[PDF] Top 20 Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
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Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... real-time PCR (qRT-PCR) is now globally used for accurate analysis of tran- scripts levels in ...quantification of transcripts, identification of the best reference ... See full document
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Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... 1-alpha) and eIF-4a (Eukaryotic initiation factor 4-alpha) in Oryza sativa; CUL (Cullin), FPGS (Folylpolyglutamate synthase), LUG (Leunig), MEP (Membrane protein) and UBCP (Ubiquitin carrier protein) ... See full document
10
Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many studies involving gene expression ...analysis. In ... See full document
11
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
... F. and col- laborators (2005), significant differences in gene expres- sion between GBM and normal white matter were found for ACTB (Student's t test, P = 0,016), GAPDH (P = 0,006), GUSB (P = 0,005) ... See full document
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Selection and Validation of Reference Genes for Accurate RT-qPCR Data Normalization in Coffea spp. under a Climate Changes Context of Interacting Elevated [CO2 ] and Temperature
... importance of the Minimum Information for Publication of Quantitative Real- Time PCR Experiments (MIQE) guidelines (Bustin et ...normalize RT-qPCR ...stability of the evaluated ... See full document
11
Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... 11 genes used for the analysis showed high expression stability and presented an M value lower than the cutoff established by Vandesompele et ...stable reference genes were not identical ... See full document
12
Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
... changes in gene expression induced by abiotic stress ...membrane and hybridized with a detectable complementary probe ...consuming and labor intensive. And although the sensitivity threshold ... See full document
10
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.
... transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression ...application of RT-qPCR requires accurate normalization though ... See full document
14
Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.
... Quantification of gene expression modulation is usually carried out by means of quantita- tive real-time reverse transcription-coupled PCR (qRT-PCR), and amplification data are nor- ... See full document
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Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues.
... aim of finding suitable reference genes for gene expression studies in EC tissue samples, we carried out a Medline search using the MeSH terms ‘‘endometrial cancer’’ and ‘‘real-time ... See full document
16
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... levels and obtain more accurate quantitative real-time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene(s) is ...Asia, and recently invaded ... See full document
9
Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... used in this study, one might wonder which one could lead to the most reliable ...ranking of reference genes [44,47]. This package uses the matrix of rank-ordered genes as an ... See full document
16
Walking towards the “hidden” role of dirigent proteins in Vitis vinifera secondary metabolism
... observation of the previous results, Fa fraction was apparently able to partially guide the reaction towards the formation of - ...viniferin. In addition, at least two other products (a and b ... See full document
208
Assessment Of NPK In Human Male And Female Urine For Its Fertilising Potential In Agriculture
... urine. In the absence of significant differences in gender pH changes, phosphorus yield might have been influenced by storage time and/or temperature ...peaked in month 4, indicating ... See full document
9
Identification of reliable reference genes for quantitative gene expression in the Mozambique tilapia, Oreochromis mossambicus
... farming in aquaculture systems [14]. Tilapias are now the second group of fish most farmed worldwide (behind carps) with an annual production of ...representative in this farming industry are ... See full document
107
Parental genome dosage imbalance deregulates imprinting in Arabidopsis.
... Real-time PCR assays were performed using a PCR Master Mix (Applied Biosystems, Foster City, ...l of RT product was used to perform each PCR ...concentration of 0.5 mM in ... See full document
8
Functional and Morphological Correlates in the Drosophila LRRK2 loss-of-function Model of Parkinson's Disease: Drug Effects of Withania somnifera (Dunal) Administration.
... 2% of the population over 60 years with an increasing incidence over age 85 ...loss of dopaminergic neurons in the substantia nigra of the midbrain leads to a deficiency of dopamine ... See full document
16
Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions.
... studies of Chinese jujube, ZjH3 was reported as the most suitable housekeeping gene for evaluating jujube fruit-bearing shoot development by semi-quantitative RT-PCR [3], whereas, its ... See full document
11
Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... comparison of the different methods could be the best way to identify the best reference genes to be ...NormFinder and BestKeeper) to evaluate our ten potential reference ...time ... See full document
9
Application of time-series analysis for prediction of molding sand properties in production cycle
... used in the present work have been obtained from a Polish grey cast iron foundry, equipped with two Disamatic molding lines (2110 Mk3 and 2110 Mk5 with a sand cooling ...months, in 39 working days ... See full document
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