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[PDF] Top 20 Revisiting heterochromatin in embryonic stem cells.

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Revisiting heterochromatin in embryonic stem cells.

Revisiting heterochromatin in embryonic stem cells.

... ES cells and neurons, and mutually exclusive with active (H3K4me2) and other repres- sive chromatin marks ...domains in neurons, mostly over the bodies of transcribed genes, as well as loss of H3K9me2 from ... See full document

3

Normal DNA methylation dynamics in DICER1-deficient mouse embryonic stem cells.

Normal DNA methylation dynamics in DICER1-deficient mouse embryonic stem cells.

... reductions in DNA methylation sometimes seen in Dicer1 2/2 ES cells, and also seen here, are the result of a critical reduction in DNMT ...lost in ES cells lacking DICER1. These ... See full document

13

hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.

hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.

... K in the KRAB-ZFP/KAP1-based silencing pathway acting on ERVs and retroviral vectors in pluripotent stem ...8). In wt mESCs, KRAB-ZFPs recruit KAP1 in an oligomeric state, possibly as a ... See full document

32

Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts

Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts

... bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced ...murine embryonic fibroblasts ...layer. Cells were further propagated and ... See full document

8

The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method

The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method

... involved in reprogram- ming to produce iPSCs remain largely ...an in-depth analysis of the biological functions of differentially expressed genes in all 20 iPSC lines ...difference in gene ... See full document

8

In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.

In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.

... ES cells have been reported. In mice, germ cells have been generated from ES cells using monolayer culture [1], the formation of embryoid bodies (EBs) [2,3], co-aggregation with BMP4-produc- ... See full document

10

Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

... indicated in parentheses: OCT4 (F: 5 9-GAA AGG CTT CCC CCT CAG GGA A-3 9 R: 59-AAG AAC ATG TGT AAG CTG CGG-39; 460 bp; GenBank accession number NM 002701), NANOG (F: 59-ATG CAG GCA ACT CAC TTT AT-39 R: 59- TTC AGG ... See full document

13

Innate immunity in human embryonic stem cells: comparison with adult human endothelial cells.

Innate immunity in human embryonic stem cells: comparison with adult human endothelial cells.

... delay in development of innate immune sensing in the ...are in agreement with others [20] who also reported lack of responsiveness of undifferentiated mouse ESC and their differentiated derivatives ... See full document

12

Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.

Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.

... used in this study were either previously described [25] or derived with the ethics permission obtained from the NHS Lothian Research Ethics Committee ...pluripotent stem cell ...mouse embryonic ... See full document

11

Directed in vitro myogenesis of human embryonic stem cells and their in vivo engraftment.

Directed in vitro myogenesis of human embryonic stem cells and their in vivo engraftment.

... Our in vivo data suggests that the amount of culture time and the differentiation state of the cells before transplantation is of utmost importance in ensuring a viable and engraftable ...The ... See full document

10

Self-contained induction of neurons from human embryonic stem cells.

Self-contained induction of neurons from human embryonic stem cells.

... shown in this study that neurons and astrocytes can be produced efficiently from hES cells using a conditioned medium collected from either rat primary-cultured astrocytes or hES cell- derived ...hES ... See full document

8

Molecular mechanisms triggering differentiation of the embryonic endodermal stem cells

Molecular mechanisms triggering differentiation of the embryonic endodermal stem cells

... of cells that carry the cloning vector-insert DNA contruct 73 . In this study, the cloning vector used was pGEM-T 2 (Promega GmbH, ...transformed cells that are grown in the presence of the ... See full document

124

Unraveling the global microRNAome responses to ionizing radiation in human embryonic stem cells.

Unraveling the global microRNAome responses to ionizing radiation in human embryonic stem cells.

... that in mammals the primary mode of miRNA- mediated gene repression is through inhibition of translation, not direct mRNA target degradation ...observed in our previous work [24]. The repression of genes ... See full document

9

Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.

Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.

... CHA Stem Cell Institute, Seoul, Korea), HSF-6 (established at University of California, San Francisco, CA, USA), and H9 (WiCell, Madison, WI, USA) were maintained as described previously ...mouse embryonic ... See full document

14

Differentiate or Die: 3-Bromopyruvate and Pluripotency in Mouse Embryonic Stem Cells.

Differentiate or Die: 3-Bromopyruvate and Pluripotency in Mouse Embryonic Stem Cells.

... maintained in control conditions (with LIF), in the absence of LIF (differentiation control) and in the presence of two different 3BrP concentrations (25 and 50 μM) plus ...colonies in all ... See full document

17

Início e manutenção da inativação do cromossomo X em células humanas

Início e manutenção da inativação do cromossomo X em células humanas

... ICX in vitro, o que permite a identificação dos fatores e mecanismos moleculares ...(human embryonic stem cells – hESCs) em 1998 permitiu novas possibilidades de estudo da ICX, pois a maioria ... See full document

54

Regulation of PKM2 and Nrf2-ARE pathway during benzoquinone induced oxidative stress in yolk sac hematopoietic stem cells.

Regulation of PKM2 and Nrf2-ARE pathway during benzoquinone induced oxidative stress in yolk sac hematopoietic stem cells.

... pronounced in embryonic YS-HSCs than in adult BM-HSCs ...system in embryonic YS-HSCs treated with ...role in determining the biological effects of benzene-induced ROS in ... See full document

14

polarization of the public debate about embryonic stem cells in Brazil :: Brapci ::

polarization of the public debate about embryonic stem cells in Brazil :: Brapci ::

... fertilização in vitro, para os embriões congelados há três anos ou mais, no trato dos embriões considerados ‘inviáveis’, na autorização expressa dos genitores dos embriões e na proibição de destruição dos embriões ... See full document

10

Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells.

Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells.

... different stem cells into SMCs, very few evaluated the impact of the 3D environment at the geno- and phenotype of the differentiated cells ...encapsulated in a 3D collagen matrix have ... See full document

14

Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media.

Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media.

... primary cells are also routinely used in biological ...Mouse embryonic fibroblast (MEF) cells are used as feeder cells to maintain ES cells and induced pluripotent stem ... See full document

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