[PDF] Top 20 Directed evolution of lichenicidin

... maturation of the Bliβ peptide implies two proteolytic cleavages: i) the removal of leader peptide by LicT and ii) the removal of six N-terminally located amino acids, commonly designated by ... See full document

... role of mutations on the enzyme variant properties, including the lower production yields and the photo inactivation of the FAD cofactor, responsible for the absence of activity in purified 2C9, ... See full document

... addition of FN3-alkaline phosphatase fusion protein of TA1, TA7 and TA8 that had been diluted to 5 μg/ mL in PBS containing ...specificities of three TA8 variants, 2H7, 2H10 and 3C12, phage ELISA was ... See full document

... mutation of Ile30 to Thr, helix 3 is repositioned, evident in the movement of residues 82 and 85 with respect to residue 30 and the loss of the interaction between Leu61 and Phe71 in 10- CM (Figure ... See full document

... downstream of the target protein bridged by a TEV protease ...production of TEV protease to allow for in situ cleavage of the target protein-sfGFP fusion to prevent any potential adverse downstream ... See full document

... activities of lantibiotics, especially their antibacterial activity, has been of much interest (Cortes et ...class of ribosomally synthesized peptides that undergo extensive posttranslational ... See full document

... set of experiments designed and each question that the ex- periments aim to answer, together with the basic configuration of EDGE that was ...parameters of EDGE itself, with two of them, the ... See full document

... Influence of the feedback regulation in total is classified into negative FBL (see examples marked by D in ...composed of genes #10 and #13 in ...composed of the nodes and their connections are ... See full document

... members of the Saccharinae subtribe, we identified the fast evolving ...region of the sugarcane genome in the Sugarcane Expressed Sequence Tag (SUCEST) genome project ...group of Saccharum ...some ... See full document

... example of spite (Gardner & West, 2004a; Gardner et al, 2004; West et al, ...members of the same or close species (for reviews on bacteriocins see (Baba & Schneewind, 1998) and on colicins see ... See full document

... structure of each subunit of the homotetramer has a peanut- shaped with three distinct ...association of the protein monomers, an head domain with a putative function associated with immobilization ... See full document

... deamidation of glutamine and asparagine residues triggered by high temperatures that contributes to inactivation of irreversibly denaturated proteins ...formation of hydrogen bonds and/or salt ... See full document

... spreadsheet evolution were already proposed [Cunha et ...this evolution had to be done manually, and a human operator had to apply successive transformations to the spreadsheet model until it reached the ... See full document

... induction of three reactions of DNA somatic modification employed by jawed vertebrates in the context of adaptive immunity: Somatic Hypermutation (SHM), Class Switch Recombination (CSR) and ... See full document

... extent of NF-kB activation and TNF-a expression were significantly ...surfaces of monocytes and macrophages to amplify the inflammatory effects of ...release of inflammatory ... See full document

... study of complex networks has become a hot topic in various fields like physics, mathematics, biology, social sciences, computer sciences, and so on ...Most of complex networks have two properties: ... See full document

... overview of the development of different finаnciаl institutions meet the criteriа of Islаm аnd their evolution since the 70s in the privаte sector аcross the Muslim ...restructuring of ... See full document

... set of functions is defined, where each function from that set invokes a model operation and a data operation, receiving a model/data pair and other arguments ...arguments of the ...position of a ... See full document

... α­peptides of two­component lantibiotics resemble mersacidin and they also possess the conserved motif that is presumably involved in binding lipid ...binding of an antibiotic to a complex biosynthetic ... See full document

... tity of ef fec tive dose equiv a lent and the new qual ity fac tor, the dose con tri bu tion of ra don and thoron be came more ap par - ent as you can see in ...Half of this dose came from ra don and ... See full document