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[PDF] Top 20 Reprogramming of Human Somatic Cells Using Human and Animal Oocytes

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Reprogramming of Human Somatic Cells Using Human and Animal Oocytes

Reprogramming of Human Somatic Cells Using Human and Animal Oocytes

... enucleation of the denuded MII oocytes was performed using a small piezo-driven ...staining and UV light exposure. The enucle- ated oocytes were injected with nuclei from fresh ... See full document

12

Induction of pluripotent stem cells by reprogramming human ocular fibroblasts under xeno-free conditions

Induction of pluripotent stem cells by reprogramming human ocular fibroblasts under xeno-free conditions

... penicillin and streptomycin in aseptic PBS solution, and cut into 1 × 1 mm tissue blocks using ophthalmic micro-scissors in a 60-mm tissue culture ...the cells were incubated with Dulbecco’s ... See full document

8

MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells.

MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells.

... initiation and progression. Previous studies have shown that reprogramming of colon cancer cells using Oct3/4, Sox2, Klf4, and cMyc reduces cancer ...cancer cells. It was ... See full document

23

Plasticity of adult human pancreatic duct cells by neurogenin3-mediated reprogramming.

Plasticity of adult human pancreatic duct cells by neurogenin3-mediated reprogramming.

... Quality of TRIzol (Gibco BRL, Carlsbad, CA, USA) extracted RNA was verified on a 2100 Bioanalyzer (Agilent, Waldbronn, Germany), taking minimal cutoff RIN $ ...pool of 3 independent donor pancreata. The ... See full document

11

Arq. NeuroPsiquiatr.  vol.69 número1

Arq. NeuroPsiquiatr. vol.69 número1

... Several animal experimental models have been used in the study of malignant ...objective of the study was to test the efficacy of a simple, reproducible and low cost animal ... See full document

6

Reprogramming of round spermatids by the germinal vesicle cytoplasm in mice.

Reprogramming of round spermatids by the germinal vesicle cytoplasm in mice.

... extracts of Xenopus oocytes can remodel mammalian somatic cell genomes [32-36], although GV and MII stage oocyte cytoplasm from amphibians and mammals have shown different ... See full document

7

Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.

Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.

... iPSCs Reprogramming and Characterization Induced pluripotent stem cell lines were derived from primary skin cells obtained from skin ...University of Alabama at Birmingham Institutional Review ... See full document

10

Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.

Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.

... accuracy of replicating the genetic code is ...fidelity of the genome ensuring a low error rate between ...similarity of individuals whilst providing a repertoire of variants for ...the ... See full document

15

Exploring cell reprogramming techniques for Angelman Syndrome disease modelling

Exploring cell reprogramming techniques for Angelman Syndrome disease modelling

... Lineage reprogramming technique represents a time-saving process, when compared to other reprogramming approaches, since iNs are obtained within two to three weeks upon transcription factor overexpression, ... See full document

63

In vitro epigenetic reprogramming of human cardiac mesenchymal stromal cells into functionally competent cardiovascular precursors.

In vitro epigenetic reprogramming of human cardiac mesenchymal stromal cells into functionally competent cardiovascular precursors.

... Adult human cardiac mesenchymal-like stromal cells (CStC) represent a relatively accessible cell type useful for ...aim of the present work was to reprogram CStC into functionally competent ... See full document

12

Reprogramming of embryonic human fibroblasts into fetal hematopoietic progenitors by fusion with human fetal liver CD34+ cells.

Reprogramming of embryonic human fibroblasts into fetal hematopoietic progenitors by fusion with human fetal liver CD34+ cells.

... with somatic cell nuclear transfer, inter-cellular hybrid formation_ENREF_3, and ectopic expression of transcription factors have clearly demonstrated that cell fate can be dramatically altered by ... See full document

9

The mechanism of gene targeting in human somatic cells.

The mechanism of gene targeting in human somatic cells.

... locations of crossovers are determined by the initial positions of HJ formation and branch migration ...flies and mouse embryonic stem cells, which revealed an exponential retention ... See full document

14

Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

Release of sICAM-1 in oocytes and in vitro fertilized human embryos.

... Cytokines and chemokines presence were measured in embryo culture supernatants by Bio-Plex cytokine assay (Bio-Rad Labo- ratories, Hercules, CA) [15,16] described by the ...measurement of multiple cytokines ... See full document

7

Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain.

Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain.

... 9.0), and the dissolved solution was applied to CM sepharose Fast Flow column, washed with the same buffer to remove unattached peptides and ...peptides and proteins were eluted with linear gradient ... See full document

8

Figueiredo a Leal Santos Baptista de

Figueiredo a Leal Santos Baptista de

... In vitro neural differentiation of human embryonic stem cells using a. low-density mouse embryonic fibroblast feeder protocol[r] ... See full document

75

Divergent JAM-C Expression Accelerates Monocyte-Derived Cell Exit from Atherosclerotic Plaques.

Divergent JAM-C Expression Accelerates Monocyte-Derived Cell Exit from Atherosclerotic Plaques.

... mice, and these were sacrificed 4-days later (Fig 1A and ...Immunostaining of serial sections for CD68 + was used to determine cells that were presumably monocytes and inflammatory ... See full document

27

Stromal cells in the hematopoietic-stem-cell niche

Stromal cells in the hematopoietic-stem-cell niche

... stem cells (MSC) are multipotential nonhematopoietic progenitor cells capable of differentiating into multiple mesenchymal ...functional human hematopoietic microenvironment and promote ... See full document

7

The membrane-associated transient receptor potential vanilloid channel is the central heat shock receptor controlling the cellular heat shock response in epithelial cells.

The membrane-associated transient receptor potential vanilloid channel is the central heat shock receptor controlling the cellular heat shock response in epithelial cells.

... sensor of pain and temperature variations that initiate the propagation of a signal among neuronal cells, but it may also act on neuronal as well as non-neuronal cells as a molecular ... See full document

12

Evaluation of Antimalarial Activity and Toxicity of a New Primaquine Prodrug

Evaluation of Antimalarial Activity and Toxicity of a New Primaquine Prodrug

... Chung and collaborators described the synthesis of the dipeptide phenylalanine-alanine-primaquine (Phe-Ala-PQ) as a prodrug of ...activity of Phe-Ala-PQ, this dipeptide has not been evaluated ... See full document

10

Challenging metastatic breast cancer with the natural defensin PvD1

Challenging metastatic breast cancer with the natural defensin PvD1

... MDA-MB-231 and MCF 10A were purchased from American Type Culture Collection (ATCC, HTB-26 and CRL-110317 ...endothelial cells) isolated by ScienCell Research Laboratories from human brain ... See full document

13

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