[PDF] Top 20 MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND

... DNA amplification was carried out under the following conditions: 94 °C for 10 min and then 35 cycles at 92 °C for 30 ...followed by a final extension at 72 °C for 5 ...40-fold in sterile water. One ... See full document

... analysis in jet ...29]. By using NGS, we could determine the variation in the microbial community and the microbial characteristics in jet fuel in different stages of production and ... See full document

... Advances in molecular biology have seen the establishment of the loop-mediated isothermal amplification (LAMP) assay, originally described by Notomi et al (Notomi et ... See full document

... of malaria elimination and eradication as outlined by the ...available in reference laboratory settings, the cur- rently available diagnostic tools outside referral settings for the detection of ... See full document

... described by de Souza et ...procedures in third level laboratories or national research centers, which may not be practicable at primary health care ...decrease in sensitivity ... See full document

... specimens by culture assay and PCR, no ...positive amplification of ...LAMP in human stool, serial 10-fold dilutions of a mid-log-phase culture of ...grown in BHI were prepared in ... See full document

... 2000). In the diagnosis of vibriosis in seabass, the LAMP assay can be completed in an hour, and this was faster than either conventional or nested-PCR ...assays. In addition, the speed ... See full document

... utilized in this study were de-identified prior to laboratory processing and ...participated in prospective, population-based surveillance for childhood men- ingitis from 1999 to 2002 [16, ...recorded ... See full document

... Province, in 2008. Of these 24 patients, 6 were confirmed to have Q fever by serological analysis (a 4-fold increase in the IgG titer of the antibody against I phase antigen of ...used in the ... See full document

... successful amplification of ...assay by shortening the duration (Figure 2) and increasing sensitivity of the ...DNA amplification is preceded by strand separation under isothermal ... See full document

... example by detect- ing anti-HIV antibodies in oral samples—however, the nucleic acid-based method presented here has additional potential ...[16]. In one implementation of the technology, cell phones ... See full document

... acid amplification via LAMP has several cost advan- tages over ...acid amplification. Reactions are carried out in single tubes, and results can be visualized under UV ...light. In order to ... See full document

... using loop mediated isothermal amplifica- tion (LAMP) is growing in popularity because of its practicality, speed and usefulness in laboratories and clinical settings ...favorably ... See full document

... microscopy in comparison with culture as the gold ...testing in series and parallel were also compared against the culture ...compared in series (LAMP performed only if smear positive and considered ... See full document

... determined by agarose gel electrophoresis, by the use of spectrophotometric equipment to measure turbidity, by naked eye for the presence of a white precipitate derived from magnesium pyrophosphate, ... See full document

... infections in diverse ani- mal species and occasionally in humans. In this study, we described the establishment of a simple, sensitive and cost-efficient loop-mediated ... See full document

... mouse in a defined time-point p.i., the sera samples were obtained by allowing the blood to clot for 15–30 minutes at room temperature and removing the clot by centrifuging at 1,000–2,0006 g for 10 ... See full document

... TB diagnosis to areas with high disease ...employed in over 100 high burden countries [1], but its use is still limited by cost of support and maintenance ...[7]. Isothermal ... See full document

... gastroenteritis in humans via the ingestion of raw or undercooked contaminated seafood, and early diagnosis and prompt treatment are important for the prevention of ...diseases. In this study, a ... See full document

... (Sigma-Aldrich, St. Louis, MO), 2 mM of each deoxynucleoside triphosphate (dNTP)(Promega, USA), 40 pMol of FIP and BIP primers, 5 pMol of F3 and B3 prim- ers, 20 pMol of LF and LB primers, 8 U of Bst DNA poly- merase, 2 ... See full document