Real time quantitative PCR (qPCR)
Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.
... mechanisms. Real-time quantitative PCR (qPCR) is a useful technique for acquiring the gene expression pattern of a number of selected genes due to its high sensitivity, specificity and broad ...
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TaqMan MGB probe fluorescence real-time quantitative PCR for rapid detection of Chinese Sacbrood virus.
... fluorescence real-time quantitative PCR was ...fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods ...
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Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
... by real-time quantitative PCR (real-time ...a real-time Q-PCR using the SYBRGreenH method, a primer set already used to study the genetic structure of soil ...
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Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... by real-time qPCR (Table ...that real-time qPCR based on short amplicons (70– 250 bp) was independent of RNA integrity and therefore give more accurate results than long ...amplicons. ...
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Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... de PCR quantitativo em tempo real (RT-qPCR) genes para serem utilizados como referência em estudos de expressão gênica em soja, em ensaios de estresse ...
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Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria
... lower concentration that could be used as a positive con- trol in PCR assays. Since analytical sensitivity most often resides below the linear range, a cloned cysteine protein- ase gene was used to generate P. ...
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Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient
... tempo real (qPCR) do DNA do espermatozoide e dos embriões bovinos produzidos in vitro resultantes pela PCR ...a PCR foi realizada para a identificação genética do sexo dos ...
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Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... levels. Quantitative Real-Time PCR (qPCR) assays based on 16S rDNA genes are widely used in the detection of ...first time a set of qPCR primers based on the conserved 16S rDNA gene, ...
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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive technique for gene expression analysis, depends on the stability of the reference genes used for data ...
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Comparison of real-time PCR and conventional PCR for detection of
... with quantitative real-time PCR ...conventional PCR include speed, reproducibility and quantitative ...conventional PCR in diagnostic ...of real-time ...
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... identify the catalase family genes in the melon genome, the Hidden Markov Model of catalase family (PF00199) was downloaded from the Pfam database (http://pfam.sanger.ac.uk/) and used as a query to search against the ...
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Accessing Planktothrix species diversity and associated toxins using quantitative real-time PCR in natural waters
... costly, time consuming and highly operator dependent. So, species-specific real-time PCR analysis is also useful in cyanobacteria ...common real- time PCR methods for ...
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Fibroblast growth factor receptor 1 amplification in non-small cell lung cancer by quantitative real-time PCR.
... FISH analysis is laborious, technically complex, and reader dependent. All are characteristics that limit its clinical applicability. We therefore designed an assay that evaluates FGFR1 gene amplification by ...
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The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas
... time PCR and SQ-PCR). A differential PCR for amplification of the MYCN gene was initially developed by Gilbert et ...described PCR-based approaches, including Southern ...relative ...
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Quantitative Real Time PCR
... DNA was extracted from cecal samples and pure cultures by using the QIAamp DNA Stool Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s protocols. The extracted DNA was stored at -20°C until use. The ...
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Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
... pure PCR template preparation kit (Roche Applied Science), and for a third aliquot the auto- mated method with the MagNA Pure LC DNA Isolation Kit – large Volume (Roche Applied Science) and the MagNA Pure LC ...
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Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... In order to validate the use of the genes selected by geNorm and NormFinder algorithms as reference genes, we examine the effect of using different HKGs on the qRT-PCR-determined expression profile of putative ...
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Key words: Biofilm formation, biosurfactant, gtfBC, Lactobacillus acidophilus, real-time RT PCR,
... conditions. Quantitative and qualitative evaluations performed on the extracted RNA by spectrophoto-metrically (biophotometer, Eppendorff, Rs 232-C, Germany) and Agarose-gel ...
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Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
... to real-time PCR assay. However, real-time PCR assay and LAMP reaction have shown superior sensitivity compared to conventional PCR used to detect ...and ...
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Multiplex real-time PCR diagnostic of relapsing fevers in Africa.
... single real-time PCR experiment was performed on Roche Lightcycler (RocheDiagnostic, Maylan, ...L Quantitative PCR Master mix (Quantitec, Qiagen) and the volume was adjusted to 15 m L ...
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