[PDF] Top 20 Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Has 5088 "Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)" found on our website. Below are the top 20 most common "Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)".
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
... Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNA was extracted using the UNIQ-10 column TRIzol kit (Shanghai Sangon Biotech, Shanghai, China) and ... See full document
9
Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
... of reverse transcriptase polymerase chain reaction (RT- PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird ... See full document
10
Development of a quantitative competitive reverse transcriptase polymerase chain reaction for the quantification of growth hormone gene expression in pigs
... expression, quantitative PCR is the simplest analytical laboratory ...competitive reaction, facilitating the study of gene expression patterns for both genetics and diagnostics of infectious ... See full document
6
A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... From June-August 2013, cases of vesicular stomatitis disease affecting horses and cattle were reported to the Veterinary Hospital of the Federal University of Campina Grande, Paraiba state, Brazil. These outbreaks ... See full document
6
Laboratory capacity building in Asia for infectious disease research: experiences from the South East Asia Infectious Disease Clinical Research Network (SEAICRN).
... Abbreviations: GCLP, good clinical laboratory practice; MDL, molecular diagnostic laboratory; pH1N1, influenza A (H1N1); RT-PCR, reverse transcriptase polymerase chain reaction; SEAICRN,[r] ... See full document
5
Arq. NeuroPsiquiatr. vol.74 número10
... (EV) reverse transcription polymerase chain reaction (RT-PCR) in CSF; this is the first study that tries to establish an acceptable maximum number of RBCs in CSF that does not influence ... See full document
6
Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction
... The reverse transcriptase-polymerase chain re- action (RT-PCR) protocol developed by Lanciotti et al. (1992) could detect and identify all four serotypes of dengue viruses simultaneously in a nested ... See full document
5
Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development
... Display Reverse Transcriptase – Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, ... See full document
7
Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
... of reverse transcription-polymerase chain reaction (RT-PCR) and sequence analyses, RVA strains are clas- sified into the G and P genotypes, which represent the two type-specific outer ... See full document
7
Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn’s disease
... The TB polymerase chain reaction (PCR) assay is an interesting and promising approach. Amarapurkar et al. (15) reported that using TB PCR for biopsy specimens was a valuable method to differentiate ... See full document
5
Dengue Virus Detection by Using Reverse Transcription- Polymerase Chain Reaction in Saliva and Progeny of Experimentally Infected Aedes albopictus from Brazil
... and reverse transcriptase-PCR am- plification (RT-PCR) - Viral RNA was extracted from the supernatant of infected cells by using the QIAmp Viral Mini Kit (Qiagen, ... See full document
6
Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil
... a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA ... See full document
6
Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
... After hair pulling, germinative buds of the initial anagen phase of new hair growth express tyrosinase mRNA, which can be detected by means of the reverse transcrip- tase polymerase chain ... See full document
5
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... Among the approaches available for gene expression studies, the quantitative polymerase chain reaction (qPCR) is extensively used. The qPCR is a fast, easy for analyzing and understanding gene ... See full document
7
Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection.
... [r] ... See full document
18
Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model.
... real-time quantitative polymerase chain reaction (PCR) were evaluated in human retinal pigment epithelial (ARPE-19) cells (A, C, E) and in human umbilical vein endothelial cells (HUVECs) (B, ... See full document
11
Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
... the reaction; for the first step, higher primer concentrations provided the best ...multiplex reaction, this effect was less pronounced because of the lower degeneration of the ...PCR reaction and ... See full document
9
Abnormal apoptosis of trophoblastic cells is related to the up-regulation of CYP11A gene in placenta of preeclampsia patients.
... placenta; reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine CYP11A expression at mRNA and protein levels in patients with severe ... See full document
8
Analysis of Respiratory Syncytial Virus in Clinical Samples by Reverse Transcriptase-Polymerase Chain Reaction Restriction Mapping
... The aim of this study was to develop a polymerase chain reaction (PCR) for the detection of respi- ratory syncytial virus (RSV) genomes. The primers were designed from published sequences and se- ... See full document
6
J. Appl. Oral Sci. vol.12 número1
... “Reverse transcription and polymerase chain reaction: principles and applications in dentistry”, which offers an interesting and exciting analysis of the potential that molecular ... See full document
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