrAB ion transporter
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Biochemical studies of a membrane protein: The KtrAB ion transporter
... and protein was eluted in buffer B supplemented with either 5 mM ADP or ...ATP. Protein was then concentrated to ~5 mg/ml in a Sartorius Vivaspin column (15mL with Mw-cutoff of 30 kDa) and further ... See full document
77
Inhibition of dopamine transporter activity by G protein βγ subunits.
... through the Dopamine Transporter (DAT) is the primary mechanism of terminating dopamine signaling within the brain, thus playing an essential role in neuronal ...Deregulation of ... See full document
9
Protective Effects of Ferulic Acid on High Glucose-Induced Protein Glycation, Lipid Peroxidation, and Membrane Ion Pump Activity in Human Erythrocytes.
... effect of chronic hyperglycemia contributes to the development of pathogenesis associated with ...to protein glycation [18]. Normally, erythrocytes uptake glucose from the extracellular ... See full document
12
Ion-exchange membranes for protein crystallization and protein crystals’ derivatization
... pressure. The rate of ion transport through the membrane was estimated in order to know the concentration of heavy atom in the protein solution along ... See full document
190
Liposomes as a Model for the Biological Membrane: Studies on Daunorubicin Bilayer Interaction
... studied the interaction of the antitumoral drug daunorubicin with the membrane of unilamellar liposomes composed of ...on the overexpression of ... See full document
7
Biochemical and molecular characterization of barley plastidial ADP-glucose transporter (HvBT1).
... ADP-glucose transporter protein plays an important role in starch ...for the transport of ADP-glucose, the main precursor for starch biosynthesis during grain filling, from the ... See full document
9
The p7 protein of hepatitis C virus forms structurally plastic, minimalist ion channels.
... resolve the complete structure of native viroporins with an accuracy adequate to determine their ion-channel mechanisms face particular ...from the intrinsic difficulties in preparing large ... See full document
10
Molecular Biochemical Parasitology Characterization of phosphodiesterase-5 as a surface protein in the tegument of
... agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing ...and the development of an effective vaccine still remains the most desirable ... See full document
10
Biochemical and spectroscopic characterization of the membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617
... both the low-pH and the high-pH forms ...pH, the EPR results indicate the presence of a solvent-exchangeable proton which should be bound to a ligand of the molybdenum ... See full document
13
Dissecting the Molecular Mechanism of Nucleotide-Dependent Activation of the KtrAB K+ Transporter
... initiating the DTNB modification reaction, KtrB in DTNB buffer (20 mM Tris-HCl pH ...excess of KtrA C0 in buffer C without DTT while keeping the detergent concentration well above its critical ... See full document
21
The structure of the KtrAB potassium transporter
... determined the structure of a KtrAB potassium transporter with diffraction data to ...out of many thousands of crystals tested. The structure was determined from an ... See full document
15
Studies towards unveiling the association of Gla-rich protein with osteoarthritis
... is the strongest predictor of OA development with 64% of the affected population having more than 60 years old ...consequence of a degenerative process occurring in stable articular ... See full document
237
CHLORIDE ION PERMEABILITY STUDIES OF METAKAOLIN BASED HIGH PERFORMANCE CONCRETE
... cement of 53 grade manufactured by Birla Company confirming to IS 12269 was used in this ...investigation. The specific gravity of the cement was 3.06. The initial and final setting ... See full document
7
Structural and Functional Studies of H. seropedicae RecA Protein - Insights into the Polymerization of RecA Protein as Nucleoprotein Filament.
... HsRecA protein has a greater capacity to bind ssDNA in the presence of SSB, and this may reflect the plant-bacteria interaction and the apparent absence of some auxiliary ... See full document
25
Biochemical effects of protein glycation by methylglyoxal in Saccharomyces cerevisiae
... one of the most ubiquitous chemical reactions in food chemistry and biological ...systems. The expression Reaction du Maillard was introduced by the French chemist Louis Camille Maillard who ... See full document
244
The powerful high pressure tool for protein conformational studies
... Increasing protein concentration in- creases the likelihood of ...low protein concentration, a simple two-state, reversible unfolding tran- sition of recombinant PrP was ...observed. ... See full document
9
Analysis of surface protein expression reveals the growth pattern of the gram-negative outer membrane.
... elucidate the role of growth in OM organization, we examined the spatial pattern of initial secretion and subsequent redistribution of the abundant OM protein LamB ... See full document
10
Study of the self-assembly of the pro-inflammatory S100A9 protein driven by metal ion binding
... with the resuspension of the cells in 20 mM Tris-HCl pH 8, with DNase (for DNA degradation) and ...mM of PMSF (to inhibit ...min. The supernatant was discarded and the pellet ... See full document
70
Analysis of membrane protein genes in a Brazilian isolate of Anaplasma marginale
... sequencing of the complete genome of Anaplasma marginale has enabled the identification of several genes that encode membrane proteins, thereby increasing the chances ... See full document
7
Position of the third Na+ site in the aspartate transporter GltPh and the human glutamate transporter, EAAT1.
... Pure protein was reconstituted into liposomes using a method modified from Gaillard et ...ratio of 3:1, were mixed, dried under nitrogen, and resuspended in internal buffer (100 mM KCl, 20 mM HEPES pH ...or ... See full document
10
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