[PDF] Top 20 Accurate detection and quantification of the fish viral hemorrhagic Septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay.
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Accurate detection and quantification of the fish viral hemorrhagic Septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay.
... issues, the present research describes and evaluates a new, accurate, fast, and highly reliable assay to diagnose and quantify ...This assay uses Standardized ... See full document
12
Development and optimisation of a group-specific real-time RT-PCR assay for the broad detection of the Simbu serogroup orthobunyaviruses
... distribution and have been divided phylogenetically into two different clades, designated A, those associated with clinical disease in humans, and B, associated with abortion and ... See full document
112
Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses.
... spite of modern intensive care, especially some HFVs can be transmitted from human to human and consequently threat to cause epidemics with high mortality rates ...to the unspecific clinical ... See full document
11
Detection of infectious laryngotracheitis virus by real-time PCR in naturally and experimentally infected chickens.
... disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens ... See full document
10
Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
... on the complete genome sequence of ...First, the sequences were subjected to BLAST ® ...fication of genomic differences (Altschul et al., 1997). Then, the selected target sequences ... See full document
7
Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis
... glycosylase, and stabilizers) (Invitrogen, CA), six pairs of primers at their respective concentra- tions (Table 3), and 2 l of the template by using the DNA Engine Opticon 2 ... See full document
4
Detection of A. phagocytophilum and E. chaffeensis in patient and mouse blood and ticks by a duplex real-time PCR assay.
... by the Dr Didier Raoult at the WHO Collaborative Center for Rickettsial Reference and Research, Marseille, France); Anaplasma phagocytophilum strains Webster, MRK, Slovenia and MD, which were ... See full document
7
Rev. Soc. Bras. Med. Trop. vol.50 número4
... chikungunya virus (CHIKV), an Aedes ...sp.-transmitted virus. This disease has been diagnosed in thousands of cases in the Americas, particularly in Brazil, in recent years, and there ... See full document
5
Quantification of trace-level DNA by real-time whole genome amplification.
... length of the random sequence in the middle of the DOP primer is also an important determinant of DOP-PCR efficiency because it affects the frequency and ... See full document
8
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada
... 1-step real-time PCR protocol was used following manufacturer’s directions (OpenArray, Life Technologies ...selected PCR assays (pri- mers and probe) were inserted in duplicate in ... See full document
8
The comparison of the structure and microhardness of the tool steel C90 and HS 6-5-2 remelted with the electric arc
... Machine and tools elements made of the steel C90 and HS 6-5-2 immediately after the conventional hardening, need the tempering ...During the tempering, there is a ... See full document
4
Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
... Regarding the temperature treatment of broiler carcass, it is interesting to observe that despite the higher frequency of carcasses contaminated by Campylobacter ...(P>0.05). The ... See full document
7
Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares...
... Nilsson et al. em 1999, do Grupo Escandinavo de Sarcomas, estudaram o padrão de fusão SS18-SSX, comparando com a evolução e a porcentagem de positividade para o Ki-67 (102). Os autores concluíram ser significantemente ... See full document
102
Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients
... Influenza detection using either method was 7.6% in the hematopoietic stem cell transplant group and ...in the kidney transplant patient group. Influenza detection by ... See full document
4
Histamine release and fluorometric assay
... increased the histamine release in guinea pig lung suspension containing mast ...since the histamine release induced by propolis was not inhibited by sodium cyanide (data not ...only with a high ... See full document
8
Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR
... Duplex real-time RT-PCR reactions either from different plants or from an individual, double infected plant, successfully detected the viruses mentioned before (GLRaV-1 not included), ... See full document
8
A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... places of Brazil have shown neutralising antibodies to PIRYV in more than 10% of the ...in the laboratory, we know that PIRYV produces an acute febrile illness (Pinheiro et ...sera of ... See full document
6
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System.
... capable of causing a variety of acute illnesses, including the most commonly reported acute gastrointesti- nal ...via the fecal-oral route either by person-to-person con- tact or by ingestion ... See full document
17
Real-Time PCR for Measles Virus Detection on Clinical Specimens with Negative IgM Result in Morocco.
... In the current study, 65% (CI 63–67%) of samples are collected within four days after rash onset, which may explain the higher rate of Measles IgM negative samples and the ... See full document
10
A search for RNA insertions and NS3 gene duplication in the genome of cytopathic isolates of bovine viral diarrhea virus
... infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal ...Both the original virus (ncpBVDV) and an ... See full document
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