[PDF] Top 20 Quantitative analysis of the dystrophin gene by real-time PCR
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Quantitative analysis of the dystrophin gene by real-time PCR
... makes the determination of carrier status very important for genetic counseling and prevention of the ...Multiplex PCR technique has been widely used for the detection of ... See full document
6
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
... clone of Chytridiomycota (HM104573) belonging to groups III and IV, respectively), clustered with their corresponding reference ...rooted the analysis together with 3 clones corresponding to fungal ... See full document
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... Recently, the availability of the full sequence of the melon genome and multiple transcriptome data has inspired many diverse molecular biology studies performed in melons [2,19,31– ... See full document
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A QUANTITATIVE ANALYSIS OF PROPIONIBACTERIUM ACNES IN LESIONAL AND NON-LESIONAL SKIN OF PATIENTS WITH PROGRESSIVE MACULAR HYPOMELANOSIS BY REAL-TIME POLYMERASE CHAIN REACTION Silvana Maria de Morais Cavalcanti
... about the etiology of progressive macular hypomelanosis, although it has been suggested that Propionibacterium acnes plays an important ...determine the cut-off point for the number of ... See full document
7
Arq. NeuroPsiquiatr. vol.56 número4
... limitation of neck and trunk flexure, compatible with rigid spine syndrome. The serum muscle enzymes were somewhat elevated and the electromyography showed a myopatic ...change. The muscle ... See full document
7
Arq. NeuroPsiquiatr. vol.68 número4
... investigate the pattern of expression of protein (immunohistochemistry) and gene (real-time PCR) Hsp70 in experimental focal cerebral ischemia in rats by occlusion ... See full document
5
Fast detection of deletion breakpoints using quantitative PCR
... samples by using a Puregene DNA extraction kit (Gentra Systems) ac- cording to the manufacturer’s ...synthesized by Integrated DNA technologies (Coralville, IA, USA). The primers used are ... See full document
5
Host immune response to mosquito-transmitted chikungunya virus differs from that elicited by needle inoculated virus.
... mice of the same age. In both of these experimental groups, six mice were used for each time ...Three of the mice in each group were used for cytokine expression analysis ... See full document
8
Arq. Gastroenterol. vol.52 número2
... status of these DQA and DQB alleles in potential CD patients or risk populations (celiac disease patient’s irst degree relatives, people with related autoimmune diseases, among others) could dramatically aide in ... See full document
4
The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)
... All PCR primers for real-time quantitative RT-PCR are shown in Table ...products, of around 200 bp of β-actin and OIH. The β-actin (EF667345) and OIH (NM_001030612) ... See full document
6
Five years MIQE guidelines: the case of the Arabian countries.
... The quantitative real time polymerase chain reaction (qPCR) has become a key molecular enabling technology with an immense range of research, clinical, forensic as well as diagnostic ... See full document
6
Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
... described the multiplex assays used to analyse ccf nDNA and ccf mtDNA ...Using the optimised PCR protocol, the amplification of nDNA and mtDNA in a single reaction tube was very simi- ... See full document
5
High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.
... amplified the hsp70 234 bp fragments for all species analyzed in this study using the primers described by Graça et ...[17]. The alignment of the nucleotide sequence of ... See full document
18
Bruno Antônio Marinho Sanchez3
... for the diagnosis of malaria infection are expected to accurately identify submicroscopic parasite ...number of PCR protocols have been described, few studies have addressed the ... See full document
8
Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model.
... Japan). The cDNA was amplified in triplicate using real-time PCR with the ABI Prism 7700 Sequence Detection System (Applied Biosystems, Lincoln, CA) using optical grade 96- well ... See full document
11
Rev. Soc. Bras. Med. Trop. vol.50 número3
... for the accurate detection of infectious and parasitic diseases, such as the ...leishmaniases. The present study describes the optimization and evaluation of a duplex ... See full document
8
A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.
... that the increased AMP expression observed upon Pvr loss proceeds through Rel, we then examined the expression of att in S2 cells that were simultaneously treated with Pvr and Rel ...depletion ... See full document
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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive technique for gene expression analysis, depends on the stability of the ... See full document
10
Location-specific epigenetic regulation of the metallothionein 3 gene in esophageal adenocarcinomas.
... MT3. Analysis of three different regions of the MT3 promoter demonstrated significantly high levels of H3K4me2 and H3K9ace, and low levels of H3K9me2 in all three regions in ... See full document
10
Critical role of the virus-encoded microRNA-155 ortholog in the induction of Marek's disease lymphomas.
... generation of viruses with mutations in all of the miRNAs within the cluster, we synthesised a gene designed to introduce mutations into the stem loop structures of each ... See full document
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