[PDF] Top 20 Different methods of real-time PCR for detection of pseudorabies virus
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Different methods of real-time PCR for detection of pseudorabies virus
... ABSTRACT: Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine ...aim of this study was to evaluate different genomic regions, ... See full document
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Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
... phylogeny of currently circu- lating lineages of each DENV ...probe of the new CDC DENV-1–4 RT-PCR was compared to the corresponding serotype alignment and determined the frequen- cy of ... See full document
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Detection and typing of highly pathogenic porcine reproductive and respiratory syndrome virus by multiplex real-time rt-PCR.
... strain of type 2-PRRSV (HP), causing high fever and severe morbidity and mortality in pigs of all ages, emerged in swine farms all over China ...deletion of 30 amino acids (aa) in the non-structural ... See full document
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Development of a real-time PCR assay for the detection of the golden mussel (Limnoperna fortunei, Mytilidae) in environmental samples
... stages of its colonization. To this end, Pie et al. (2006) developed a set of species-specific primers that could be used to detect golden mussel larvae obtained from filtered environmental ...approach ... See full document
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REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... level of detection was ...value of 36.6 ± 1.6. The real-time PCR assay we developed was approximately 1000-fold more sensitive than conventional PCR assays (gel ... See full document
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Real-Time PCR for Measles Virus Detection on Clinical Specimens with Negative IgM Result in Morocco.
... confirmation of measles cases represents an important indicator regarding the performance of the measles-elimination program, the aim of this study was to evaluate the effectiveness of the ... See full document
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Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
... amount of a targeted nucleic acid expressed as a copy number or ...knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable ...objective of this ... See full document
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Comparison of Four Extraction Methods to Detect Hepatitis A Virus RNA in Serum and Stool Samples
... efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as ... See full document
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Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
... 100mL of Bolton broth with a selective supplement (SR183E, Oxoid, Basingstoke, UK) and was subjected to a washing process by rinse friction for approximately 1min (bioMérieux, ...aliquots of the Bolton ... See full document
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Detection of infectious laryngotracheitis virus by real-time PCR in naturally and experimentally infected chickens.
... The real-time PCR assay was also found to be highly reproducible throughout the study and was able to determine the level of viral DNA in different tissues of the ILTV infected ... See full document
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DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR ASSAYS FOR THE DETECTION OF NOSOCOMIAL PATHOGENS Deepa Anbazhagan
... source of morbidity and mortality. It is necessary to develop rapid detection assays of nosocomial pathogens for better prognosis and initiation of antimicrobial therapy in ...development ... See full document
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Preclinical detection of porcine circovirus type 2 infection using an ultrasensitive nanoparticle DNA probe-based PCR assay.
... efficiency of functionalized Au-NPs with different oligonucleotides in UNDP-PCR ...quantification of barcode DNA by real- time PCR when compared with the Au-NPs coated ... See full document
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Accurate detection and quantification of the fish viral hemorrhagic Septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay.
... Septicemia virus (VHSv) is one of the world’s most serious fish pathogens, infecting ...series of outbreaks in subsequent years, and shut down interstate transport of ...of ... See full document
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Detection of bacterial 16S rRNA and identification of four clinically important bacteria by real-time PCR.
... two different instruments, the Roche Light Cycler 480 II (LC 480 II) with SYBR Green I Master Mix (Roche Applied Sciences, Indianapolis, IN), and the BioRad CFX96 with SsoAdvanced SYBR Green supermix (Bio-Rad ... See full document
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Rev. Soc. Bras. Med. Trop. vol.50 número3
... alternative methods for the accurate detection of infectious and parasitic diseases, such as the ...evaluation of a duplex real- time quantitative PCR (qPCR) protocol ... See full document
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Helena Lage FerreiraI Fernando Rosado Spilki
... volume of 25µL containing 1µL of cDNA ...standards of purified PCR product for each ...fluorescence detection at the end of the annealing-extension ...fluorescence ... See full document
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Arq. Gastroenterol. vol.52 número2
... status of these DQA and DQB alleles in potential CD patients or risk populations (celiac disease patient’s irst degree relatives, people with related autoimmune diseases, among others) could dramatically aide in ... See full document
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Canine distemper virus detection by different methods of One-Step RT-qPCR
... Benefits of SYBR Green I One- Step RT-qPCR assays over conventional RT-PCR and two-step qPCR detection methods, particularly for diagnosis, include their large dynamic range, ability to be ... See full document
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Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
... sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods (NAKATANI et ...Isolation of bacterial DNA in tissues is a highly complex procedure, ... See full document
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Detection of genetically modified maize events in Brazilian maize-derived food products
... threshold of 1% for food labeling, which underscores need for monitoring ...and different types of nacho chips were analyzed by conventional and real-time PCR in 2011 and ...most ... See full document
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