Top PDF Validation of commonly used reference genes for sleep-related gene expression studies
Validation of commonly used reference genes for sleep-related gene expression studies
... additional sleep recovery period of 24 hours ...were used and an additional group of 8 animals was included for a total sleep deprivation of 6 hours ...water of ~1 cm ...
8
Validation of suitable reference genes for expression studies in different pilocarpine-induced models of mesial temporal lobe epilepsy.
... significance of the results obtained for reference genes, we conducted a relative expression analysis of the Gfap gene, whose pattern is already described for the PILO injected ...
9
Reference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus Pathosystems.
... be used in combination as reference genes in the forthcoming expression qPCR analysis of target genes for ‘Santa Clara’- and ‘LAM 157’-ToCMoV interaction ...two genes ...
17
Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
... Reference genes are commonly used for normalization of target gene expression during RT-qPCR ...housekeeping genes or reference genes have been ...
8
Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... stable expression levels [16,17]. These reference genes include SAND family protein (SAND), protein phosphatase 2A (PP2A), TIP41-like family protein (TIP41), F-box/kelch-repeat protein (F-box), ...
10
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... one of several members of a small family of globular proteins, and is the major building block of microtubules in almost all eukaryotic cells, exhibited the most stable expression in ...
9
Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas)
... Cytokine gene expression research has been conducted in both free-ranging and human- cared ...cytokine gene expression and pollutants in free-ranging cetaceans can make these mammals useful ...
14
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... ideal reference gene has stable expression, regardless of experimental conditions applied to the ...number of studies have shown that expression of reference ...
7
Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues.
... component of a reliable gene expression analysis based on qRT-PCR ...use of one or more reference genes as internal controls is commonly accepted as the most appropriate ...
16
Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... instability of petroleum sources and threat of global climate change have incited a push towards locally produced renewable energy ...conversion of renewable biologic materials to liquid or gaseous ...
12
Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without Kranz anatomy
... measurement of gene expression, which depends on the stability of the reference gene used for data ...tolerance- related genes and compare relative ...
24
Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.).
... one of the most popular, high-throughput and sensitive technologies available for quantifying gene ...normalisation of gene-of-interest data with reference genes that are ...
18
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization ...
10
Unstable Expression of Commonly Used Reference Genes in Rat Pancreatic Islets Early after Isolation Affects Results of Gene Expression Studies.
... Committee of the Institute for Clinical and Experimental Medicine and Ministry of Health of Czech Republic (Permit Number: 34/2012; ...total of 38 rats were used in this ...pancreata ...
18
Identification of Suitable Reference Genes for Gene Expression Studies of Shoulder Instability
... deformation of the glenohumeral capsule. Gene expression analysis may be a useful tool for increasing the general understanding of capsule deformation, and reverse-transcription quantitative ...
9
Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs
... Reference genes have to be validated and consistently expressed under various ...Widely used reference genes for expression analyses include GAPDH, ACTB, and HPRT (Blaha et ...
19
Virus-Induced Gene Silencing Using Tobacco Rattle Virus as a Tool to Study the Interaction between Nicotiana attenuata and Rhizophagus irregularis.
... We used genes known from other plant-AMF interactions and genes that have not been described earlier for ...up-regulation of the probable N. attenuata genes ABC trans- porter A, ...
24
In vivo response to methotrexate forecasts outcome of acute lymphoblastic leukemia and has a distinct gene expression profile.
... characteristics of the ALL cells that make them resistant are ...component of most treatment protocols for newly diagnosed ...treatment of methotrexate-resistant ...response of newly diagnosed ...
11
Identification of reliable reference genes for quantitative gene expression in the Mozambique tilapia, Oreochromis mossambicus
... samples of total RNA extracted from tilapia oocyte were treated with the MicroPoly(A)Purist™ Kit (Ambion, Applied Biosystems) for isolation of poly(A) RNA according to the manufacturer’s instructions using ...
107
Over-expression of VvWRKY1 in grapevines induces expression of jasmonic acid pathway-related genes and confers higher tolerance to the downy mildew.
... involvement of jasmonic acid in grapevine resistance to downy mildew is also supported by previous ...up-regulation of several WRKY genes as well as JA biosynthesis-related genes (13- ...
8
