[PDF] Top 20 Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay
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Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay
... a real-time PCR method based on the use of speci fic TaqMan probes that proved to be a powerful tool, highly speci fic, sensitive and accurate for soybean detection and ... See full document
6
Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
... Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine ...the detection and quantification of seed-borne inoculum such as the ... See full document
6
Quantitative analysis of the dystrophin gene by real-time PCR
... determination of carrier status very important for genetic counseling and prevention of the ...Multiplex PCR technique has been widely used for the detection of common deletions ... See full document
6
Development of Real Time PCR Meat detection kits and technology market transfer
... inserted in the minor groove of the dsDNA, at the end of each PCR cycle (Navarro et ...binding of these dyes increases its fluorescence, allowing the detection by the ... See full document
132
Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
... performance of the modified lipl32 TaqMan real-time PCR assay was compared with results obtained from a well-established conventional PCR in which a Leptospira ... See full document
7
Detection of A. phagocytophilum and E. chaffeensis in patient and mouse blood and ticks by a duplex real-time PCR assay.
... fragments of the ...The PCR products were purified with a Gel Extraction and PCR Purification Combo Kit (Spin-column, BioTeke Corporation, Beijing), ligated into the pEASY-T1 vector and then ... See full document
7
Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses.
... fatal in spite of modern intensive care, especially some HFVs can be transmitted from human to human and consequently threat to cause epidemics with high mortality rates ...phase of VHFs, a rapid and ... See full document
11
Development of a real-time PCR assay for the detection of the golden mussel (Limnoperna fortunei, Mytilidae) in environmental samples
... sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene of the golden mussel available on genbank, particularly those sequenced by Ghabooli et ...amplification of a short ... See full document
5
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
... group in soil biological diversity and ...knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative ... See full document
13
The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas
... introduction of real-time ...progress of the PCR at every cycle as it occurs, using ...Today, real-time PCR is the method of choice for studying changes ... See full document
6
Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
... effective in removing heat and toxic substances. Most of traditional Chinese medicines are been distributed in desert regions, which leads to their ...shortage. In recent years, there has been ... See full document
9
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... run in a 7300 RT-qPCR thermocycler (Applied Biosystems, Foster, CA, USA) using the following cycling parameters: 50°C for 2 min, 95°C for 10 min, 45 cycles at 95°C for 2 min, 62°C for 30 seconds and 72°C for 30 ... See full document
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REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... level of detection was ...amplification in all replicates) with the CT value of 36.6 ± 1.6. The real-time PCR assay we developed was approximately 1000-fold more ... See full document
9
One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil
... goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and ...quantification of RNA hepatitis C virus virus load in ... See full document
6
Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... caused by the bacterium “Candi- datus Liberibacter ...non-cultivability of the bacterium has severely hampered studies on the ...assignment of close relationships at the genus and species levels. ... See full document
8
Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus.
... The Assay includes a set of oligonucleotide primers and dual- labeled 59-fluorescent (Taqman) probes for in vitro, qualitative detection of ...regions of DENV RNA are ... See full document
15
Quantitative detection of Borrelia burgdorferi sensu lato in erythema migrans skin lesions using internally controlled duplex real time PCR.
... quantified in human skin biopsies [19], however to our knowledge, quantification of ...garinii in European human skin biopsies has not yet been ...duplex quantitative real time ... See full document
9
Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes
... The real-time FRET PCR protocol provides an al- ternative to the classic or more modern molecular or serological methods for the detection of ...japonicum in snails, the first ... See full document
6
Quantitative real-time PCR for the clinical detection of Helicobacter pylori
... diagnosis of Helicobacter pylori infection is very important in both clinical practice and ...sensitivity of real-time PCR (RT-PCR) for the detection and ... See full document
4
Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay
... assessment in this ...diluted in different proportions in nuclease free water and tested for PCR ...limit of detection for target DNA was 1 pg (Figure 3) and no amplification was ... See full document
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