[PDF] Top 20 Recombinant proteins in differentiation of stem cells
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Recombinant proteins in differentiation of stem cells
... resuspended in 10 mL of ice cold STE Buffer (10 mM Tris-HCl, pH ...mM of DTT, 1 mM of PMSF and 1.5 mL of 10% Sarkosyl (final concentration 1,5%) were ...time of 1 min (On 7’’, ... See full document
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Histone demethylase Jumonji D3 (JMJD3) as a tumor suppressor by regulating p53 protein nuclear stabilization.
... accumulation of repressive histone modifications, such as tri-methylated histone 3 lysine 27 (H3K27me3) mediated by histone methyltransferases such as EZH2, resulting in a histone methylation mediated block ... See full document
14
Role of TRPV1 in the Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes.
... member of the TRP super family of ion ...topology of six transmembrane segments (S1–S6) and a pore region between S5 and S6 ...expressed in variety of different tissues and cell types ... See full document
12
Establishment and evaluation of flexible insect cell lines for rapid production of recombinant proteins
... therapeutic proteins (Kito, Itami et al. 2002; Huang, Li et al. 2007). In particular, recombinase- mediated cassette exchange (RMCE) systems make use of site-specific recombinases, such as flipase ... See full document
110
Production of recombinant proteins in Escherichia coli
... sequence in the ribo- some-binding sites (RBS) of bacteriophage mRNAs and suggested that this region interacts with the complementary 3’ end of the 16S rRNA during translation initiation (Shine and ... See full document
12
Motoneuron differentiation of induced pluripotent stem cells from SOD1G93A mice.
... properties of both ALS-iPS and C57-iPS derived motoneurons in ...RNAs of motoneurons derived from ALS-iPS and C57- iPS cells were extracted using TRIzol (Invitrogen) ... See full document
8
Caracterização, criopreservação e diferenciação in vitro de células tronco, derivadas de amniótica e gelatina de Wharton canina, visando a formação de um banco de células tronco
... Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell ...Journal of Translational Medicine, ...Effect of anatomical origin and ... See full document
101
Rev. bras. ortop. vol.46 número6 en a04v46n6
... tissue of murinae and humans, after neuronal induction, presented morpho- logy typical of nerve cells, which were positive for immunocytochemical expression of GFAP, nestin and neuron-specific ... See full document
7
Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
... co-cultured cells were harvested out for the future ...the cells were transduced by virus before co-culturing on HAE or ...293T cells were transfected by a mixture consisting of pLox-EWGFP, ... See full document
9
Mesenchymal stem cell-based differentiation of smooth muscle cells
... lack of expression on CV-MSC and UC-MSC [51, 53]; usually this marker is report to be use to exclude the BM-MSC from the other MSC sources [103, ...level in the three MSC donors of different ... See full document
119
HOX gene analysis in the osteogenic differentiation of human mesenchymal stem cells
... patterns of the HOX genes during osteogenic differentiation may reveal the signal pathway of osteogenesis and may also help in the potential therapeutic application of ...profile ... See full document
9
Molecular mechanisms triggering differentiation of the embryonic endodermal stem cells
... described in section ...temperature in a Table-shaker see-saw rocker SSL4 (Stuart Scientific, GB) at 40 ...presence of villus and/or crypts. Solutions rich in villus debries were discarded and ... See full document
124
Culture conditions affect cardiac differentiation potential of human pluripotent stem cells.
... positive cells could be detected in long-term culture of hPSCs on Matrigel matrix together with mTeSR1 medium than in MEF and SNL feeder cell ...expression of PAX-6, Musashi and NF-68 ... See full document
13
Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits
... signs of peritonitis were observed caused by dehiscence of urinary bladder suture or rejection of the implant in any ...used in the CGcy, is preferred by some researchers as an ... See full document
7
Stem cells and bioengineering in the current context of dentistry and general health / Células-tronco e bioengenharia no contexto atual da odontologia e saúde geral
... rich in nutritional substances and favors the transport of nutrients and metabolic waste to ...cells. In DSC, the matrix components activate the morphogens, which are secreted extracellularly ... See full document
18
Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
... hematopoietic stem cell mobilization. 12,14 SCF is also useful in gene therapy as hematopoietic cells exposed to SCF either in vivo or in vitro are more efficiently transduced by ... See full document
5
Integrin-associated protein promotes neuronal differentiation of neural stem/progenitor cells.
... use of experimental animals in this study was conducted in accordance with the recom- mendations in the Guiding Principles for the Care and Use of The Japanese Pharmacological ...number ... See full document
10
Braz. J. Pharm. Sci. vol.51 número3
... member of the most biologically active forms of ...growth in a wide spectrum of cancer cell lines. The mechanisms of anti- cancer efects underlying RA appear to be associated with its ... See full document
10
Rev. Bras. Hematol. Hemoter. vol.30 suppl.2
... accumulation of malignant plasma cells within the bone marrow (BM). MM cells interact with the microenvironment and induce pathological modifications that in turn support the growth and ... See full document
3
Braz. Dent. J. vol.28 número6
... properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances ... See full document
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