[PDF] Top 20 Reactivation of latent bovine herpesvirus type 5 in cattle with

... ingestion of ammonium sulphate Steer 2, which had been inoculated with BoHV-5 at the start of the experiment (118 days before), showed loss of ...samples of frontal cortex and ... See full document

... role in the development of respiratory disease in cattle herds, especially in dairy farms (KIMMAN et ...BRSV in different countries often reveal prevalences higher than 40% ... See full document

... ability of Wharton’s jelly cells from a variety of animal species to differentiate into neurons and/or glial cells in vitro ...treatment with bFGF (basic fibroblast growth factor), ... See full document

... safety of the recombinant virus was further investigated by IM inoculation of 8-month-old calves ...virus in secretions and remained healthy during the clinical ...monitoring. Reactivation ... See full document

... 3, 5 and 7) but not in mock-infected cells (Figure 1, lanes 2, 4, 6 and ...react with nitrocellulose-bound viral pro- teins (data not ...specificity of these mAbs, MDBK cell monolayers ... See full document

... increase in CSF protein concentration was not characterized as a consistent ...increase in the CSF protein concentration could be observed in nine naturally infected calves (Table ...end of ... See full document

... (tk)-deleted bovine herpesvirus 5 (BoHV-5tkΔ) was previously shown to establish latent infection and reactivate - even poorly - in a sheep model (Cadore et ...reactivate in ... See full document

... BCL-2 bovine sequences (Applied ...ampliication of 40 cycles at 95°C (15 s) and 60°C (60 ...replicates of each sample to ensure representative and accuracy ...expression of the bovine ... See full document

... detected in sera from animals infected with both the wild type and recombinant BoHV-5 (Figure ...levels of neutralizing antibodies detected until day 60 pi, when the Dx treatment took ... See full document

... and reactivation (3, ...rise in specific IgM and IgA antibodies, followed by IgG1 and IgG2 responses (3, 5, 7, ...degree of stimulation of humoral immune response, as estimated by the ... See full document

... (TK) of alphaherpesviru- ses is a product of an early gene required for viral DNA replication in neurons, but is not essential for viral replicati- on in epithelial cells or in cell ... See full document

... compared with expected fragments based on previously reported BoHV-1 sequences ...cloned in plasmid pCR ...One of the clones containing the 5´gE flanking region was digested with ... See full document

... challenge with a wild type BoHV-5 strain. The in vitro properties of the triple deletion mutant indicate that it is attenuated: the small plaque phenotype of the triple deletion ... See full document

... Bovine herpesvirus 5 (BoHV-5) is an important cause of meningoencephalitis in young and adult ...etiology of neurological disturbances in cattle makes the ... See full document

... observation of increased antibody levels in animals fed Sb is in agreement with results obtained in mice and sheep supplemented with Sb and vaccinated against Escherichia coli, ... See full document

... to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may ... See full document

... occur in a low frequency in the virus population ...selected in vitro by multiplying the virus in the presence of the drug ...enzyme with an altered substrate specificity; ii) ... See full document

... addition of IGF-1 increases the monolayer viability, which had allowed more virus production after 120 ...30% of the respective monolayers by addition of IGF-I to the media with no replacement ... See full document

... and with reference strains of other related viruses (Suid Herpesvirus type 1 [acces- sion number ...Caprine Herpesvirus type 1 [accession number ...detected in the four ... See full document

... case of meningoencephalitis caused by bovine herpesvirus-5 (BoHV-5) in cattle in Rio Grande do Sul, Brazil, occurring in 2002-2004, are ...risk of ... See full document