One-step
One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens.
... The need for one-step room temperature preservation of phosphoproteins and histomorphology transcends diagnostic pa- thology to the broader research community. Preservation of phosphoproteins and tissue ...
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ONE-STEP PURIFICATION AND CHARACTERIZATION OF CELLULASE-FREE XYLANASE PRODUCED BY ALKALOPHILIC BACILLUS SUBTILIS ASH Ashwani Sanghi
... a one-step purification procedure so as to reduce the enzyme ...single step purification and characterization of an extracellular cellulase-free xylanase from an alkalophilic and moderately ...
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Estimation of crop water requirements: extending the one-step approach to dual crop coefficients
... a one- step approach based upon a Penman–Monteith type equation ...the one-step approach can be inferred from the crop coefficients (K cb and K e ) in ...
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One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil
... The real-time RT-PCR reaction set-up was carried out using an automated workstation (Janus, PerkinElmer). HCV detec- tion was performed by a duplex, one-step, real-time RT-PCR, in a 50 L total volume ...
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ONE-STEP EXTRAPOLATION METHOD USING CHEBYSHEV’S RECURRENCE RELATION APPLIED TO SEISMIC MODELING
... RESUMO. Neste trabalho, mostra-se que a soluc¸˜ao da equac¸˜ao de onda de primeira ordem com um campo de onda anal´ıtico, usando um esquema de diferenc¸as- finitas no tempo, segue exatamente a relac¸˜ao de recorrˆencia ...
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Canine distemper virus detection by different methods of One-Step RT-qPCR
... The One-Step RT-qPCR (System A) and One-Step RT-qPCR combined with NESTED- qPCR (System B) were compared using the RT- qPCR kit I, which showed better dissociation curves, reaction time and CT ...
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Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography.
... heterogenous genes in E. coli and provide an alternative approach to increase the expression level of some target genes. In this case, to purify the target proteins conveniently, we try to coexpress protease TEV with the ...
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Centrifuge modeling of one-step outflow tests for unsaturated parameter estimations
... Since it is impractical to conduct one-step gravity driven tests that fulfill the afore- mentioned first two conditions suggested by Kool et al. (1985) (for example, several days of observation of drainage ...
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Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses.
... multiplex one-step real-time qRT-PCR, the cross-reactivity of the monoplex primers/probe was examined first using all the in vitro transcribed viral RNA standards with the concentration of 10 6 copies/ m L, ...
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A One-step Analytical Approach for Springback Compensation in Channel Forming Process
... Abstract— Springback is a very important factor to influence the quality of sheet metal forming. Accurate prediction and controlling of springback is essential for the design of tools for sheet metal forming. Several ...
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Engineering more stable, selectable marker-free autoluminescent mycobacteria by one step.
... In our previous study, we demonstrated that the use of the autoluminescent Mycobacterium tuberculosis as a reporter strain had the potential to drastically reduce the time, effort, ani- mals and costs consumed in ...
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A one-step miniprep for the isolation of plasmid DNA and lambda phage particles.
... Crude lysates of alkali minipreps cannot be used for downstream applications because of their low DNA concentration, SDS content and acidic pH. NID minipreps are free of these shortcomings, leading us to examine whether ...
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One step forward: contrasting the effects of Toe clipping and PIT tagging on frog survival and recapture probability
... than one, into survival esti- ...probability, one could conclude through the use of return rates, that toe clipping reduces survival when in fact only recapture probability is ...
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ARDS definitions in children: one step forward
... The Brazilian Pediatric ARDS Study Group 6 performed a prospective, multicentre cohort study from March to September of 2013, which aimed: (1) to evaluate the preva- lence of ARDS; (2) t[r] ...
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Development of regulatory T cells in the human thymus : one step beyond =desenvolvimento de células T reguladoras no timo humano
... The human thymus may thus concomitantly produce two populations of CD8SP cells expressing CD103, with regulatory and effector phenotypes, able to migrate to mucosal tissues, where the [r] ...
168
Nanocapsule-coated microparticles prepared in one step: characterization and in vitro drug release profiles evaluation.
... Os perfis de liberação foram analisados matematicamente através de métodos modelo-dependentes, para se determinar a velocidade de liberação da indometacina e aplicando-se, pela [r] ...
7
Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
... salt step, ionic strength of the buffer was decreased to 50 mM phosphate buffer (pH ...and step detergent gradient from zero to 1% of Triton X-100 in 50 mM sodium phosphate buffer, with 3 ...wash ...
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Phytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.
... although one may speculate that the PHCer may be involved in more tightly binding of lipid-protein or lipid-lipid complexes at the membrane interface due to the additional OH group ...
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Rev. Soc. Bras. Med. Trop. vol.49 número3
... FIGURE 1 - Melting peaks and ampliication curves of real-time RT-PCR for mosquito-borne Flavivirus using four different kits. A). Ampliication curve using Power SYBR Green PCR Master Mix (Applied Biosystems, Foster City, ...
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Braz. Dent. J. vol.24 número3
... a one-step self-etching adhesive (OSSE), and a simplified ethanol-wet bonding technique (EWBT) prior to the application of a composite resin in non-carious cervical ...
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