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[PDF] Top 20 Self-contained induction of neurons from human embryonic stem cells.

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Self-contained induction of neurons from human embryonic stem cells.

Self-contained induction of neurons from human embryonic stem cells.

... Institute of Health (NIH) ...control of a CAG promoter (a gift from ...micrograms of the linearized plasmid was then electroporated into a suspension of hES cells (10 7 ... See full document

8

Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.

Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.

... originating from fibroblasts or from radial glia-like neural stem (NS) cells derived from human fetal cortex ...capacity of the different pluripotent stem cell ... See full document

13

Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

... GSC-like cells from human ESCs ...3% of the GSC-like cells could be differentiated into haploid male germ cells, exhibiting specific markers after only two weeks of ... See full document

13

Innate immunity in human embryonic stem cells: comparison with adult human endothelial cells.

Innate immunity in human embryonic stem cells: comparison with adult human endothelial cells.

... H7 cells were maintained under feeder-cell free conditions in mouse embryonic fibroblast-conditioned medi- um (MEF-CM), supplemented with 8 ng/ml of recombinant human basic fibroblast growth ... See full document

12

Human fetal liver stromal cells that overexpress bFGF support growth and maintenance of human embryonic stem cells.

Human fetal liver stromal cells that overexpress bFGF support growth and maintenance of human embryonic stem cells.

... and self-renewal [22,35]. The fact that MEF and human feeder cell lines secrete varying amounts of bFGF may partially explain why different batches of MEFs and human feeder cell lines ... See full document

12

ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells.

ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells.

... media from days 1–8 of differentiation, when the expression level of endogenous Isl1 was first detected (day 1) and reached its maximum level (day ...addition of rISL1 with muscular ... See full document

11

Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.

Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.

... status of source cells within preimplantation embryo affects phenotype and transcriptional profile of derived hESC ...One of potential hurdles in performing such an analysis is the presence ... See full document

11

Albumin-associated lipids regulate human embryonic stem cell self-renewal.

Albumin-associated lipids regulate human embryonic stem cell self-renewal.

... expression of previously described pluripotency markers such as ALP, TRA-1- 60, Oct4 and Nanog, it remained to be proven that these cells are really pluripotent in the sense that they are capable of ... See full document

10

Making human neurons from stem cells after spinal cord injury.

Making human neurons from stem cells after spinal cord injury.

... state of stem cell transplantation for ...implanting embryonic NSCs after SCI show that these cells can make oligodendrocytes effectively in vivo; however, neuron production is notably ...for ... See full document

3

Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.

Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.

... neural induction media containing DMEM/F12 with Glutamax, 16NEAA, 16N2 and FGF2 (20 ng/ml) prior to attachment on cell culture plates coated with ...population of NSCs, rosettes were manually isolated using ... See full document

11

Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.

Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.

... CHA Stem Cell Institute, Seoul, Korea), HSF-6 (established at University of California, San Francisco, CA, USA), and H9 (WiCell, Madison, WI, USA) were maintained as described previously ...layer of ... See full document

14

Microencapsulation technology: a powerful tool for human embryonic stem cells expansion and cryopreservation

Microencapsulation technology: a powerful tool for human embryonic stem cells expansion and cryopreservation

... use of mEFs, it is not entirely understood the purpose of growing hESCs on feeder cell ...feeder cells provide a suitable attachment substrate and important soluble factors for the maintenance ... See full document

76

Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.

Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.

... over-activation of EGF signaling in vivo biases cortical progenitors to astrocyte lineage and reduces NSC self-renewal in the adult SVZ where as in vitro propagation of NSCs with EGF yields more glia ... See full document

11

Xinghui Song1 , Yanwei Li1 , Xiao Chen2

Xinghui Song1 , Yanwei Li1 , Xiao Chen2

... pogenesis of primary human preadipocytes (phPA) through ...for human adipo- genesis in the absence of mitotic clonal ...phosphorylation of ERK1/2 in early differ- entiation and the ... See full document

8

Chemically-Induced RAT Mesenchymal Stem Cells Adopt Molecular Properties of Neuronal-Like Cells but Do Not Have Basic Neuronal Functional Properties

Chemically-Induced RAT Mesenchymal Stem Cells Adopt Molecular Properties of Neuronal-Like Cells but Do Not Have Basic Neuronal Functional Properties

... presence of protease inhibitors (sodium pyrophosphate, sodium fluoride, phenylmethanesulfonyl fluoride, pepstatin A, aprotinin, leupeptin, antipain, benzamidine - all from ...micrograms of protein ... See full document

11

Variations of X chromosome inactivation occur in early passages of female human embryonic stem cells.

Variations of X chromosome inactivation occur in early passages of female human embryonic stem cells.

... expression of the paternal allele suggesting skewed XCI with six out seven X-linked genes exhibiting mono-allelic expression from the paternal ...probability of having maternal or paternal allele ... See full document

9

Secondary prevention of type 1 diabetes mellitus: stopping immune destruction and promoting ß-cell regeneration

Secondary prevention of type 1 diabetes mellitus: stopping immune destruction and promoting ß-cell regeneration

... results from a cell-mediated autoimmune attack against pancreatic ...identification of ß-cell precursors have been made not only with the aim of understanding the physiology of islet ... See full document

10

Musashi2 is required for the self-renewal and pluripotency of embryonic stem cells.

Musashi2 is required for the self-renewal and pluripotency of embryonic stem cells.

... self-renewal of ESC and induces their ...extent of differentiation and the loss of self-renewal capacity correlates with the extent to which Msi2 levels were ...knockdown of Msi2 ... See full document

11

Exploring cell reprogramming techniques for Angelman Syndrome disease modelling

Exploring cell reprogramming techniques for Angelman Syndrome disease modelling

... Neuronal cells derived from direct conversion provide a novel platform for diverse applications, including disease modelling (Pfisterer et al, ...speed of conversion, possibility to generate ... See full document

63

Início e manutenção da inativação do cromossomo X em células humanas

Início e manutenção da inativação do cromossomo X em células humanas

... (human embryonic stem cells – hESCs) em 1998 permitiu novas possibilidades de estudo da ICX, pois a maioria dos trabalhos procurou esclarecer o mecanismo da ICX no modelo ... See full document

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