[PDF] Top 20 Verification of IgY specificity by ELISA
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Verification of IgY specificity by ELISA
... source of antibody production. IgY binds neither to Fc receptors of mammals nor to the mammalian rheumatoid factor, as well as not activating the complement factor, features that reduce ... See full document
9
Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
... sensitivity, specificity and high cross-reactivity presented by the crude Toxocara antigen, we performed the identification, separation and testing of the semi-purified ...sis of the crude ... See full document
7
Use of ELISA based on NcSRS2 of Neospora caninum expressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
... caused by the protozoon Neospora caninum that leads to significant economic losses in many ...use of the recombinant protein NcSRS2 of ...the ELISA test yielded specificity of ... See full document
7
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
... possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of ... See full document
4
Elisa de captura com IgY para quantificação de acetato de lupeol em Vernonia scorpioides lam. Pers (asteraceae).
... (capture IgY-ELISA) was carried out to detect Lupeol acetate (LAc) from Vernonia scorpioides ...recovered by cleanup columns containing LAc-BSA coupled to ...capture IgY- ELISA efficacy ... See full document
6
Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
... time, IgY antibodies against ...yolk of chickens injected with D. acutus venom multiple times. By three steps, including caprylic acid extraction, ammonium sulfate precipitation and affinity ... See full document
9
Avaliação de um ELISA para detecção de anticorpos contra Babesia bigemina em bovinos e sua aplicação em um inquérito sorológico no Brasil.
... comparison of ELISA and indirect fluorescent antibody test (IFAT) in detecting antibodies against ...isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most ... See full document
5
Comparison of dot-ELISA and standard ELISA for detection of
... latedfromaBrazilianepidemic).Thedot-ELISAisbasedonthesameprinciplesasthestandard ELISA and is useful for detection of ...serum of patients with meningococcal ... See full document
6
Evaluation by ELISA of Anisakis simplex Larval Antigen Purified by Affinity Chromatography
... bands of 40 and 14 kDa. In the A. simplex PAS antigen, the proteins were of 120, 66- 45, 40, 31-21 and 14 kDa with elevated concentrations of these specific proteins that those observed in the non- ... See full document
6
Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA) to Detect the Cytokinins in Gerbera jamesonii Plantlets
... tissue of plantlet fragments which was diluted at 1:10 in methanol, macerated for 60 minutes at 4 °C, filter-sterilized ...% of Tween 20 (PBST). Each sample assayed was added to equal volume of 1:400 ... See full document
6
Multi-country evaluation of the sensitivity and specificity of two commercially-available NS1 ELISA assays for dengue diagnosis.
... sensitivities of the reference RT/PCR methods employed for dengue ...magnitudes of virus burden in patients with different ...days of illness in patients at risk of developing severe ... See full document
10
CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
... the specificity of the IgY antivenom to different coral snake venoms as well as other Venezuelan venomous ...the IgY coral snake venom antivenom was able to recognize all four coral snake ... See full document
6
Laboratory investigations on the diagnosis of tuberculosis in the malnourished tribal population of melghat, India.
... and specificity of a PCR system for the detection of ...(20/41) of the active TB ...positivity of PCR assay in TB group was more significant than control ...TB-Ag ELISA technique ... See full document
8
Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
... specific ELISA reactions for the diagnosis of PCM, we tried to use purified gp43 antigen, the immunodominant molecule which is specific when used in im- munodiffusion tests, treated with sodium ... See full document
3
DNA vaccine-generated duck polyclonal antibodies as a postexposure prophylactic to prevent hantavirus pulmonary syndrome (HPS).
... a-ANDV IgY/IgYDFc, could protect against lethal HPS to levels similar to human ...dose of 12,000 NAU/kg IgY/ IgYDFc delivered five days postexposure conferred significant protection in both the ... See full document
11
Evaluation of a Commercial ELISA Kit (IDEXX) to Differentiate AI Virus-infected Poultry from AI- Vaccinated Poultry (DIVA)
... control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected ...use of AI vaccination in poultry would have greater ... See full document
6
Crude Antigen from Taenia crassiceps Cysticercus Used as Heterologous Antigen in ELISA and in EITB for Neurocysticercosis Diagnosis of Patients from Paraná-Brazil
... reactivity of serum or CSF from patients with inactive, ...explained by the presence of antibodies that could persist for a long time after the parasite was eliminated by immune mechanisms ... See full document
12
Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis
... serodiagnosis of human toxocariasis, a sensitive and specific enzyme-linked immunoelectrotrans- fer blot (EITB-IgG) test was developed and evaluated using Toxocara canis larvae excretory-secretory antigens for ... See full document
8
Lipopolysaccharide Specific Immunochromatography Based Lateral Flow Assay for Serogroup Specific Diagnosis of Leptospirosis in India.
... pain of the abdomen, breathlessness, subconjunctival haemor- rhages, and jaundice with acute renal failure) leptospirosis cases were ...phase of illness (0 to 10 days after onset of the disease). ... See full document
13
Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil
... aim of this study was to evaluate the presence of anti-Toxocara antibodies in naturally infected broiler chickens (n = 189) from the state of Paraná, southern ...system by small family farmers ... See full document
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