[PDF] Top 20 Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
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Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
... changes in gene expression induced by abiotic stress ...nature of the technique limits the ability to accurately quantify expression ...This, in conjunction with the low throughput ... See full document
10
Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... analysis of gene expression [16, 23, 24]. However, to the best of our knowledge, a systematic study on endogenous control genes expression under different stress conditions has ... See full document
20
Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many studies involving gene expression ...analysis. In ... See full document
11
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... suitable reference gene for expression normalization in ...involved in the process of protein translation, eIF-4a had the same performance as eEF-1a in most experimental ... See full document
10
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars
... stability genes for quantitative polymerase chain reaction in sugarcane were described by ISKANDAR et ...the reference gene recommended for gene expression studies, with great stability ... See full document
7
Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).
... involved in citrate cycle and metabolic pathways, ...best reference gene for adult stage and insecticide ...stable in larvae stage followed by EF1a and ...experimental conditions, SDH and ... See full document
16
Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... analyses in Arabidopsis thaliana and soybean that show highly stable expression levels ...These reference genes include SAND family protein (SAND), protein phosphatase 2A (PP2A), TIP41-like ... See full document
10
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... accurate quantitative real-time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene(s) is ...stability of its reference genes have not ... See full document
9
Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut
... stability of the candidate refer- ence genes, the expression profile of a gene induced by water deficit was analyzed using two reference genes selected in this ...library ... See full document
11
Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without Kranz anatomy
... transcription quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful analytical technique for the measurement of gene expression, which depends on the stability of the ... See full document
24
Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... 11 genes used for the analysis showed high expression stability and presented an M value lower than the cutoff established by Vandesompele et ...stable reference genes were not identical in ... See full document
12
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.
... transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression ...application of RT-qPCR requires accurate normalization though the ... See full document
14
Importance of suitable reference gene selection for quantitative RT-PCR during ATDC5 cells chondrocyte differentiation.
... influence of reference genes on the expression profiles of target genes, cells cultured in GM, IM and CM were harvested at day 7 followed by qPCR ...data of fold change ... See full document
7
Application of time-series analysis for prediction of molding sand properties in production cycle
... nature of manufacturing processes and permits prediction of future values of the process parameters or production results on the basis of the past data, recorded in regular ...problems ... See full document
5
Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.
... case of endothelial cells of the blood-brain barrier (BBB) of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases ... See full document
22
Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer
... that quantitative analysis of mRNA can be achieved by several RT-PCR approaches, which can be divided into comparative and absolute quanti- tative PCR (Q-PCR), both of ... See full document
7
GENETIC VARIABILITY OF CULTURED PLANT TISSUES UNDER NORMAL CONDITIONS AND UNDER STRESS
... observed in the somaclones. All changes were found in noncoding sequences and were induced by different molecular events, such as the insertion of long terminal repeat transposon, precise miniature ... See full document
1
Real Time PCR and Importance of Housekeepings Genes for Normalization and Quantification of mRNA Expression in Different Tissues
... time PCR technique is effective to measure the absolute transcript and provides valuable quantitative information on gene expression from different sources and samples (Peters et ...sensitive ... See full document
12
Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing.
... energies of tRNA ...precursors of these miRNAs required 75–343 nt, with a majority of the identified miRNA precursors (88%) requiring 75–188 nt, similar to what had been observed in ... See full document
10
Parental genome dosage imbalance deregulates imprinting in Arabidopsis.
... used quantitative RT-PCR to investigate the effect of increased parental dosages in crosses between tetraploid and diploid ...imprinted genes studied are highly expressed and ... See full document
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