[PDF] Top 20 The first serine protease inhibitor from Lasiodora sp (Araneae: Theraphosidae) hemocytes
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The first serine protease inhibitor from Lasiodora sp (Araneae: Theraphosidae) hemocytes
... inhibit serine, cysteine and aspartic acid proteases, as well as ...Additionally, the best character- ized families of protease inhibitors are Kazal, Kunitz and Pacifastin ...[13–15]. Serine ... See full document
5
Purification and characterization of a serine protease from Alternaria alternata
... AEX. The alkaline serine protease from Beauveria ...steps. The first was an ammonium sulfate precipitation and the second step involved AEX using a DEAE-cellulose column ... See full document
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Tryptogalinin is a tick Kunitz serine protease inhibitor with a unique intrinsic disorder.
... uses the Basdevant et ...optionally, the search may be biased towards a desired goal by adding geometric ...on the TdPI-trypsin crystal (PDB: 2UUY), we added an 8A ˚ cutoff between Lys13 and Asp191 ... See full document
12
Clonagem, expressão e caracterização de um inibidor de tripsina presente no mosquito Aedes aegypti
... for the first time a Kazal unique domain trypsin inhibitor from the Aedes aegypti ...showed the potential presence of a Kazal-type serine protease inhibitor, in ... See full document
120
Protease inhibitors from marine actinobacteria as a potential source for antimalarial compound.
... at the Cambodia–Thailand border ...drugs. The in silico study revealed that protease inhibitors from five actinobacteria liable to show very high affinity towards the respective ... See full document
13
HATL5: a cell surface serine protease differentially expressed in epithelial cancers.
... obtained from Biochain (Newark, CA). First strand cDNA synthesis was performed with Oligo (dT) primers using a RETROscript kit according to the manufacturers’ instructions (Ambion, Life Technologies, ... See full document
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Structural and functional characterization of cleavage and inactivation of human serine protease inhibitors by the bacterial SPATE protease EspPα from enterohemorrhagic E. coli.
... are serine protease autotransporters found in enterohemorrhagic Escherichia ...to the SPATE autotransporter family and are believed to contribute to pathogenicity via proteolytic cleavage and ... See full document
12
Effect of Natural Polymorphisms in the HIV-1 CRF02_AG Protease on Protease Inhibitor Hypersusceptibility
... all the mutants, their parental wild-type virus, and the NL4-3 control virus showed similar sensitivities to AZT ...in the world- wide epidemic (8%) and the availability of an infectious ... See full document
7
Network analyses reveal pervasive functional regulation between proteases in the human protease web.
... Given the observed importance of inhibitors, we assessed the possibility of incorrect annotation of cleavages of ...inhibitors. The molecular mechanism of cysteine or serine protease ... See full document
20
REPOSITORIO INSTITUCIONAL DA UFOP: Clonagem do cDNA codificante para a toxina madura LTx5 da aranha Lasiodora sp no vetor pYES2.1/V5-His-TOPO® e expressão da proteína recombinante.
... 17 A sequencia codificante para a toxina LTx2, foi sub-clonada no vetor de expressão pET11a (Novagen). O peptídeo foi expresso em células de E. coli (BL21 DE3) e purificado por cromatografia liquida de fase reversa de ... See full document
97
Assessment and partial purification of serine protease inhibitors from Rhipicephalus (Boophilus) annulatus larvae
... of serine protease inhibitors, particularly those that prevent blood clotting and inflammatory responses during blood ...feeding. The tick Rhipicephalus (Boophlus) annulatus is an important ... See full document
7
A multifunctional serine protease primes the malaria parasite for red blood cell invasion
... of the Gag polyprotein by the virus-encoded aspartic protease during capsid assembly and budding is strictly required for virus infectivity (Kaplan et al, 1993; Pettit et al, 1994; Wiegers et al, ... See full document
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PURIFICATION AND PARTIAL CHARACTERIZATION OF A COAGULANT SERINE PROTEASE FROM THE VENOM OF THE IRANIAN SNAKE Agkistrodon halys
... 2). The total yield of this fraction was 56% that accounted for ...activity. The PT-test reached its elution peaks at 9 and 12 minutes (Figure 3). The purification procedure of AH143 is summarized in ... See full document
13
Assay of Protease Activity and Protein Content
... on the isolation and purification of fibrinolytic enzymes with potential thrombolytic applications from various sources such as fermented foods, earthworms and snake venom (Peng et ...including the ... See full document
10
Identification and characterization of fusolisin, the Fusobacterium nucleatum autotransporter serine protease.
... A serine endopeptidase of 61–65 kDa capable of damaging host tissue and of inactivating immune effectors was detected previously in ...describe the identification of this serine protease, ... See full document
13
Mauro Manuel Martinez-Pacheco1 , Alberto Flores-Garcia
... 1994). The beneficial effect of this physical chemical action in the roots of wheat, buckwheat, legumes and triticale can be interpreted by the formation of stable molecular complexes between ... See full document
8
Bowman-Birk protease inhibitor from Vigna unguiculata seeds enhances the action of bradykinin-related peptides
... 2A). The recorded polydispersity was lower than ...indicating the self-association of BTCI in different multimeric states, when in solution [32] or air-dried ...to the observed polydispersity, which ... See full document
23
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels
... derived from ion exchange high-performance liquid chromatography (HPLC) showed little overlap between vertebrate and inverte- brate ...Although the two toxins (LpTx1 and LpTx2) were isolated and sequenced ... See full document
9
A Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Mice
... blocking the endogenous peroxidase activity, an antigen retrieval step was performed with either high-temperature citrate buffer (pH = ...trypsin. The following primary antibodies were used in this study: a ... See full document
9
Characterization, subsite mapping and N-terminal sequence of miliin, a serine-protease isolated from the latex of Euphorbia milii
... for the normal functioning of multicellular ...of the plant life cycle involving a variety of functions, from mobilization of storage pro- teins during seed germination to the initiation of ... See full document
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