Top PDF A molecular assay for sensitive detection of pathogen-specific T-cells.
A molecular assay for sensitive detection of pathogen-specific T-cells.
... variety of pathogens, including CMV and MTB ...sensitivity of the standard IFN-c Elispot ...antigen- specific immune responses of very small populations of cells and is gaining ...
10
Rapid and sensitive detection of an intracellular pathogen in human peripheral leukocytes with hybridizing magnetic relaxation nanosensors.
... aim of this study was to develop and test a nanoparticle-based assay for the sensitive detection of genomic bacterial biomarkers of an intracellular pathogen in clinical ...
13
Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi
... methods of isolation and detection of Phytophthora in oak roots, based mainly on baiting methods, however, are of relatively low effectiveness and ...More sensitive, molecular ...
9
Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay
... limit of detection for target DNA was 1 pg (Figure 3) and no amplification was obtained up to a dilution of ...fragment of a target DNA give high sensitivity and the detection limit is ...
5
Multiplex Real-Time qPCR Assay for Simultaneous and Sensitive Detection of Phytoplasmas in Sesame Plants and Insect Vectors.
... Diagnosis of phytoplasma pathogens has proven difficult because they cannot be cul- tured in vitro ...advent of molecular techniques, disease symptoms, insect or dodder graft transmission to host ...
14
Modulation of CD4(+) T Cell-Dependent Specific Cytotoxic CD8(+) T Cells Differentiation and Proliferation by the Timing of Increase in the Pathogen Load
... 5. Specific cytotoxicity in C57BL/6 mice challenged with irradiated or non-irradiated trypomastigotes of ...Groups of C57BL/6 mice were challenged or not ...trypomastigotes of the Y strain ...
9
Establishment and validation of whole-cell based fluorescence assays to identify anti-mycobacterial compounds using the Acanthamoeba castellanii-Mycobacterium marinum host-pathogen system.
... cousin of M. tuberculosis, M. marinum is sensitive to most standard antibiotics used to treat tuberculosis, and also used to validate various screenings protocols ...infection assay with an MIC ...
13
Sensitive and Specific Biomimetic Lipid Coated Microfluidics to Isolate Viable Circulating Tumor Cells and Microemboli for Cancer Detection.
... tumor cells (CTCs) and circulating tumor microemboli (CTM) directly from whole blood of all stage clinical cancer ...clustering of lipid-tethered antibodies to CTC antigens and minimized ...
21
Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.
... for detection of residual undifferentiated hiPSCs in hiPSCs-derived differenti- ated cells: soft agar colony formation assay, flow cytometry and ...disadvantages of the assays ...
9
Sensitive detection of pre-existing BCR-ABL kinase domain mutations in CD34+ cells of newly diagnosed chronic-phase chronic myeloid leukemia patients is associated with imatinib resistance: implications in the post-imatinib era.
... This assay can detect 18 of the most clinically relevant and common BCR-ABL mutations ...any of the reagents, PCR mix formulations and thermal profile ...sequences of ASO primers ...
9
Identification of bovine material in porcine spray-dried blood derivatives using the Polymerase Chain Reaction technique
... theory of bovine spongiform encephalopathy (BSE) spread in cattle by contaminated animal feeds, screening of feed products has become ...ingredients of foods for both pets and farm ...use of ...
7
A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis.
... number of amplicons in the partial ...more of the eight remaining loci; 15 of these Figure ...on molecular weight and fluorescent dye ...red-labeled specific amplicons, all of ...
8
Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
... (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus ...set of LAMP primers was designed based on the sequence of the ASFV ...
7
Clinics vol.68 número8
... detectable using the MHT, for which the sensitivity and specificity of the screening is known to be very good (1,2). However, the weakest aspect of the MHT is specificity. In a recent study using K. ...
1
THE ROLE OF NANOTECHNOLOGY IN CANCER TREATMENT AND DIAGNOSIS
... application of different polymer-based drug delivery systems ...kinds of polyme- Several kinds of polyme- ric based-NPS ...treatment of various cancers (Miele et ...characteristic of ...
9
Ultra-fast analysis of plasma and intracellular levels of HIV protease inhibitors in children: a clinical application of MALDI mass spectrometry.
... measurements of the ...speed of 1 mm per second while firing of the laser at 1,000 Hz, which corresponds to an analysis time of ...For specific setting see Table ...validity of ...
9
Radiol Bras vol.43 número3
... combination of positron emission tomography (PET) with magnetic resonance imaging (MRI) has been the subject of several studies in recent ...most sensitive and specific imaging modality in the ...
7
Functional characterization of aquaporin-4 specific T cells: towards a model for neuromyelitis optica.
... transfer of immunoglobulin fractions from NMO patients did not induce astrocytic damage in laboratory animals whose blood brain barrier was leaky in the absence of inflammatory stimuli [22] suggesting that ...
11
Rev. Soc. Bras. Med. Trop. vol.44 número5
... Samples were inoculated onto Enterococcosel ™ agar (BBL, USA) supplemented with 6mg of vancomycin per mL and incubated at 35ºC for up to 72h. Suspicious colonies (Esculin positive) were planted onto sheep blood ...
2
Direct ex-vivo evaluation of pneumococcal specific T-cells in healthy adults.
... incidence of pneumococcal infections. However with time, the rate of colonisation and infection falls and by late childhood the prevalence of nasal colonisation reaches a low-point – a state that ...
7
