[PDF] Top 20 Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp

... Reference genes are commonly used for normalization of target gene expression during RT-qPCR ...housekeeping genes or reference genes have been identified to ... See full document

... 11 genes used for the analysis showed high expression stability and presented an M value lower than the cutoff established by Vandesompele et ...stable reference genes were not ... See full document

... gene expression analysis requires one or several reference gene(s) acting as normalization ...factor(s). In order to facilitate gene expression studies in sugarcane (Saccharum ... See full document

... research in gene expression, and it is vital to choose appropriate housekeeping genes (HKGs) as reference genes to obtain correct ...purpose of this study is to determine stably ... See full document

... applicability of this candidate reference gene set on different experimental conditions, we performed a similar evaluation of suitable reference genes in acute phase of ... See full document

... quantification of a target gene transcript, requiring the use of appropriated reference genes for data ...relative expression of target tomato (Solanum lycopersicum L.) ... See full document

... and analysis of gene function, an important part of any fol- low-up study, requires the appropriate selection of reference ...for RT-qPCR is critical for accurate ... See full document

... Analysis of gene expression involved in cancer tumorigenesis and metastasis is key to tailoring individual treatments and RT-qPCR can assist with ...this. In ... See full document

... expressions of the genes that showed the highest differential methylation status were investigated by ...included in this transcription experiment. Due to the limited amount of tissue ... See full document

... specificity of the primer pairs used in this study, melting curve analysis was performed after 40 cycles of ...specificity of the amplicon. No signal was detected in the negative ... See full document

... use of inappropriate references can dramatically change the interpretation of the expression pattern of a given target gene ...usefulness of the validated candidate reference ... See full document

... selection of adequate reference genes are prerequisites for gene expression ...used in a laboratory routine for gene expression analysis. In this study, an ... See full document

... either of the two softwares ...candidate genes have different expression stabilities under different conditions of stresses, and using the two different softwares may lead to different ... See full document

... of reference genes required for normalization is two ...use of three genes for the first two conditions and five for latter is necessary ...ranking of the three algorithms as ... See full document

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... preparation of a miRNA for pharmacological applications, certain characteristics have to be considered like the quantity required, the product quality, stability, safety and biological ...production of ... See full document

... frozen in liquid nitrogen and ground into a fine powder using mortar and ...3.5ml of extraction buffer containing ...volume of phenol pH ...volume of SEVAG - phenol: ... See full document

... used in creation of a knowledge base for CAPCAST system, based on different types of input data under the conditions specified by the problem do- main, that is, selection of materials for the ... See full document

... regions of Europe, Asia and North America, and the mountains of Central ...analyses of its alkaloid (Philipov et ...tolerance of four varieties of Iceland poppy to low temperatures ... See full document

... walls of buildings. It could be decorative niches (of spiral form in the Hall of Warriors), small rectangular "shelves" (in the Hall of Victories), rectangular niches ... See full document