[PDF] Top 20 NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION
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NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION
... Leishmania infantum causes visceral leishmaniasis (VL) in the New ...diagnosis of VL is confirmed by parasitological and serological tests, which are not always sensitive or ...design ... See full document
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Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
... The primers used to detect Leishma- nia ...PCR reaction mixture (50 µl total volume) con- sisted of PCR buffer 1X (10mM Tris-HCl, pH ...U/µl of Taq DNA polymerase (Perkin-Elmer), 200 µM ... See full document
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Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray.
... genotyping of 15 different hrHPVs and two low-risk HPVs ...amplification of the viral L1 region using an improved set of type-specific PGMY primers (modified from [21]) and subsequent ... See full document
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Detection of DNA from Leishmania (Viannia): accuracy of polymerase chain reaction for the diagnosis of cutaneous leishmaniasis.
... diagnosis of CL involves immunological methods and optical detection of the parasite, al of which have ...development of severe forms of mucosal disease, and to estimate the ... See full document
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Analysis of Respiratory Syncytial Virus in Clinical Samples by Reverse Transcriptase-Polymerase Chain Reaction Restriction Mapping
... Comparison of RNA-PCR sensitivity with the plaque test assay and IFI - The “Long” strain was cultured on HEp2 cells in flasks with a surface area of 25 cm 2 , containing 5 ml of MEM, 5% fetal calf ... See full document
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Polymerase chain reaction (PCR)
... objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the ... See full document
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Caracterização clínica e histopatológica e tipagem do papilomavírus humano das verrugas vulgares nos receptores de transplante renal
... prevalence of skin lesions caused by the human papillomavirus (HPV) is high in kidney transplant ...recipients of kidney transplants with a diagnosis of common warts were eva- ...HPV detection ... See full document
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DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis
... amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous ... See full document
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Enhanced detection and serotyping of <I>Streptococcus pneumoniae</I> using multiplex polymerase chain reaction
... Surveillance of pneumococcal serotypes should be continued in order to monitor changes in serotype distribu- tion necessary for the pneumococcal vaccine ...way of isolating and serotyping ...26% of ... See full document
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Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
... variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in ...profile of patients affected by different DENV serotypes or ZIKV may be similar, ... See full document
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Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction
... amount of each pair of primers in MAS-PCR was balanced to achieve acceptable amplification of all target ...MAS-PCR reaction, a standard 35 µL final reaction was ...8 ... See full document
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Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil
... cause of gastro- enteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical ... See full document
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... set using the SYBR Green I method and including specially designed primers that anneal in highly conserved region of the gly- coprotein of ...The primers were able to amplify the ... See full document
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DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
... PCR reaction mixture (final volume 25 µL) was composed of 0.5 µM of each of the primers (Invitrogen Life Technologies, São Paulo, Brazil), ...DNA Polymerase, (Invitrogen, ... See full document
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J. Bras. Patol. Med. Lab. vol.50 número4
... al. Detection and quantitation of HPV in genital and oral tissues and luids by real time ...use of general primers in the polymerase chain reaction permits the ... See full document
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Valéria CS Pinheiro+ , Wanderli P Tadei, Patrícia MSS Barros, Pedro FC Vasconcelos, Ana Cecília R Cruz
... sequences of the E gene region (viral enve- lope), utilizing specific primers P992 (sense) and CP2550 (nonsense) (Lanciotti et ...fidelity polymerase (PlatinumTaQ and Superscript II – Invitrogen, San ... See full document
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Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction
... onset of disease. At that time demonstration of anti-dengue IgM antibody was a good marker to confirm the clinical and epidemiological diagno- ...by using the immunoperoxidase technique (data not ... See full document
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Polymerase Chain Reaction (PCR) Identification of Terverticillate
... Phylogenetic tree analyses showed that the studied terverticillate penicillia were prominently divided to four clades. One of them was included as P. chrysogenum, Penicillium species and closely related isolates ... See full document
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ALT-C, A DISINTEGRIN-LIKE CYS-RICH PROTEIN FROM Bothrops alternatus, INCREASES SKELETAL MYOBLAST VIABILITY Mesquita-Ferrari RA (1), de Moraes CK (2), Micocci KC (2), Selistre-de-Araújo HS (2)
... ALT-C was reported to induce in vitro VEGF expression by fibroblasts, but not by HUVEC (18). However, in the present study VEGF expression was not detected either at mRNA or protein level, suggesting that this effect may ... See full document
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Short Communication PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil
... for Leishmania were subjected to PCR with internal canine ...Specific primers for β-actin Forward (5′-CTTCTACAACGAGCTGCGCG-3′) and Reverse (5'-TCATGAGGTAGTCGGTCAGG-3') were used (GenBank: ...The ... See full document
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