[PDF] Top 20 A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... places of Brazil have shown neutralising antibodies to PIRYV in more than 10% of the ...sera of patients with acute febrile illness by the real-time RT- PCR for VSV, but all samples ... See full document
6
An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrepCobas TaqMan HBV tests for the quantification of hepatitis B virus DNA
... F, and only one from genotype ...detected and quantified by our ...performance of our in-house test was independent of genotype and ... See full document
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Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
... morbidity and mortality of upper and lower acute respiratory infec- tions (ARIs) for all age ...aim of this study was to determine the frequencies for a large range of respira- tory ... See full document
6
Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, Southern Brazil
... presence of HEV in swine feces, manure and effluents from slaughterhouses (Kasorndorkbua et ...63% of Brazilian swine herds (Gardinalli et ...9.6% of bile samples as well as in 50% of ... See full document
6
Rev. Soc. Bras. Med. Trop. vol.47 número3
... genotype and quantify hepatitis C virus (HCV) RNA in clinical samples have been extensively ...cation and genotyping) are also available, all of which are typically based on polymerase ... See full document
8
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
... transcription-quantitative polymerase chain reaction (RT-qPCR) RNA was extracted using the UNIQ-10 column TRIzol kit (Shanghai Sangon Biotech, Shanghai, China) and treated with ...total ... See full document
9
Development of a quantitative competitive reverse transcriptase polymerase chain reaction for the quantification of growth hormone gene expression in pigs
... Nielsen and Larsen (1991) studied RFLPs (restriction fragment length polymorphisms) of the GH gene in Danish and Swedish pig populations, and found pGH polymorphisms with DraI and TaqI ... See full document
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DEVELOPMENT OF TEST KIT FOR DETECTION OF PANDEMIC STRAIN INFLUENZA VIRUS A (H1N1 2009) BY REAL TIME POLYMERASE CHAIN REACTION
... experience of fighting the flu, accumula- ted over the years, has shown that to develop and deliver effective preventive measures necessary to build a system of permanent monitoring for influenza ... See full document
7
Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients
... strategies and antiviral treatment, but the benefits of this progress are related to improved diagnostic ...diagnosis of influenza virus that differ with respect to sensitivity, cost, and ... See full document
4
In fluence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction
... (made of medical absorbent cotton, urethral swabs from males and vaginal swabs from females) were collected from all participants and placed into separate transport tubes (Yangzhou JiKang Medical ... See full document
6
Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
... advantage of the in silico approach was the considerable reduction in the time spent to validate the in vitro ...concentrations, and the annealing and elongation temperatures required ... See full document
9
Analysis of Respiratory Syncytial Virus in Clinical Samples by Reverse Transcriptase-Polymerase Chain Reaction Restriction Mapping
... aim of this study was to develop a polymerase chain reaction (PCR) for the detection of respi- ratory syncytial virus (RSV) ...sequences and se- lected from conserved ... See full document
6
Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction
... characterization of DEN-1 and DEN-2 viruses by the agarose gel analysis of the amplified ...(41/100) of cases studied. Eleven DEN-1 and 30 DEN-2 strains were demonstrated in acute ... See full document
5
Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
... CONTEXT AND OBJECTIVE: Recent studies have indicated that vitiligo areas contain inactive or dormant ...presence and indicative of active metabolic state. The aim of this study was to compare ... See full document
5
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys
... development of safe live attenuated flavivirus vaccines one of the main properties to be established is viral ...used real-time reverse transcriptase-polymerase ... See full document
11
Valéria CS Pinheiro+ , Wanderli P Tadei, Patrícia MSS Barros, Pedro FC Vasconcelos, Ana Cecília R Cruz
... DENV1 and DENV2 serotypes, that were responsible for the previous dengue epidemic episodes in Manaus, were displaced by DENV3, the last serotype introduced in ...part of the population had already been ... See full document
7
Braz. j. . vol.76 número5 en v76n5a08
... introduction of polychemotherapy (PCT/ WHO) in the 1980s, new diagnostic tools for an early diagnosis of leprosy have been ...in and out of households, together with BCG vac- cination ... See full document
9
New Method for Early Detection of two Random Amplified Polymorphic DNA (RAPD) Groups of Staphylococcus aureus Causing Bovine Mastitis Infection in Paraná State, Brazil
... specific and sensitive detection of mastitis pathogens directly in ...Germany and Denmark, but no cross reactivity could be observed investigating staphylococcal reference ...direct and ... See full document
8
Molecular and serological diagnosis of hepatitis e virus in swine and chicken
... rabbits and wild boar should be considered new genotypes or subtypes (Zhao et ...sequencing of different genomic regions and different fragment sizes in addition to the lack of commonly agreed ... See full document
53
Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples
... limitations of viral culture and IFA prompted us to perform this study in which we evalu- ated a commercially available ICA-BD to determine if it can be used as an alternative diagnostic method for ... See full document
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