[PDF] Top 20 MATERIALS AND METHODS Microorganism and media components

... individual and interactive effects of these nutrient variables were studied by conducting the fermentation runs at randomly selected different levels of all four ...data and predicted by quadratic model are ... See full document

... psychrophiles and psychrotrophs inhabit both terrestrial and aquatic environments in polar and alpine regions, in the bulk of the ocean, in shallow subterranean regions, in the upper atmosphere, in ... See full document

... from Bacillus sp SMIA-2 retained more than 70% activity after incubated for 1 h at 50ºC with sodium dodecyl sulphate, an anionic detergent (Fig. 7). However, very little residual activity was obtained with sodium ... See full document

... characteristics and enzyme production were ...husk and bird performance characteristics when employed as poultry diet additive were ...6.0 and 55 o ...Tween-80 and SDS yielded endoglucanase ... See full document

... porosity and subsequent cell lysis due to extraction of membrane ...supplement and subsequent utilization of precursor component (linoleic acid) for GLA ...oil) and culture grown in supplemented ... See full document

... Isomaltulose [O-α-D-glucopiranosyl-(1→6)-β-D-frutofuranose] is a reducing disaccharide commercially obtained by the enzymatic conversion from sucrose catalyzed by a microbial glucosyltransferase as shown in Fig. 1. ... See full document

... substrate and growth ...carbonate and 1.3% of calcium sulphate (dry mass basis) and then packed (250 g) in polypropylene (PP) bags (18x30 ...temperature and inoculated with three 15 mm ... See full document

... DEAE-Cellulose and CM-Cellulose chromatography, and purified 141-fold, with ...Cellulose and Concanavalin A-Sepharose chromatography, with ...Temperature and pH optima were in the range of ... See full document

... nature and the alkalophilic Bacillus strains are often good sources of alkaline extracellular enzymes (11, ...actinomycetes and bacteria (13, ...fat and protein debris by chemical processes is both ... See full document

... No inhibitory effect was observed at 1, 2, 5 and 10 mM of EDTA. However, with 10 mM the enzyme retained about 84% of its activity when incubated for 30 min. This finding was similar to that of Tunga et al. (19), ... See full document

... At higher concentrations, saturation is evidenced. It was also noticed that at the two highest cell densities, ISM- images exhibited less contrast. Images with lower contrast lead to less detection probability for cells ... See full document

... intermediate. This makes laccase the best candidate for the environmentally benign process (12). Laccases are currently seen as highly interesting industrial enzymes because of their wide variety of potential substrates. ... See full document

... organized and complex cell envelope, formed by a 30 to 54 carbon atom-containing peptidoglycan-arabinoglycan skeleton bonded to mycolic acids that represent more than 40% of the total cell ...dimycolates ... See full document

... 1 and Figure 3, the major saturated fatty acid found at all cultivation temperatures was palmitic acid (C16:0) and the major polyunsaturated fatty acid was DHA ...C16:1 and C18:1 were also found but ... See full document

... (EPS) and mycelial ...phosphate and C/N ratio was determined by single factor ...medium components were investigated using the orthogonal matrix ...O, and for mycelial growth was 50 g/l ... See full document

... of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the medium were measured during cultivation. From five cultivations carried out under the same conditions, a mean ... See full document

... (Salomon and Silva Lora, ...years and consequently the vinasse ...Livestock and Supply reported that, for the year 2030, ethanol production could reach 67 million cubic ...fields and this ... See full document

... thermostable and was able to withstand the thermal ...unique and did not correspond with the properties of the species earlier ...2+ and Zn 2+ ... See full document

... branched and unbranched free ...perimeter, and convex perimeter) but only two parameters (perimeter and convex perimeter) were chosen to automatically classify the cells into the four morphological ... See full document

... cultivation media which use dialyzed yeast extract (Table 1), concerns the fact that it is not necessary to dialyze any of its components, resulting in an additional operational ...practice) and the ... See full document