Real time PCR
Quantitative Real Time PCR
... 2004). Real-time PCR was performed with BioRad CFX96 Touch (BioRad, USA) using optical grade ...The PCR reaction was performed on a total volume of 25 µL using the iQTMSYBR Green Supermix ...
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Development of a Real-time PCR test for porcine group A rotavirus diagnosis
... a PCR assay for the VP1 gene of RV, in agreement with our finding of accurate de- tection in Real-Time PCR, where C t values between ...
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Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
... 1). Real-time PCR assays were performed with the two primer pairs and probes simultaneously, in ...Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA), ...Cycler ...
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Different methods of real-time PCR for detection of pseudorabies virus
... A growing number of real time PCR (qPCR) chemistries are available to diagnostic laboratories. There are differences in performance of these methods concerning costs, detection limits, specificity ...
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Eprobe mediated real-time PCR monitoring and melting curve analysis.
... forgoing PCR experiments a strong hook-like shape of the amplification curves (Figure ...the PCR when using a Taq DNA polymerase having a 5 9–39 exonuclease ...of PCR product did no longer change at ...
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Multiplex real-time PCR diagnostic of relapsing fevers in Africa.
... single real-time PCR experiment was performed on Roche Lightcycler (RocheDiagnostic, Maylan, ...Quantitative PCR Master mix (Quantitec, Qiagen) and the volume was adjusted to 15 m L by adding ...
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Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
... Faster diagnoses of human TB is of significant clini- cal importance. There are commercial methods available to detect M. tuberculosis, but these techniques rely on multi- ple probes or detect Mycobacterium tuberculosis ...
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Quantitative analysis of the dystrophin gene by real-time PCR
... (single PCR product without any ampliication of non-speciic products is seen as one single sharp peak on the melting curve or one single band on agarose gels) showed that no detectable non-specif- ic products were ...
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Development of Real Time PCR Meat detection kits and technology market transfer
... The fluorophore-labelled oligonucleotides are small single DNA strand with attached fluorophore, which is a small molecule capable of emitting fluorescence. The fluorophore molecules can be classified into donor or ...
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Neurotoxoplasmosis diagnosis for HIV-1 patients by real-time PCR of cerebrospinal fluid
... by real time ...patients. Real time PCR-Multiplex was applied to these patients’ cephalorachidian liquid with a specific ...by real time Multiplex of cephalorachidian ...
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Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... various PCR-based molecular ...multiplex real-time PCR (Taq- Man) assay using a probe-primer set for detection of the three known species of Liberibacter causing ...
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Avaliação laboratorial da doença residual mínima na leucemia mielóide crônica por Real-Time PCR.
... A leucemia mielóide crônica (LMC) representa 15% das leucemias e apresenta três fases: crônica, acelerada e crise blástica. A partir da análise citogenética, pode ser identificado o cromossomo Philadelphia, ...
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Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses.
... Multiplex PCR methods are attractive to clinical laboratories wanting to broaden their detec- tion of respiratory viral pathogens in clinical ...singleplex real-time PCR assays targeting ...
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Detecção molecular de hantavírus pela técnica de real-time PCR em amostras de roedores...
... de Real-time PCR utilizando o sistema SYBR Green, realizamos uma nova amplificação em outra região do gene S e do gene M (Figura 15) utilizando outros primers específicos (Moreli et ...de PCR ...
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REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... The real-time PCR assay we developed was approximately 1000-fold more sensitive than conventional PCR assays (gel electrophoresis after PCR) for detection of ...by ...
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A real-time PCR antibiogram for drug-resistant sepsis.
... The real-time PCR antibiogram, described herein, combines universal phenotypic susceptibility testing with the rapid diagnostic capabilities of ...standard real-time PCR ...
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Comparison of real-time PCR and conventional PCR for detection of
... quantitative real-time PCR ...conventional PCR include speed, reproducibility and quantitative ...conventional PCR in diagnostic ...of real-time PCR in highly ...
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Real-time PCR in infectious uveitis as an alternative diagnosis
... Purpose: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness ...
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RNA extraction, RT and Real-Time PCR (qPCR)
... Bird identification and biometric information were entered in spread sheets for further analysis, if necessary. The spreads heet included biological status: new bird, recaptured, recovered; age:adult, young, nestlings, ...
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Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli
... FIG 1 Real-time PCR assay simultaneously detects nine different DEC viru- lence genes. Data from individual reaction mixtures, each containing DNA from ETEC, EAEC, STEC, EPEC, EIEC, or DAEC strains, ...
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