[PDF] Top 20 Multiplex real-time PCR diagnostic of relapsing fevers in Africa.
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Multiplex real-time PCR diagnostic of relapsing fevers in Africa.
... developed real-time PCR was validated against large number of samples from areas endemic for diverse borreliae causing human infection in Africa, showing 100% sensitivity and ... See full document
5
Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis
... episodes of circulating bacterial and/ or fungal DNA detected using SeptiFast than episodes in which microorganisms were detected using BC, as observed in other studies ...[28,29]. In ... See full document
8
A Multiplex Real-Time PCR Assay to Diagnose and Separate Helicoverpa armigera and H. zea (Lepidoptera: Noctuidae) in the New World.
... used in this study are summarized in Table 1. A total of 452 Heliothinae represent- ing 18 species were used to develop the real-time PCR ...interceptions in the ... See full document
18
Detection and typing of highly pathogenic porcine reproductive and respiratory syndrome virus by multiplex real-time rt-PCR.
... losses in the pig industry worldwide, and PRRS viruses (PRRSV) are classified into the two distinct genotypes ‘‘North American (NA, type 2)’’ and ‘‘European (EU, type ...1)’’. In 2006, a highly pathogenic ... See full document
9
A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks
... success of MG and MS control programs de- pends on accurate and fast diagnostic ...that real time PCR assays (including multiplex procedures) are effective tools for the ... See full document
6
Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
... levels of mtDNA were detected in plasma of prostate cancer patients using quantitative real-time PCR amplification (Mehra et ...mtDNA in serum of patients with ... See full document
5
Optimized Pan-species and speciation duplex real-time PCR assays for Plasmodium parasites detection in malaria vectors.
... context of malaria elimination (eradication) policy, it is essential to develop reliable diagnostic techniques for detecting Plasmodium spp infections in humans and in the ...aim of ... See full document
8
Implementation and Evaluation of a Fully Automated Multiplex Real-Time PCR Assay on the BD Max Platform to Detect and Differentiate Herpesviridae from Cerebrospinal Fluids.
... Routine diagnostic assay for the detection of HSV1, HSV2, VZV The routine diagnostic assay is carried out in the DAkkS (Deutsche Akkreditierungsstelle GmbH) accredited laboratory of the ... See full document
12
Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses.
... plex PCR panel that detects the amplification of various viral gene fragments ...a multiplex PCR reaction fol- lowed by denaturation of the double stranded molecules into single ... See full document
9
DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR ASSAYS FOR THE DETECTION OF NOSOCOMIAL PATHOGENS Deepa Anbazhagan
... source of morbidity and ...assays of nosocomial pathogens for better prognosis and initiation of antimicrobial therapy in ...patients. In this study, we present the development ... See full document
11
Application of time-series analysis for prediction of molding sand properties in production cycle
... noise. In the second case the modeling would be certainly ...One of them is the non-parametric runs-test that verifies a randomness hypothesis for a binary-valued data sequence; in case of ... See full document
5
UNIVERSIDADE DE BRASÍLIA FACULDADE DE AGRONOMIA E MEDICINA VETERINÁRIA PROGRAMA DE PÓS-GRADUAÇÃO EM SAÚDE ANIMAL REAÇÃO EM CADEIA DA POLIMERASE (PCR) DE SANGUE
... da PCR do sangue periférico e esplênico apresentaram baixa concordância o que corrobora com a necessidade de se utilizar mais de uma técnica para diagnosticar animais ... See full document
67
A real-time PCR antibiogram for drug-resistant sepsis.
... molecular diagnostic techniques for susceptibility testing of septicemia rely on genotyping for the presence of known resistance ...resulted in the widespread and deleterious use of ... See full document
6
Different methods of real-time PCR for detection of pseudorabies virus
... disease of great animal health and economic importance in swine ...aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the ... See full document
7
Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
... effective in removing heat and toxic substances. Most of traditional Chinese medicines are been distributed in desert regions, which leads to their ...shortage. In recent years, there has been ... See full document
9
Comparative Study of a Real-Time PCR Assay Targeting senX3-regX3 versus Other Molecular Strategies Commonly Used in the Diagnosis of Tuberculosis.
... number of patients included in the study was not very large, it was nonetheless no smaller than many of the studies included in a recent meta-analysis about GX published by the Cochrane ... See full document
14
Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
... treatment of broiler carcass, it is interesting to observe that despite the higher frequency of carcasses contaminated by Campylobacter ...that real-time PCR detects the genetic ... See full document
7
Multispacer sequence typing relapsing fever Borreliae in Africa.
... ,0.2% of the total genome ...representative of the whole ...representative of the whole genome. Based on this spacer sequencing, a total of 61 RF strains could be separated into 12 ... See full document
6
Real-time PCR in infectious uveitis as an alternative diagnosis
... shown in table 3, ...detected in the vitreous and aqueous in HIV ...gondii in the vitreous humor. The CMV, HSV2 and VZV were found only once in the aqueous ...once in the ... See full document
4
Novel set of real-time PCR primers for simultaneous detection of Liberibacter
... sensitivity of the qPCR assay development in this study was tested against a standard qPCR method- ology and the Ct values of the p3G primers were com- pared with PCR-primers targeting the 16S ... See full document
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